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Serum samples should be collected instead of plasma samples from anticoagulants heparin and trisodium citrate when copies of HBV DNA were detected. Plasma samples from anticoagulant dipotassium EDTA is also used with quantitative assays for HBV DNA.

实时荧光定量PCR技术检测乙型肝炎病毒DNA拷贝数，应避免使用含肝素钠和枸缘酸钠的血浆标本，使用不含抗凝剂或含EDTA-K2抗凝剂的无菌试管收集标本。

Results the orifices of dentinal tubule were mostly occluded in Gluma samples and partly occluded in Bisblock samples. Resin layer were observed in Single Bond 2 samples.

结果 Gluma组牙本质小管开口几乎堵塞，Single Bond 2组牙本质表面形成厚的树脂层，BisBlock组牙本质小管开口部分堵塞。

The positive rate of nucleocapsid was 71.43% in pharynx samples, 66.67% in stool samples, and 26.67% in herpetic liquid samples.

手足口病是小儿常见传染病，绝大多数为轻型病例，预防的重点是高发期健康教育。

The proportions of Ⅰ~Ⅱgrade and Ⅲ grade were 73.8%、 83.1%; 45.7%、 65.2% between 92 samples of Urgur patients and 188 samples of han patients . Lymphy node metastasis in Uygur patients were greater than Han patients in Ⅰ~Ⅱgrade and Ⅲ grade (X~2=13.14, P.001; X~2=5.08, P.05).(3) The proportions of the expression of VEGF-AmRNA and its protein were 31.8%、 57.1%、64.3%, 27.3%、 53.6%、 59.5% in difference pathological grade in 92 samples, With increasing of the pathological grade,the VEGF-AmRNA and its protein expression are highly (X~2=6.26, P.05; X~2=6.21, P.05); The proportions of the positive expression of VEGF-AmRNA and its protein were 68.4

392例浸润性乳腺癌组织中VEGF-AmRNA和其蛋白的表达在不同组织学分级中其阳性率分别是31.8%、57.1%、64.3%，27.3%、53.6%、59.5%；二者在不同组织学分级中表达差异具有统计学意义(X~2=6.26，P.05；X~2=6.21，P.05)；在淋巴结转移组与非转移组中VEGF-AmRNA和其蛋白的表达阳性率的分别是68.4%、31.49，61.4%、31.4%，在淋巴结转移组VEGF-AmRNA和蛋白的明显高于淋巴结未转移组(X~2=11.96，P.001；X~2=7.79，P.05)；VEGF-AmRNA和其蛋白的表达

Two virus samples were collected from Malvastrum coromandelianum and these samples were tested by PCR. The results showed that a specific band about 500 bp was amplified from the samples Fz1 and Fs1 with degenerate primers PA and PB.

PCR产物测序进行序列比较发现：2组赛葵样本上病毒之间的差异极小，核苷酸序列同源性大约为96％，因此推测：这两组赛葵样本可能是由(来源：Af3BC7bab论文网www.abclunwen.com)同一种病毒侵染。

Detection results showed samples VA2, VA7 and VA8 from Malvastrum coromandelianum had weak reaction with MAb SCR18, and samples X2 from Siegesbeckia orientalis had weak reaction with MAb SCR18 and SCR106, while other samples had no reaction with MAbs.

用双生病毒兼并引物PA、PB对杂草样本进行特异性的PCR检测，结果发现仅有赛葵样本VA2、VA7、VA8和稀硷样本X2出现约500bp条带，说明这些杂草上存在双生病毒。

These suggest that the xenocrysts were captured by the enclave magma from the host magma. On diagram of total alkali vs. SiO〓(Middlemost, 1994), most samples of the host rocks plot within the area of diorite, the samples of the quenched margin plot within the area of gabbro-diorite and the samples of the enclave plot within the area of diorite.

在Middlemost(1944)提出的侵入岩全碱-SiO〓分类图解中，寄主岩的投影点落在花岗闪长岩范围内，淬冷边的投影点落在辉长闪长岩范围内，而包体的投影点大都落在闪长岩区。

Moreover, the actual allele frequency of most varieties deviates far from Hardy-Weinberg equilibrium. All PPB、na 、I、h、Gi and Fst have proved to be the references to elucidate that ISSR is a most powerful tool to analyze genetic diversity, compared with the RAPD marker and the allozyme marker is less strong ordinally. We could divided the 70 samples into A, B, C, D and E five groups using three methods according to genetic distance clustering. There is a bit displacement for few varieties in different clustering maps, but the most are similar to morphological analysis despite that there is still a great difference among cultivars in the same one group. The above results imply that the three methods have the different sensitivity and resolution in genetic distance analysis of close varieties. The Mantel test indicates that the results from the three kinds of markers have the significant correlation, which demonstrates that the number of the used three kinds of markers is enough to exactly detect the diversity of all 70 samples to ideal extent. And these methods can be used to evaluate the diversity of the whole group using the miscellaneous samples instead of the individual sample, of the Gerbera jamesonii are mainly from tissue culture plants. In conclusion, the above study results provide a reference for the application of three kinds of molecular markers to molecular marker-assisted breeding of flower. 2. The genetic diversity among the eight introduced cut-flower varieties of Ranunculus asiatica was analyzed by the ISSR markers. Based on the genetic clustering tree, all the colorful flower varieties are clustered into one group, and the white flower varieties into another group. Moreover, among the former group the yellow flower varieties are clustered into one sub-group, and the reddish flower varieties, such as rose color, pink, nacarat, are clusetered into another sub-group.

由三种分子标记的分析结果可以看出，等位基因平均值、观察杂合度、Fis值、Fit值皆较高，表明非洲菊等位基因较丰富，杂合基因偏多，且绝大部份品种的实际等位基因频率在品种内偏离了Hardy-Weinberg equilibrium；PP8、na、Ⅰ、h、Gi及Fst皆表明，ISSR检测遗传多样性的能力最强，其次是RAPD，等位酶最低；根据遗传距离进行聚类，三种方法都把70个品种分成A、B、C、D、E五个大组，每一组中除少数品种发生位移外，大部份品种分类结果相似，且与形态分析结果有相似性，但在每一组中，品种间的聚类差别较大，表明这三种方法在近距离品种间检测遗传变异时灵敏度及分辨力不同；Mantel检测表明，三种标记的分析结果有显著相关性，表明所用的三种分子方法的标记数量已经可以相对无偏地检测到70个品种间遗传变异；非洲菊为组培苗，三种标记的检测结果皆表明，混合样品可以作为个体样品的代表，对整个居群的遗传多样性进行评价；这些研究结果可为三种分子标记方法在花卉分子辅助育种中的进一步应用提供借鉴。

The testing methods and targets were shown as below:Recorded milk weights from the 7th day postpartum to 15th day postpartum,and calculate the average day-milk yield;Milk samples were draw in 7th day and 14th day postpartum,and testing the milk fat,milk lactose,milk protein,milk Zn and the milk somatic cell counts;Blood samples were adopted at the 7th day postpartum,testing the levels of serum PRL; Blood samples adopted at the 1st day and the 12th day in oestrous cycle were used to test the levels of E_2 and P_4 in serum;In the experimental stage, registering the condition of parturition, the mean birth weight of calves,the first oestrus,the oestrus scycle postpartum and the conception rate of the first service after artificial insemination.

试验方法及所测指标如下：于产后第七天到第十四天记录日产奶量，最后计算每组平均日产奶量；每组随机抽取分娩后第七天、第十四天的奶样，测定乳糖、乳蛋白、乳脂、乳锌含量及乳体细胞数；于产后第七天，随机采取奶牛血样，测定血清中PRL含量；于发情周期的当天和第12天，随机采取血样，测定血清中雌二醇(Estradiol，E2)和孕酮(Progesterone，P)的浓度；记录奶牛的分娩情况、犊牛初生重、产后第一次发情日期、产后发情周期，并采用人工授精技术进行受精，记录第一情期受胎率，最后取平均值，作为衡量奶牛繁殖性能的指标。

Under optical microscope, the observational result indicates that the peach blossom stone samples from Gao Mountain contains transparent and distransparent minerals which forms pealike and red spots. The XRD testing result indicates that the mineral compositions in the samples are dickite, hematite, quartz and a little pyrophyllite. LRM testing result shows that the basic part of the samples is dickite, the red minerals with both well crystal shape and dip dye shape are hematite, and colourless and transparent mineral grains are quartz.

光学显微镜的观察结果表明，高山桃花石样品中存在透明和不透明矿物，其中不透明矿物形成了艳丽的红色斑点；XRD测试表明，样品的矿物组成为地开石、赤铁矿、石英以及少量的叶腊石；拉曼光谱测试结果表明，样品的基质部分为地开石，结晶形态完好的红色矿物颗粒与呈浸染状的红色部分均为赤铁矿，无色透明的矿物颗粒为石英。

Cynanchum Lingtai apricot production in the average weight 65 grams, the brightly-colored fruit, juicy rich, sweet-sour taste, sweet from the nucleolus, when the late Qing Dynasty famous Shaanxi, Gansu provinces, the Qing imperial court Tongzhi tribute for years.

灵台生产的牛心杏平均单果重65克，果实色泽鲜艳，汁多味浓，甜酸适口，离核仁甜，清末时就驰名陕、甘两省，清同治年间曾为朝廷贡品。

Chenopodium album,Solanum nigrum, and Amaranthus retroflexus were very susceptible to the herbicides. Polygonum persicaria and Abutilon theophrasti were relatively less susceptible to the herbicides, and Lycopersicon esculentum was not susceptible to it. The relationship between reduction rates of weed biomass and PPM values of weed leaves 2,4, and 6 days after treatment was established.

供试的6种杂草对该混剂的敏感性存在显著差异：红心藜Chenopodium album、龙葵Solanum nigrum和反枝苋Amaranthus retroflexus对该混剂最敏感，ED90值分别为47.65、71.67和29.17g/hm2；春蓼Polygonum persicaria和苘麻Abutilon theophrasti敏感，ED90值分别为96.91、114.20g/hm2；而番茄不敏感。