查询词典 somatic effects

The effects and mechanism of GABAergic neurons, NOergic neurons, opioid peptide and cyclic adenosine monophosphate in the nucleus reticularis thalami on sleep-wakefulness cycle of rats and the effects and mechanism of the 5-HTergic nerve fibers project from the nucleus raphes dorsalis to RT on sleep-wakefulness cycle of rats were investigated with the methods of brain stereotaxic, nucleus spile, microinjection and polysomngraphy.1. The effects of GABAergic neurons in RT on sleep-wakefulness cycle of rats1.1 Microinjection of 3-mercaptopropionic acid (3-MP, a kind of glutamate decarboxylase inhibitor) into RT. On the day of microinjection, sleep only decreased a litter. On the second day, sleep marked decreased and wakefulness marked increased. On the third and fourth day, sleep and wakefulness stages resumed to normal.1.2 Microinjection of gamma-amino butyric acid (GABA 1.0μg) into RT enhanced sleep and reduced wakefulness compared with control; while microinjection of L-glutamate (L-Glu, 0.2μg) decreased sleep and increased wakefulness; microinjection of bicuculline (BIC, 1.0μg), a GABAA receptor antagonist, enhanced wakefulness and reduced sleep; microinjection of baclofen (BAC, 1.0μg), GABAB receptor agonist, had the same effects as GABA.2. The effects of NOergic neurons in RT on sleep-wakefulness cycle of rats2.1 Microinjection of L-arginine (L-Arg, 0.5μg) into RT decreased sleep compared with control, but there were on statistaical difference between L-Arg group and control; while microinjection of sodium nitroprusside (SNP, 0.2μg), a NO donor into RT, sleep marked decreased and wakefulness marked increased. Microinjection of nitric oxide synthase inhibitor, N-nitro-L-arginine (L-NNA, 2.0μg) into RT enhanced sleep and reduced wakefulness.2.2 After simultaneous microinjection of L-NNA (2.0μg) and SNP (0.2μg) into RT, SNP abolished the sleep-promoting effect of L-NNA compared with L-NNA group; after simultaneous microinjection of L-NNA (2.0μg) and L-Arg(0.5μg) into RT, we found that L-NNA could not blocked the wakefulness-promoting effect of L-Arg.3. The effects of opioid peptide in RT on sleep-wakefulness cycle of rats3.1 Microinjection of morphine sulfate (MOR, 1.0μg) into RT increased wakefulness and decreased sleep compared with control; while microinjection of naloxone hydrochloride (NAL, 1.0μg), the antagonist of opiate receptors, into RT, enhanced sleep and reduced wakefulness.3.2 After simultaneous microinjection of MOR (1.0μg) and NAL (1.0μg) into RT, the wakefulness-promoting effect of MOR and the sleep-promoting effect of NAL were not observed compared with control.4. The effects of cAMP in RT on sleep-wakefulness cycle of rats Microinjection of cAMP (1.0μg) into RT increased sleep and decreased wakefulness compared with control; microinjection of methylene blue (MB,1.0μg) into RT enhanced sleep and reduced wakefulness compared with control.5. The effects of the 5-HTergic nerve fibers project from DRN to RT on sleep-wakefulness cycle of rats5.1 When L-Glu (0.2μg) was microinjected into DRN and normal sodium (NS,1.0μg) was microinjected into bilateral RT. We found that sleep was decreased and wakefulness was increased compared with control; when L-Glu (0.2μg) was microinjected into DRN and methysergide (MS,1.0μg), a non-selective 5-HT antagonist, was microinjected into bilateral RT, We found that sleep was enhanced and wakefulness was reduced compared with L-Glu group.5.2 When p-chlorophenylalanine (PCPA, 10μg) was microinjected into DRN and NS (1.0μg) was microinjected into bilateral RT, We found that sleep was increased and wakefulness was decreased compared with control; microinjection of 5-hydroxytryptaphan (5-HTP, 1.0μg), which can convert to 5-HT by the enzyme tryptophane hydroxylase and enhance 5-HT into bilateral RT, could block the effect of microinjection of PCPA into DRN on sleep-wakefulness cycle.

本研究采用脑立体定位、核团插管、微量注射、多导睡眠描记等方法，研究丘脑网状核(nucleus reticularis thalami，RT)中γ-氨基丁酸(gamma-amino butyric acid ，GABA)能神经元、一氧化氮(nitrogen monoxidum，NO)能神经元、阿片肽类神经递质、环一磷酸腺苷(cyclic adenosine monophosphate，cAMP)及中缝背核(nucleus raphes dorsalis，DRN)至RT的5-羟色胺(5-hydroxytryptamine，5-HT)能神经纤维投射对大鼠睡眠-觉醒周期的影响及其作用机制。1 RT内GABA能神经元对大鼠睡眠-觉醒周期的影响1.1大鼠RT内微量注射GABA合成关键酶抑制剂3-巯基丙酸(3-MP，5μg)，注射当天睡眠时间略有减少，第二日睡眠时间显著减少，觉醒时间明显增多，第三、四日睡眠和觉醒时间逐渐恢复至正常。1.2大鼠RT内微量注射GABA受体激动剂GABA( 1.0μg)后，与生理盐水组比较，睡眠时间增加，觉醒时间减少；而RT内微量注射L-谷氨酸(glutamic acid， L-Glu， 0.2μg)后，睡眠时间减少，觉醒时间增加；RT内微量注射GABAA受体阻断剂荷包牡丹碱(bicuculline，BIC，1.0μg)后，睡眠时间减少，觉醒时间增加；RT内微量注射GABAB受体激动剂氯苯氨丁酸(baclofen，BAC，1.0μg)后，产生了与GABA相似的促睡眠效果。2 RT内NO能神经元对大鼠睡眠-觉醒周期的影响2.1大鼠RT内微量注射NO的前体L-精氨酸(L-Arg，0.5μg)后，与生理盐水组对比，睡眠时间略有减少，但无显著性意义；而RT内微量注射NO的供体硝普钠(Sodium Nitroprusside，SNP，0.2μg)后可明显增加觉醒时间，缩短睡眠时间；微量注射一氧化氮合酶抑制剂L-硝基精氨酸(L-arginine，L-NNA，2.0μg)后，引起睡眠时间增多，觉醒时间减少。2.2大鼠RT内同时微量注射L-NNA(2.0μg)和SNP(0.2μg)后与L-NNA组比较发现SNP逆转了L-NNA的促睡眠作用；RT内同时微量注射L-NNA(2.0μg)和L-Arg(0.5μg)后，与L-NNA(2.0μg)组比较发现L-Arg可以增加觉醒而缩短睡眠，其促觉醒作用未能被NOS的抑制剂L-NNA所逆转。3 RT内阿片肽对大鼠睡眠-觉醒周期的影响3.1大鼠RT内微量注射硫酸吗啡(morphine sulfate，MOR，1.0μg)后与生理盐水组对比，睡眠时间减少而觉醒时间增加； RT内微量注射阿片肽受体拮抗剂盐酸纳洛酮(naloxone hydrochloride，NAL，1.0μg)后与生理盐水组比较，睡眠时间增加而觉醒时间减少。3.2大鼠RT内同时微量注射MOR(1.0μg)和NAL(1.0μg)后，与生理盐水组对比，原有的MOR促觉醒效果和NAL的促睡眠效果都没有表现。4 RT内环一磷酸腺苷信使对大鼠睡眠-觉醒周期的影响大鼠RT内微量注射cAMP(1.0μg)后与NS(1.0μg)组比较，睡眠时间增多而觉醒时间减少；RT内微量注射亚甲蓝(methylene blue，MB，1.0μg)后，与NS组比较，睡眠时间增多而觉醒时间减少。5中缝背核投射到丘脑网状核的5-羟色胺能神经纤维对大鼠睡眠-觉醒周期的影响5.1大鼠DRN内微量注射L-Glu(0.2μg)，同时在双侧RT内微量注射NS (1.0μg)后，与对照组(DRN和双侧RT注射NS， 0.2μg)比较，睡眠时间减少，觉醒时间增多；大鼠DRN内微量注射L-Glu(0.2μg)，同时在双侧RT内微量注射二甲基麦角新碱(methysergide， MS， 1.0μg )后，与对照组(DRN注射L-Glu 0.2μg，双侧RT注射NS 1.0μg)比较，睡眠时间增多，觉醒时间减少。5.2大鼠DRN内微量注射对氯苯丙氨酸(p-chlorophenylalanine，PCPA，10μg)，同时在双侧RT内微量注射NS (1.0μg)后，与对照组(DRN和双侧RT注射NS， 1.0μg)比较，睡眠时间增多，觉醒时间减少；大鼠DRN内微量注射PCPA(10μg)，产生睡眠增多效应后，在双侧RT内微量注射5-羟色胺酸(5-hydroxytryptaphan ， 5-HTP， 1.0μg )后，与对照组(DRN注射PCPA 10μg，双侧RT注射NS 1.0μg)比较，睡眠时间减少，觉醒时间增多。

In this study, the peanut (Arachis hypogaea L.) cultivars were used as materials to investigate the effect of 2,4-dichlorophenoxyacetic acid(2,4-D) on somatic embryogenesis and of naphthaleneacetic acid and ascorbic acid on rooting, then to find a efficient protocols for regeneration of plantlet via somatic embryogenesis.

本试验以落花生栽培种之未熟胚为材料，探讨2，4-dichlorophenoxyacetic acid(2，4-D)诱导体胚，及naphthaleneacetic acid与抗坏血酸促进发根的效果，期望建立经由体胚形成再生植株之方法。

This article reviews recent progresses in research on plant somatic embryogenesis based on our experiments and research reports by others.⑴ Changes in ultrastructure: Once a plant somatic cell turns into embryonic state, the number of organelles increases significantly, with mitochondrial crista much developed and some of them in the dividing state.

本文以我们的研究结果为基础，并结合国内外近几年有关研究报道，对植物体细胞胚发生中的超微结构和ATP酶活性时空分布动态及内源激素的变化和作用进行专题评述。

Seed explants of Eleutherococcus senticosus produced somatic embryos directly ongrowth regulator-free medium. The results were showed that use low temperature stressmethod to relieve the Eleutherococcus senticosus seed conformation dormancy andphysiological dormancy, to induce seed germinate. Proliferation of Eleutherococcus senticosussomatic embryo without any plant growth regulathors was better in liquid medium containing1/3MS+sucrose 3%, and better in solid medium containing 1/3MS+sucrose 1%.Eleutherococcus senticosus secondary embryo was stick to matrix through espial of cytology.Eleutherococcus senticosus somatic embryo was cultivated some times to form immature frondin liquid medium, then transfer into solid medium to form full plant. The experiment wasindicated that much more Eleutherococcus senticosus immature frond can be cultured inbioreactor, so large-scale production in bioreactor culture can be done.

研究结果表明：低温胁迫处理刺五加种子可以打破种子的形态休眠和生理休眠，促使种子发芽：在不添加任何植物激素的培养基中诱导刺五加体胚形成，其中，1／3MS+3％蔗糖的培养基搭配有利于液体培养的刺五加体胚增值，1／3MS+1.0％蔗糖的培养基搭配有利于固体培养的刺五加体胚增值；在进行体细胞胚增殖时，体细胞胚继代三周后表面开始形成很多次生胚，对刺五加次生胚进行细胞学观察，发现刺五加次生胚与母体只是粘贴在一起；刺五加体胚需在液体培养基中培养一段时间形成幼植物体，实验表明可以通过生物反应器培养大量的刺五加幼植物体，然后转入固体培养基中形成完整植株，因此可以通过生物反应器培养进行规模化生产。

Ginseng can be regenerated through organogenesis or somatic embryogenesis and indirect somatic embryogenesis is mainly used for its regeneration.

遗传改良是人参育种的重要手段之一，而遗传转化和再生体系的建立是开展人参遗传改良工作的前提和基础。

Axial elongation of inner isodiametric cells of the globular somatic embryo followed by the change in the growth axis of the procambium was an important event in heart-shaped or oblong-stage somatic embryo.

球形胚内部等径细胞轴向延长，接着前形成层生长轴发生变化，这些是心形胚或椭圆形体胚中的重要事件。

It involves a somatic reflex arc with a modified efferent branch which passes the somatic motor impulses to the bladder and initiates autonomic bladder detrusor contraction.

它包含一个体反射弧，并将体反射运动冲动经异化的运动传出支传入膀胱，引起膀胱的自主性收缩。

The somatic motor axons can be regenerated into the parasympathetic endoneurial tubes of autonomic nerve.Using the survived somatic reflex under the horizon of spinal cord injury to reconstruct the bladder autonomic reflex arc by intradural microanastomosis of ventral root is practical in the canine model and may have a potential of clinical application.

体神经的运动传出支通过轴突再生能够长入自主神经的副交感性纤维，并具有良好的传导运动兴奋的功能；利用截瘫平面以下健存的体反射，通过硬膜囊内神经根吻合的方法，可建立人工膀胱反射弧，实现患者的控制性排尿。

Each patient had more than two somatic symptoms in the two groups and more than 81.0％ of them had three somatic symptoms. METHODS: According to the different primary diseases in patients of the two groups, regular treatment was given routinely. Oral thymoleptics fluoxetine of 20 mg/d 5-hydroxyltryptamine reuptake restrainer with 20 mg/d was administered, the dose of which could be added to 40 mg/d according to the pathogenetic conditions.

根据两组患者不同原发病，遵循内科疾病治疗常规，予以正规治疗，同时予口服5-羟色胺再摄取抑制剂抗抑郁剂氟西汀，20 mg/d，根据病情可于2周后酌情加量至40mg/d。

Of the tested plants had the same fragment at all stages of PMCs and in somatic cells, but other 44% had not, among them, however, 0.76% of their anthers produced the same specific fragment only at synizesis stage, but not at other stages or in somatic cells.

在这一片段中设计特异引物再次对兰州百合DNA进行检测，56％的个体整体存在B1片段；在剩余的44％个体中得到了0.76％的花药特异片段检出率，时期为凝线期。

Based on the construction of international financial centre, we have pushed forward the explorations in the integration of cultural industries with financial services.

2010年及整个"十二五"期间，上海文化产业面临更有利的发展时机、更坚实的发展基础和更完善的发展环境。

The koala is an animal with a large bag in its body.

树袋熊用它的身体的一个大的袋子是一只动物。