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After amplying a 2.2kb fragment form the PPV-SC1 RF-DNA,we clone the fragment into pMD 18-T,named pTNSl.The whole sequence which is 1989 bp long was determined by sequencing, including the complete ORF of PPV-SC1 NS1 which encoding 662 amino acids.Alignment of pairs of sequence indicates that there are 98% and 99% similary with other porcine parvovirus strains Kresse and NADL-2, respectively. Multiple sequence alignment discloses that there are a few difference between ppv-scl nsl gene and other ppv nsl gene: A-G at 39nt,T-C at 153nt,A-G at 175nt, A-C at 1117nt, A-C at 1535nt .Alternative codon in ppv-scl nsl have distinctly different frequentfy by codonbias analysis at EMBOSS(http://genopole.toulouse.inra.fr/bioinfo/emboss). Thereis not distinct hydrophobicity and transmenbrane helices in ppv-scl nsl protein. Struction domain anslysis of PPV-SC1 NS1 protein indicate that there are a ATP/GTP-binding site motif A at 398-405,16 Protein kinase C phosphorylation site,21 Casein kinase II phosphorylation site,and 3 cAMP/cGMP-dependent protein kinase phosphorylation site.At the same time ,there is a same motif between ppv-scl nsl and Poxvirus D5 protein-like which may share in the same fuction which is necessary during virion duplication.

将PPV-SC1 NS1序列与其他PPV NS1基因进行多序列比对，结果显示，PPV-SC1 NS1与其他的PPV NS1的同源性较高，仅存在个别的差异，分别是第39位A→G，第153位T→C，第175位A→G，第1117位A→C，第1535位A→C；同源搜索比较表明，PPV-SC1与PPV NS1同源性可达98％、99％，与其他的细小病毒NS1基因也存在很大的保守性；密码子偏向性分析结果表明PPV-SC1 NS1基因在同一氨基酸的不同密码子的选择上存在一定的偏向性；PPV-SC1 NS1蛋白总体上说具有亲水性不存在明显的疏水性区段，用swiss TMPRED软件预测PPV-SC1 NS1的跨膜区，返回的结果并没有得到有显著意义的跨膜区的存在；根据基于motif数据库的结构域预测，PPV-SC1 NS1的第393-415位氨基酸残基存在潜在的ATP／GTP结合位点，该蛋白还存在16个蛋白激酶C磷酸化位点，21个酪蛋白激酶2磷酸化位点，3个cAMP-／cGMP依赖蛋白激酶磷酸化位点，PPV-SC1 NS1蛋白与POX_D5(痘病毒D5蛋白)具有一致的保守结构域，推测NS1可能与POX_D5有类似的功能。

He wants to see the same faces, to read the same books, to do the same things day after day, month after month, while he is writing, so that nothing may break the illusion in which he is living--so that nothing may disturb or disquiet the mysterious nosings about, feelings round, darts, dashes, and sudden discoveries of that very shy and illusive spirit, the imagination.

他希望面对相同的人，读相同的的书，日复一日年复一年做着相同的事情，当他写作时，就没有任何事可以打破他生活在其中的幻觉了——打破或扰乱他对周围事物神秘的嗅觉与感知，那些急冲，猛击，然后突然发觉的微妙的幻想世界里的精神，那些想象。

The same type of wine, the same grape from the same region may differ vastly depending on the wine maker.

所以，同样型态的葡萄酒，相同的品种，同样的产区都会因为酿酒师的不同而有天壤之别的表现。

It helps that they are from the same 40-something generation of politicians, that they share the same privileged upbringing and liking for well-tailored suits, and that they have the same instincts on civil liberties and individual freedom.

他们同属40岁左右的这代政界人士，成长背景都很优越，都喜欢剪裁考究的西服，都发自内心的追求民权和个人自由--这些都有帮助。

Both starting torque and running torque can be measured with the same system, the same tooling and in the same test cycle.

这两种起动转矩和运行转矩可以测量同一系统，同一工具和在同一检验周期。

Tom and Ted lived in the same street, walked into town every morning at the same hour, dined at the same slap-bang ''.

汤姆和特德住在同一条街上，每天早晨同时走到城里市区，每天在同一个小饭馆进餐。

The results show that with the increase of strain rate, the grain sizes of thermal simulation AZ31B alloys with different deformation all decrease; however, when other conditions are the same, the higher deformation will lead to the formation of the smaller grain size, and as the cooling intensity decreases, typical cast structure of AZ31B will appear. With the decrease of roll casting temperature, the dendritic size of roll casting AZ31B alloy sheet prepared by the same strain rate is also reduced, but with the increase of strain rate, the dendritic size of the alloy sheet prepared by the same initial roll casting temperature is decreased, and the tendency of dendritic streamline is strengthened. During the roll casting process of AZ31B alloy, rolling force should not be large, otherwise it will cause heat cracking.

研究结果表明：在不同应变量下，热模拟样品的晶粒粒度均随应变速率的增加而减小，而当其他条件相同时，变形量越大晶粒粒度越小，冷却强度降低，合金呈典型铸态组织；利用铸轧技术生产的AZ31B合金，当应变速率一定时，随着初始铸轧温度的降低，铸轧态板材的树枝晶粒度逐渐减小；而在初始铸轧温度一定时，随着应变速率的增大，铸轧态合金板材的树枝晶粒度也逐渐减小；而随着应变速率的提高，树枝晶沿轧向呈流线状排列的趋势增强；在AZ31B合金铸轧过程中，轧制力不能太大，否则容易引起热裂。

Article 8 For national focal point shelter belt, and forest for special uses, the competent forestry authority of State Council shall produce opinions and report to the State Council for approval, then promulgate it; for local key shelter forest and forest for special use, the competent forestry authority of the people's government of a province, autonomous region or municipality directly under the central authority shall produce opinions and report to the people's government at the same level for approval, then promulgate it; for other shelter forest, timber forest or special-use forest as well as economic forest or fuel forest, the competent forestry authority of people's government at county level shall demarcate according to the state's relevant regulations on forest categorization and the arrangement and organization of the People's government at the same level, and report to the people's government at the same level for approval and promulgation.

第八条 国家重点防护林和特种用途林，由国务院林业主管部门提出意见，报国务院批准公布；地方重点防护林和特种用途林，由省、自治区、直辖市人民政府林业主管部门提出意见，报本级人民政府批准公布；其他防护林、用材林、特种用途林以及经济林、薪炭林，由县级人民政府林业主管部门根据国家关于林种划分的规定和本级人民政府的部署组织划定，报本级人民政府批准公布。

Such doubts havesitimulated the researchers'curiosity and start to explore the students' terms This research proceeds by observation interview test using learning sheets and etc The purpose is to explore students' terms of scientific concepts sources of terms connections between students' usage nomenclature and chooses of usage The results of the research are as follows: 1 There are five kinds of naming principles for students' terms of scientific concepts: appearance habit function replacing and sound Appearance principle contains three categories: color shape and partial construction; habit principle contains custom and ability; replacing principle contains reality and non-reality 2 Students' terms of scientific concepts come from two ways: self-acquisition and learning through senses The former way is only a bit more common than the latter 3 There are three different relationships between students' usage of terms and nomenclature: one both the same in usage and meaning; the other both different; another different usage but the same meaning Normally terms that students used are various from nomenclature in the aspects of usage and meaning only a few would match and even rare for "Synonyms" to occur 4 Alternative options for students' scientific terms are maintenance adaptation or co-existence with formal scientific terms The most common way of selecting usage of terms is to maintain Sometimes students would adapt themselves once learn of scientist's term However some believe that very odd chances for both could exist at the same time

这样的疑问深深引起研究者的好奇，著手探索学生科学概念所使用的语言。本研究透过观察、访谈、学习单施测等方法来进行，目的在探讨学童在科学概念上所使用之学生惯用语、学生惯用语来源、学生惯用语与科学家用语之关联性、学生惯用语选择。研究结果如下：一、学童在科学概念上所使用之学生惯用语的命名原则有五种：外型、习性、功能、藉代称呼及声音，其中外型原则有颜色、体型及部份构造三类目，习性原则有习惯及能力两类目，藉代称呼原则有实体及虚拟两类目。二、学童在科学概念上所使用之学生惯用语有两种来源，分别为自己称取和听来。学生惯用语来源较多为自己称取，透过其他方式听来少一点，但两种来源的比率差距甚小。三、学童在科学概念上所使用之学生惯用语和科学家用语的关系有三种，分别为用语意涵均相同、用语不同意涵相同和用语意涵均不同。学生惯用语通常和科学家的用语是不同的，且其意涵也不同，只有部份学生惯用语和科学家用语意涵均相同，至於学生惯用语和科学家用语不同但意涵相同的极少。四、学童的科学概念相关学生惯用语选择有三种，分别为维持学生惯用语、改变成科学家用语和科学家与学生惯用语皆可。学童的科学概念相关学生惯用语选择，以维持学生惯用语最多，而有些时候学生会在得知科学家的用语后改变成科学家用语，但认为科学家用语和自己使用的学生惯用语皆可的则很少。

I will not imprison members of the same party in the same cell block, let alone the same cell.

我不会将同一队伍的人都关在一起，而是各自囚禁。

For the head-teacher of the class said that I had seriously broken the school rules,which led me to a three-day suspend class.

为什么呢？因为我的班主任说我严重的违反了校规，于是让我停课三天。

Many of them believe that the conversion of thousands of working-class folk in England spared that nation from the mass carnage and the tyranny that came with the revolution in France.

他们之中有许多人相信，在英国数以千计的劳工阶级之悔改信主使英国免於遭受如法国大革命所造成的大屠杀和专制暴政。