查询词典 sequencing

Four groups of oligonucleotides were successively cloned in pMD18-T vector to obtain four large fragments. Ligation of these four fragments resulted in a cloned DNA MgE717 coding for the 29～268 amino acid region of gE. Sequencing result showed that there is a mutation at 255 amino acid, Asn instead of Asp.

分四段分别合成，基因拼接得到了改造后的基因片段MgE717，经测序，除了在gE基因+763位核苷酸处存在一碱基置换，导致第255位的氨基酸残基由Asp突变为Asn，其余的序列与PrV鄂A株gE基因的氨基酸序列相符。

Results A 357 bp DNA band was seen in the 2% agarose gel after the PCR product was electrophoresed. After the 357 bp DNA band was recovered and ligated into pMD-18T, human transforming growth factor-beta 3 encoding gene acquired through four-cycle PCR was confirmed by sequencing.

结果：4次PCR后，经2％的琼脂糖凝胶电泳可见一357 bp的条带，将电泳产物回收，连接入pMD-18T载体，经测序分析证实，所获得DNA片段为人转化生长因子β3的编码基因。

Finally we need an sequencing schema that both allow random insertion at arbitrary positions and rarely need to change saved data. Then this class come into idea inspired by fractional arithmetic of the positional number system.

因为采集信息不足，所以只要是人不同或者被看待的事情不同，总结出来的规律就不会完全一样，在地域和时间上再存在差距的话，这种不同就会更加剧烈，甚至能够导致完全格格不入的价值观念体系。

The cloning and sequencing of the full-length cDNA will lead directly to the discovery of novel genes.

新基因的研究在方法学上业已取得了很大的进步。

Found a different cDNA library with Malasesiza furfur in hypha phase by Subtractive Hybridization; And sequencing some fragment of differential expression gene. Provid foundation for the further study on complete differential gene expression.

采用消减杂交技术建立了糠秕马拉色菌的差异cDNA文库；并对部分克隆片段进行测序，为今后获得完整的差异表达基因打下基础。

First, to isolate the total RNA both in hypha and yeast phase of Malasesiza furfur, Then reverse them to cDNA and found a different cDNA library with Malasesiza furfur in hypha phase by Subtractive Hybridization; At last, clone and sequencing the gene fragment on differential expression of Malasesiza furfur in hypha phase.

首先提取糠秕马拉色菌两相细胞的总RNA；然后逆转录生成两相细胞cDNA，运用消减杂交技术获得菌丝相细胞差异表达的cDNA，并建立差异文库：最后对差异cDNA片段克隆测序。

The mitochondrial DNA cytochrome b gene sequences of 13 samples of gayal in China were sequenced by the method of direct sequencing and molecular phylogenetic tree was constructed based on cited sequences to analyzing its origin and phylogenetic status.

采用PCR直接测序法测定我国大额牛13个个体的线粒体Cyt b基因全序列，并引用其它牛种的序列资料共同构建分子系统树，以探讨大额牛的起源及其系统地位。

Sequencing revealed that the most common genes appearedin the library is photorespiratory-related genes, such as ribulose bisphosphate carboxylase, Rubisco activase, glyoxylate aminotransferase, glutamine synthetase,glyceraldehyde dehydrogenase, ferredoxin, transport protein, etc.

测序之后发现最为显著的是一些光呼吸相关的基因如核酮糖-1，5-二磷酸羧化／加氧酶、Rubisco激活酶、乙醛酸转氨酶、谷氨酸转氨酶、甘油醛脱氢酶、铁氧还原蛋白和转运蛋白等。

This richly illustrated expanded edition of Looking In: Robert Frank's "The Americans" contains several engaging essays by curator Sarah Greenough that explore the roots of this seminal book, Frank's travels on a Guggenheim fellowship, the sequencing of The Americans and the book's impact on his later career.

这说明寻找丰富的扩展版：罗伯特弗兰克的"美国人"，包含若干文章，搞馆长莎拉格里诺的探讨这一重要著作，在古根海姆奖学金弗兰克旅行的根源，美国人的排序和这本书的影响，他后来的职业生涯。

This richly illustrated expanded edition ofLooking In: Robert Frank's "The Americans"contains several engaging essays by curator Sarah Greenough that explore the roots of this seminal book, Frank's travels on a Guggenheim fellowship, the sequencing ofThe Americansand the book's impact on his later career.

这说明了丰富的扩展版ofLooking在：罗伯特弗兰克的"美国人"，包含若干文章，搞馆长莎拉格里诺的探讨这一重要著作，在古根海姆奖学金弗兰克旅行的根源时，Americansand本书对他后来的职业生涯的影响测序ofThe。

Cynanchum Lingtai apricot production in the average weight 65 grams, the brightly-colored fruit, juicy rich, sweet-sour taste, sweet from the nucleolus, when the late Qing Dynasty famous Shaanxi, Gansu provinces, the Qing imperial court Tongzhi tribute for years.

灵台生产的牛心杏平均单果重65克，果实色泽鲜艳，汁多味浓，甜酸适口，离核仁甜，清末时就驰名陕、甘两省，清同治年间曾为朝廷贡品。

Chenopodium album,Solanum nigrum, and Amaranthus retroflexus were very susceptible to the herbicides. Polygonum persicaria and Abutilon theophrasti were relatively less susceptible to the herbicides, and Lycopersicon esculentum was not susceptible to it. The relationship between reduction rates of weed biomass and PPM values of weed leaves 2,4, and 6 days after treatment was established.

供试的6种杂草对该混剂的敏感性存在显著差异：红心藜Chenopodium album、龙葵Solanum nigrum和反枝苋Amaranthus retroflexus对该混剂最敏感，ED90值分别为47.65、71.67和29.17g/hm2；春蓼Polygonum persicaria和苘麻Abutilon theophrasti敏感，ED90值分别为96.91、114.20g/hm2；而番茄不敏感。