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Results 371 bp DNA fragment was amplified from 24 different species. The sensitivity could be improved to 10-12 g. No signal was observed when human DNA,funguses and viruses were used as templates. 22 blood samples and 4 cerebrospinal fluid samples, being positive with culture, were positive by using PCR. The gramnegative and grampositive probes hybridized to clinic samples and different species, as predicted by Gram stain characteristics.

结果 对24株不同标准菌株进行PCR扩增,均出现371 bp长度的DNA片段,敏感性试验可检测出10-12 g的细菌DNA,与人类基因组DNA、真菌及病毒无交叉反应;22例血培养阳性标本及4例脑脊液培养阳性标本均扩增出371 bp长度DNA条带,反相杂交法区分革兰阳性/阴性细菌与培养结果相符。

In this paper, a total of 56 relatively nonpoint source pollution sediment samples were collected from the tidal flats of the Jiuduansha shoal and the Chongming island of the Yangtze river estuary. The main properties i. e., grain size distribution, contents of DCB (dithionite citrate bicarbonate extracted iron Fe(subscript d, AAO extracted iron Fe(subscript o, total Fe, DCB extracted Mn Mn(subscript d, total Mn, organic matter, magnetic susceptibility of the samples, as well as the concentrations of Cu, Zn, Pb, Cr, Cd and Ni in the samples, were determined. Basing on the data, the relation of the sediment properties to heavy metal concentrations was evaluated with pearson's rank and partial rank correlation analysis.

采集长江口无明显点源污染的崇明东滩和九段沙潮滩56个沉积物样品,测定沉积物原样和粘粒中Cu,Zn,Pb,Cr,Cd和Ni的含量以及沉积物的主要性状(粒径分布、游离铁、活性铁、总铁、游离锰、总锰、有机碳和磁化率等),以相关分析和偏相关分析研究沉积物中重金属含量与沉积物主要性质之间的关系。

There is not a significant difference in these groups by one-way analysis of variance. 2 Among 51 cases with lung cancer, 7 specimens showed malignant cells in bronchial lavages, including 4 cases in central lung cancer and 3 cases in peripheral lung cancer. Concordant results were observed in 20 cases. 6 specimens showed malignant cells on cytological analysis, and NMSP was positive in at least one gene tested. 14 samples did not show malignant cells on cytological analysis, and the NMSP results were correspondingly negative. The results of cytology and NMSP were discordant in 31 samples. 30 samples were cytologically negative, but NMSP positive in one or more genes. In addition, there was one case that the cytology was positive for malignant cells, but the NMSP was negative in all of the three gene tested.

51例肺癌患者支气管灌洗液细胞学检查发现肺癌细胞7例,其中27例中心型肺癌患者4例支气管灌洗液发现肺癌细胞,24例周围型肺癌患者3例支气管灌洗液发现肺癌细胞。20例支气管灌洗液细胞学检查和过甲基化检测得到一致结论。6例支气管灌洗液发现肿瘤细胞,过甲基化分析至少一个基因阳性。14例灌洗液未发现肿瘤细胞,三个基因过甲基化分析均为阴性。31例支气管灌洗液细胞学检查和过甲基化检测得到不一致结论,30例细胞学检查为阴性,但过甲基化分析一个或一个以上基因为阳性。1例细胞学检查阳性但过甲基化分析阴性。

Analyses of a neighbor-joining tree of identified DNA haplotypes and F values between samples, and an analysis of molecular variance revealed significant genetic differences between samples from Portugal and Taiwan, but no differentiation among the samples from Taiwan. These results suggested that the common mackerels off Taiwan belong to the single gene pool.

单基因型之neighbor-joining树、族群样本间之FST值及分子变方等分析显示葡萄牙及台湾之三族群样本间皆有显著之遗传差异,但台湾之三族群样本间并无显著差异;此结果指出台湾附近之白腹鲭属於单-基因库。

The prewashed samples and postwashed samples were analyzed by seminal fluid assay.RESULTS:(1) The positive rate of semen HBV DNA was 21.05%(12/57) with 4.7810~4copies/ml on average; the positive rate of serum HBV DNA was 81.82%(45/55 with 2.3310~7copies/ml on average. There is positive rank correlation between semen HBV DNA copies and serum HBV DNA copies;(2 There were 7 positive cases in 21 samples before washing, the 6 cases except one were positive after washing;(3) there arepositive rank correlation between viability、round cells、 abnormal morphology and semen HBV DNA copies.

结果:(1)精液HBV DNA阳性检出率为21.05%(12/57例),平均拷贝数为4.7810~4/ml,血清HBV DNA阳性检出率为81.82%(45/55例),平均拷贝数为2.33(10~7/ml,精液与血清HBV DNA拷贝数等级间存在正相关关系;(2)21份用于洗涤的精液标本中,7份精液HBV DNA阳性,阳性率为33.33%(7/21例),洗涤后仍有6份标本阳性;(3)精液常规分析显示精子活力、圆细胞数、精子畸形率与精液中HBV DNA载量之间存在正相关关系。

Front-peak dominated double peak mode and double peak group mode were mainly found in the rain-particle samples, while the front-peaked dominated double peak group mode is mainly found in the rain-particle samples ofdry-season, and double peak group mode is mainly found in the rain-particle samples of wet-season.

正构烷烃分布模式呈现一定季节变化,干季雨水主要为单峰态模式分布,而平台型模式主要出现在春季的雨水中,夏季雨水则三种模式都有;干季雨水颗粒物主要为双峰态前峰型模式,而湿季则以要双峰态双峰型模式为主。

Methods 34933 serum samples were screened by ELISA method on Treponema pallidum antibody. The samples with probable positive result were retested by toluidine red unheated serum test and Treponema pallidum particle assay. Results There were 559 serum samples with probable positive result.

对34933例标本采用酶联免疫吸附试验进行梅毒螺旋体的初筛,初筛结果为可疑阳性的样本进行梅毒螺旋体明胶凝集试验和甲苯胺红不加热血清反应素试验的检测。

Initially in the trade company to do the domestic Merchandiser, the company produced for the Japan and South Korea customer service hat, gloves, scarves and other products made from the customer sampling for plant samples, and samples sent to clients on satisfaction after the inquiry after the quotation; the next single post-production samples to confirm large cargo up to date feedback to the factory; ship sailing arrangements and related information before the production to the shipment after the completion of data archiving easy to find.

最初是在贸易公司做国内跟单员,公司为日韩客户服务制作帽子、手套、围巾等产品,从客户打样寻找工厂制作样品,并将样品寄到客户处后询问满意度后报价;下单后确认大货生产样品及时更新反馈至工厂;出货前船期安排与相关资料的制作到出货完成资料归档便于以后查找。

The basic mechanical performance of perforated shale brick masonry is studied by analyzing the experimental results of compression strength of 25 samples of the masonry ,bending tensile strength of 14 samples and shearing strength along straight joint section of 45 samples.

笔者通过对多孔页岩砖砌体25个试件的抗压强度、14个试件的弯曲抗拉强度和45个试件的沿通缝截面抗剪强度的试验结果进行分析,研究了多孔页岩砖砌体的基本力学性能,对其在设计和使用中的部分技术问题进行了探讨。

The 29 F globular clusters in the galaxy are selected as samples in my paper (The space distribution and motional orbits of the samples have not been researched in detail before.).According to the basic parameters: azimuth coordinates, distances from the sun, radial velocities and proper motions of sample clusters, the initial positions and velocities of the samples are reduced using the galactic coordinates, and their orbits are integrated by numerical method for three different galactic gravitational potential models.

本文选用银河系中29个累积光谱型为F型的球状星团作为样本(这些样本星团的空间分布和运动特征都没有被详细地讨论过),根据它们的基本资料:方位坐标、日心距、视向速度,绝对自行等参数,归算处理得出了各样本星团的空间分布和运动速度。

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Cynanchum Lingtai apricot production in the average weight 65 grams, the brightly-colored fruit, juicy rich, sweet-sour taste, sweet from the nucleolus, when the late Qing Dynasty famous Shaanxi, Gansu provinces, the Qing imperial court Tongzhi tribute for years.

灵台生产的牛心杏平均单果重65克,果实色泽鲜艳,汁多味浓,甜酸适口,离核仁甜,清末时就驰名陕、甘两省,清同治年间曾为朝廷贡品。

Chenopodium album,Solanum nigrum, and Amaranthus retroflexus were very susceptible to the herbicides. Polygonum persicaria and Abutilon theophrasti were relatively less susceptible to the herbicides, and Lycopersicon esculentum was not susceptible to it. The relationship between reduction rates of weed biomass and PPM values of weed leaves 2,4, and 6 days after treatment was established.

供试的6种杂草对该混剂的敏感性存在显著差异:红心藜Chenopodium album、龙葵Solanum nigrum和反枝苋Amaranthus retroflexus对该混剂最敏感,ED90值分别为47.65、71.67和29.17g/hm2;春蓼Polygonum persicaria和苘麻Abutilon theophrasti敏感,ED90值分别为96.91、114.20g/hm2;而番茄不敏感。

However, I have an idea.

不过,我有个主意。