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Extracted DNA by CTAB, in proportion to the additions of PVP and β-mercap toethanol to the extraction buffer were capable of preventing browning from taking place in the extraction, Tris-Phenol (Stabilized with 0.1M Tris, pH 8.0)/Chloroform/isoamyl alcohol were used to remove protein and impurity in the second extraction, during crude DNA used to Isopropanol DNA and deterged by DNA detergent, without using RNase remove RNA, we detected the quality and concentration of DNA respectively for normal leaves and drought stress leaves by Agarose gel electrophoresis of genomic DNA and ultraviolet spectrometry photo.

用20%聚乙二醇(polyethylene glycol，PEG6000)模拟干旱，采取CTAB提取缓冲液，按一定比例加入PVP和β-巯基乙醇，用Tris平衡酚－氯仿－异戊醇除去蛋白质及杂质，在粗提时用异丙醇沉淀DNA，并用DNA洗液（10mmol/L醋酸铵，75%乙醇）洗涤DNA，在纯化时用乙醇沉淀DNA，且没有加入RNase去除RNA的方法，分别提取2个品种正常和干旱胁迫的苹果属叶片总DNA，并对提取的DNA进行琼脂糖凝胶电泳和紫外分光光度计检测。

Supreme service: Two health specialists will take care of various parts of your body by kneeing on back, stepping on back and massaging with hands and feet with essential oil, which can improve your blood circulation, enhance cell reproduction, remove toxins from your body and make your blood vessels unblocked, thus helping you to remove fatigue, relieve tension, activate muscles, channels and collaterals, and make your whole body refreshed and comfortable.

51至尊：由两位技师对你全身各种不同部位进行护理，保健，整个过程分为跪，踩，肘，手，足点油，改善血液循环，增强细胞增生，排除体内毒素，是全身血管畅通，达到消除疲劳，缓解紧张，舒筋活络，全身爽快的目的。

Shampooing is the only way to remove ground in dirt and stains that vacuuming will not remove.

洗涤是唯一的方法除去地面赃物和吸尘器无法清除的方法。

The Rectisol process is a physical absorption method of using the excellent character of low temperature methanol to remove the light hydrocarbon, carbon dioxide, sulfureted hydrogen, carbon oxysulfide, cyanide and so on, and it can remove the sulfides from the synthesis gas by 0.1ppm.

低温甲醇洗法是利用低温下(-50℃～-70℃)甲醇的优良特性脱除原料气中的轻质油、二氧化碳、硫化氢、硫氧化碳和氰化物等的物理吸收法，能将合成气中的硫化物脱至0.1ppm。

First remove the light units from the car by unbolting the 2 bolts at the top then remove the plastic strip below the light and use a ratchet with extension on to get at the bolts under the light unit .

首先消除因单位从汽车unbolting 2螺栓在顶端，然后删除塑胶带以下的轻型和使用棘轮与扩展，以获得在螺栓下，根据单位。

2 Should ensure that the cooling pond, cleaning, there must be no debris, dirt, every six months to carry out a sea of washing machines, water pipes, to remove scale, it would be specific about 25 kg of hydrochloric acid into the plastic bucket, then on the pump inlet and outlet pipe in plastic buckets together Add to the closure of machine power, a separate 15-minute run to open pumps, hydrochloric acid in the sea in circulation, remove furring.

3.2 应确保冷却水池内清洁，不得有杂物、污垢，每六个月要对机器水路进行一次清洗，除去水管内水垢，具体做法是将25kg左右的盐酸倒入塑料桶中，然后将水泵上的进水管和出水管一并放入塑料桶中，关闭机器电源，单独开启水泵运行15分钟，使盐酸在水路中循环流动，可以除去水垢。

In order to safely remove the right side, you'll have to shift the front derailleur onto the smallest chainring, and then carefully remove the chain and rest it on the bottom bracket, so that it doesn't interfere with the crank arm removal.

为了能够安全删除右边，您就已经把前拨变速器上最小的chainring ，然后小心移除链和休息，这对底部的支架，以便它不干预与曲柄臂罢免权。

In the old days, if you found overspray or any type of unwanted substance on the hood, roof or deck lid of your car that washing didn't remove, you could simply go to the garage and grab any old compound, and together with a rag, you could quickly remove the offending contaminants. After that you could simply apply just about any companies wax and presto-chango, the problem was gone.

在早期，如果你在你爱车的引擎盖、车顶、底盘上发现清洗不掉的喷涂物或任何令人讨厌的物质，你唯一能做的就是拿著你的旧式研磨剂去车库，然后使用抹布搭配研磨剂一起来快速的移除这些令人烦扰的脏污，之后你可以简单上些任何公司所生产的蜡品，转眼间就可以解决这样的问题

The technology of separation and purification of the interesting protein was studied, centrifuge the sample and get the clear supernatant, remove protein by 50％ ethanol, concentrate by drying with freezing way, remove salt and pigment by dialysing, separation with G-50 and CM-cellulose52 column, concentrate by PEG6000, The purified sample was a single band after separation by SDS-PAGE.

研究了目的阿片肽的分离纯化工艺。发酵混合物经过离心取上清，乙醇沉淀去蛋白，冷冻干燥浓缩，透析除盐脱色，聚乙二醇6000反渗透浓缩，G-50柱分离，CM-cellulose52阳离子交换层析，聚乙二醇6000浓缩等步骤后得到了较纯的目的产物，目的产物在SDS-PAGE上呈现单一条带。

To remove a name from the right hand list box,click on it and then on Remove.

如果要把右侧列表框里的某一个名字删除，你可以通过选定名字，然后单击删除按钮来完成。

Now he worked in ajoint venture.

现在他在一家合资企业工作。

More troops in Afghanistan won't change any of the foregoing.

更多的在阿富汗的军队也不会改变之前的状况。