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When reached logarithm growth phase, it was poured or the medium was removed. 5 mL medium containing 10 mg/L mitocin-C was added , kept at 37 ℃, and then cultured in saturated humidity warm box with the CO2 of 0.05 fraction volume for 2 or 3 hours. It was coated with 0.1% gelatin in 6-hole plate, laid for over 30 minutes, and then threw away or the medium with mitocin-C was removed, washed with phosptat buffer for 5 times so as to remove the mitocin-C. 2 mL 0.05% trypsin digestive cells were added, and it was observed under microscope. When crevice appeared, cells became round (about 2-4 minutes), digestion was stopped by adding medium of the same volume, and blew up with straws repetitively to make it into monoplast suspension. Special-used cover glass was put in the center of cell counting chamber. Cells were sucked in by glass siphon, and cell suspension flew out at bucket of up or down-sides of counting chamber, until the cover glass was filled with fluid. Living cell were inoculated at concentrations of 3×108, 5×108, 1×109 L-1 after their number counted.

选取2~5代的小鼠胚胎成纤维细胞,待其至对数生长期倒掉或吸掉培养基,加入含10 mg/L丝裂霉素C的细胞全培养液5 mL,置37 ℃,体积分数0.05的CO2饱和湿度温箱中培养二三小时,在6孔板中加入0.1%明胶包被,放置30 min以上,倒掉或吸掉含丝裂霉素C的培养基,磷酸盐缓冲液清洗5遍,尽量除去丝裂霉素C,加入2 mL 0.05%的胰蛋白酶消化细胞,镜下观察,当细胞间出现裂隙,细胞变圆时(约2~4 min),立即加入等量的全培养液终止消化,并用吸管反复吹打,使之成为单细胞悬液,在细胞计数板中央放置专用的盖玻片,用玻璃虹吸管吸取细胞,让虹吸管在盖玻片上或下侧的计数板凹槽处流出悬液,至盖玻片下被液体充满为止。

Ca-alginate immobilized Phanerochaete chrysosporium and Trametes versicolor removed 79.4% and 85% COD from bleaching wastewater at the seventh day, compared to 76% and 81% COD removed by free fungi. Hence, it is demonstrated that the microbial immobilization is highly feasible to treat the bleaching wastewater for discoloration and COD reduction.

而以海藻酸钙固定的白腐菌Phanerochaete chrysosporium 与Trametes versicolor,对制浆废水COD在第七天有79.4%及85%的去除率,高於同时间自由生长的菌体COD去除率76%及81%,因此本研究证明固定化微生物处理制浆废水有很高的可行性。

At 60 ℃ the reaction of 1-O-alkyl-2-O-acetyl-3-O-trityl glycerol with iodine/methanol system removed trityl and acetyl and gave 1-alkyl glycerol. But at r.t. the reaction only removed trityl and gave two isomers 1-O-alkyl-2-O-acetyl glycerol and 1-O-alkyl-3-O-acetyl glycerol.

1-烷氧基-2乙酰氧基-3-三苯甲氧基甘油醚与碘/甲醇体系反应,在60 ℃时,同时发生了脱三苯甲基及乙酰基,在室温下,仅脱三苯甲基,但乙酰基在反应过程中发生了部分转移,产生了两个异构体。

At first, mineral composition and distributing of impurities in andalusite grains were studied by SEM and EDS. The results showed that main mineral impurities were biotite, ilmenite, clay species, the wall rock conglutinated at brim of andalusite and impurities in the chiastolitic cruciform pattern were easily removed, but the small impurities in andalusite grain were hardly removed.

首先,利用SEM和EDS分析了红柱石矿的矿物组成和杂质分布,结果显示这种红柱石矿所含的杂质矿物主要是黑云母、钛铁矿和黏土矿物等,其中分布在红柱石颗粒边缘的围岩和空晶石内的黑十字容易去除,而分布在晶内的细粒杂质则不易去除。

In this study for characteristics of reducing Cr by SRB7, adsorption approach couldn't remove Cr; electron transfer approach which removed 51.42% of the Cr at 24 h, was not preponderant in the process of reduce Cr; sulfureted hydrogen approach which removed 78.02% of the Cr at 24 h, was preponderant in the process of reduce Cr.

在SRB7去除Cr的特性中,吸附途径对Cr的去除几乎不起作用;电子传递途径在Cr的还原过程中不占优势, 24 h去除率为51.42%;而H2S途径在Cr的还原过程中占主导地位, 24 h去除率为78.02%。

Cellulosic impurities such as burrs and vegetable fibers that were not removed during carding must be removed from wool cloth by chemical treatment before it can be further processed.

在梳理过程中没有除去的存留在毛织物中的纤维素类不纯物质,在织物进一步加工之前,必须通过化学方式清除掉。

Sand cores usually can be removed by shaking.sometimes they must be removed by dissolving the core binder.

砂芯通常可以去除摇晃。有时,他们必须拆除解散核心粘结剂。

G-type fixtures can be removed so an hour later, immediately after the leak can be removed to eliminate Gate Valves quickly on aluminum rivets to rivet hole.

G型夹具可等一小时后取下,亦可消除泄漏后马上取下,迅速用铝铆钉把钻孔铆上。

The pedal, lock, basket and back seat, ect which can lead to hurt must be removed, otherwise when checking they are removed and kept by the committee technician.

脚撑、锁、篮筐、后座等可能导致伤害的非必要配件请再赛前自行拆卸,否则将在检录前的车辆安全检修时由组委会技师拆除保管。

Sulfur Recovery. The main feed streams to the SRU (Fig. 2) are acid gas from the AGRU regeneration column, which contains all H2S removed from the feed gas and the acid gas from the regeneration-gas treatment unit that contains the mercaptans removed in the MSU.

硫磺回收:输至同步再生机组的主给原料气流(图2)是来自AGRU再生塔的酸性气体,这气体中所含有的硫化氢是从含有硫醇的再生气体解决机组的原料气和酸性气里脱离出来的。

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What are your goals and strategies for growth?

你的成长目标和策略是什么?

And unto the angel of the church in Sardis write; These things saith he that hath the seven irits of God, and the seven star I know thy works, that thou hast a name that thou livest, and art dead.

3:1 你要写信给撒狄教会的使者,说,那有神的七灵和七星的,说,我知道你的行为,按名你是活的,其实是死的。

"It is a testament to making sure unemployment compensation is available, making sure we are looking out for people who have lost their jobs," she said.

"这是个实证,证明我们必须确保人们都可以得到失业补偿,确保那些失去工作的人们得到照顾。"