查询词典 sequencing

The Cre gene was cloned from lysogen BM25.8 by PCR. The Cre gene was then inserted in between the CaMV35S-35S double promtor and Nos terminator of PBI525 plasmid after sequencing. The expression box was cut down and inserted into plasmid pBINPLUS to obtain the final fertility-restoring expression vector pBINPLUSCre.

PCR方法从溶原菌BM25.8基因组DNA扩增出Cre基因，克隆测序验证无误后插入PBI525 CaMV35S-35S双启动子和Nos终止子之间，再将表达盒切下插入pBINPLUS质粒相应位点，得到恢复基因植物表达载体pBINPLUSCre。

A synthetic model of multi-level production planning and control integration is brought forward, which integrated Master Production Schedule , Material Requirements Planning , Shop Floor Control , order acceptance control, order release control and process control into a whole. Besides, a new method of production management—— Theory of Constraints is introduced into the traditional MRP, and a method to integrate TOC with MRP is put forward. As a result, a system of production planning management for ceramics enterprise is developed. Then several problems of shop planning and control for ceramics enterprise including the modeling of production line, the optimized utilization of kiln, the complement and due-date of products are discussed. A mathematical programming model of equalization production for ceramics production line is set up. An optimized model is put forward, which uses genetic algorithm to solve the sequencing problem of ceramics before the kiln.

以生产计划与控制的集成为目标，提出将一个企业中的主生产计划、物料需求计划、车间作业计划与接受订单控制、投料控制与生产控制集成的多级生产计划与控制的总体模型，将一种新的生产管理方法——约束理论引入传统的MRP中，提出了实现TOC和MRP集成的具体方法；针对陶瓷企业的特点，为重庆兆瓷研制了一套陶瓷企业生产计划管理系统；并对陶瓷企业车间计划与控制中的一些具体问题进行了研究，建立了陶瓷生产线均衡生产的数学规划模型，就窑炉的优化利用、产品配套和交货期问题、陶瓷生产线的能力平衡等问题进行了研究。

Falciparum isolates prepared directly from 5 cases of cerebral malaria patients'blood in Mengla County, Yunnan Province and in Yingjiang County, Yunnan Province were used for polymerase chain reaction amplification and the two pairs of oligonucleotides for the highly conserved genes encoding the regions 12—17 in MAD20 merozoite surface protein 1(MSP1) of Papua New Guinea strain of P . falciparum were used as primers. The PCR products were digested with EcoRI and KpnI, respectively, and the generated fragment regions 16—17 were cloned into M13mp18 and M13mp19 vectors and their DNA were analyzed as the templates for DNA sequencing by the dideoxy chain-termination method.

应用多聚酶链反应技术对中国5 例脑型疟患者恶性疟原虫云南省勐腊县勐罕CMH/YN 分离株和云南省盈江县农场CYJ/YN 分离株基因组DNA 裂殖子表面蛋白1 (MSP1)第13—17 区基因进行扩增，将扩增产物分别经EcoRI 和KpnI 双酶切后，回收的MSP1 第16—17 区基因分子定向克隆M13mp18 和M13mp19 载体，按Sanger 双脱氧链终止法进行DNA 序列测定，并与MAD20、K1 和Wellcome 株原型基因进行同源性分析比较。

Methods Genomic DNA samples of two isolated Plasmodium falciparum isolate strains prepared directly from 5 cases of cerebral malaria patients′ blood in mengla County, Yunnan Province and in Yingjiang County, Yunnan Province were used for polymerase chain reaction amplification and the two pairs of oligonucleotides for the highly conserved genes encoding FC27 merozoite surface protein 2 (MSP2) of Papua New Guinea strain of Plasmodium falciparum were used as primers. The PCR products were digested with BamH1 and Hind Ⅲ respectively, and the generated fragment MSP2 were cloned into M13mp18 and M13mp19 vectors and their DNA was analyzed as the templates for DNA sequencing by the dideoxy chain-termination method.

应用多聚酶链反应对5例中国脑型疟患者恶性疟原虫云南省勐腊县勐罕分离株和云南省盈江县农场分离株基因组裂殖子表面蛋白2(MSP2)基因进行扩增，将扩增产物分别经BamHI和Hind Ⅲ双酶切后，回收的MSP2基因分子定向克隆M13mp18和M13mp19载体，按Sanger双脱氧链终止法进行DNA序列测定，并与恶性疟原虫株FC27、K1、IC1和CAMP株进行同源性分析比较。

Our patient evidenced a wide range of misidentification problems which mainly consisted of misidentifying place, person, and event, and these problems seem to be associated with deficits of temporal sequencing of memory, visual-spatial and visual perception, executive function, and awareness.

这位病患呈现多种层面的错认症状，这些症状可能与病患的时间顺序记忆、视空间知觉、执行功能与缺乏病识感等，多重认知功能缺损有关。

To realize effective operation of mixed assembly line,mixed sequencing problem was studied.

为使混合装配线有效运作，研究了混合装配线的生产排序问题。

In this study, a stress induction expression promoter of monocotyledon (P-WCS120) was amplified from wheat genome. The result of sequencing displayed a base substitution mutant of 1 bp and an insertion mutant of 21 bps. This sequence was registered at GENEBANK as a new found sequence, because 3% of the sequence was found to be non-homologous with the original sequence.

本研究从小麦基因组扩增单子叶植物逆境诱导表达启动子P-WCS120，测序证明包括1个碱基的替代突变和21个碱基的插入突变，与原序列有3%的非同源性，被GENBANK认定为新发现序列，现已登记注册。

Cloning and sequencing study of human bone morphogenetic protein 7 mature peptide ［J］.

人骨形成蛋白7成熟肽cDNA的克隆和序列测定［J］。

Cloning and sequencing study of human bone morphogenetic protein????

人骨形成蛋白7成熟肽cDNA的克隆和序列测定［J］。

Objective To analyse the cloning and sequencing of mature fragment of human bone morphogenetic protein－4 gene.

目的研究人骨形态发生蛋白4(2b)(BMP-4)成熟区cDNA的克隆方法，并进行测序及序列分析。

Objective:To compare the response control and attention of Schizophrenic patients with that of the healthy controls by Integrated Visual and Auditorycontinuous performance test. To quantitate the impairment of cognitive function in patients, and to explore the relationship between cognitive function and the severity of the disorder.

目的：探讨精神分裂症病人在IVA持续操作测试中的反应控制能力、注意力等，并与健康人进行比较，从而量化精神分裂症病人的认知功能损害，并进一步探讨其认知功能与疾病严重程度的关系。

Main effective factors including subcooling degree, mechanical vibration, gas hydrate reformation, environment temperature, noncondensing gas and surfactant are analyzed.

指出过冷度、机械振动、重复生成水合物、环境温度、不凝性气体、添加剂是影响气体水合物生成的主要因素，还对R152a水合物的放冷进行了实验研究。