查询词典 purified

We optimized reaction system for labelling-antibody in which the optimal amount of the purified anti-Stx2B IgG conjugated with colloidal gold beads was 60μg/mL, the purified antibody for E.coli O157 was 57μg/mL, the optimal pH was 8.2, the size of colloidal gold partical was 20nm, the optimization of stabilizing agent was BSA, the optimization buffer was boracic acid buffer with pH 8.2, the optimization of preserving fluid and eluant was boracic acid buffer with pH 8.2 including 5mM NaCl-1%BSA, confining liquid for NC membrane was 0.01% PBST with 3%BSA, the amount of polyclonal antibody against E.coli O157 and Stx2 conjugated with colloidal gold beads for conjugate pad was 3μg respectively, the amount of anti-Stx monoclonal antibody for test line was 0.1μg and 1μg for E.coli O157, the amount of goat anti rabbit IgG for control line of both GICA were 1μg.

测定了胶体金颗粒的最优化反应体系：胶体金颗粒的大小为20nm；抗体与胶体金溶液结合的最佳pH约为8.2；最佳蛋白结合量分别为抗大肠杆菌O157 IgG为57μg/mL，抗重组Stx2B IgG为60μg/mL；最佳稳定剂为BSA；最佳缓冲液为pH8.2硼酸溶液；最佳金标保存液和洗涤液为5mM NaCl-1%BSA的pH 8.2的硼酸缓冲液；NC膜的封闭液为3%BSA的0.01mol/L PBST；Stx2试纸条和大肠杆菌O157试纸条的质控线上的羊抗兔IgG的多克隆抗体最佳点样量均为1μg，其检测线的抗Stx2的单克隆抗体的点样量为0.1μg、抗大肠杆菌O157的单克隆抗体的点样量为1μg，结合垫上的金标抗大肠杆菌O157和重组Stx2B多克隆抗体点样量均为3μg。

With the purified α-NT-linked affinity chromatography, nicotinic acetylcholine receptor is purified from the electric organ of ray .

以α-NT制作亲和胶柱，纯化了黑斑双鳍电鳐电器官中的烟碱型乙酰胆碱受体。

The factors included the type of extractant, the amount and the extraction times, the type of purified column, the type and dosage of eluviatant about the pre-treatment of the samples. The extraction was 3 minutes (13500rpm) of high speed homogenize extraction. 50mL acetonitrile as an extractant was acted on one time extraction. The florin silicon column was taken as the purified column. The extraction was eluted by 30ml 3:2 acetone - hexane and concentrated by nitrogen blowing under 50 °C water bath.The chromatographic separated conditions were studied applied the Agilent 6890 gas chromatographic apparatus and DB-17 capillary column(30m×0.25mm×0.25μm).

研究了提取剂种类、用量与提取次数，净化柱种类，淋洗剂种类与用量，提取方式与提取时间，柱温和柱流量等因素对油菜籽中有机磷农药残留提取、分离净化和色谱分离的影响，建立了采用高速匀浆提取方式提取3min13500rpm.min~(-1，以50mL乙腈为提取剂一次提取，以弗罗里硅柱为样品液净化柱，以30mL 3：2丙酮-正己烷为淋洗剂，在50℃水浴条件下用氮吹浓缩，气相色谱检测的油菜籽有机磷农药多种残留检测技术。

Purified GO could oxidize both glycolate and glyoxylate, and both activities highly depended on FMN. The activity of glyoxylate oxidation was weaker than that of glycolate. During purification or storage of purified GO, the ratio of glycolate to glyoxylate oxidation was progressively enhanced.

纯化的菜心叶片乙醇酸氧化酶对乙醇酸和乙醛酸均有氧化活性，且两者都强烈依赖于FMN，但对乙醛酸的氧化能力相对较弱；在酶的纯化及纯化酶的贮存过程中其两催化活性的比值均呈逐渐升高的趋势。

The recombinant baculovrius expressing the human antibody genes was isolated by co-transfection recombinant plasmid DNA with liner baculovirus DNA and purified by plaque assay, the intact recombinant human antibodies IgG-IV-2 and IgG-IV-6 was expressed in insert cells sf-9 after recombinant baculovrius infection and purified by protein G affinity chromatography.

通过空斑纯化，获得表达人抗体基因的重组杆状病毒，感染昆虫细胞进行抗体的全基因表达，表达产物经G-蛋白亲和层析纯化。

A technique of purified human albumin from the FIV of cold ethanol method was studied. The precipitate of FIV was dissolved and filtrated, then purified by DEAE Sephadex FF and CM Sephadex FF Chromatography. The final product was in ratter high purity.

以低温乙醇法分离血浆蛋白过程中的 FIV组份为原料，经 DEAE和 CM两步离子交换层析法，可制备出高纯度的人血白蛋白，该制品不含多聚体。

Citri had been selected by ribosome display in previous research. In order to express the scFvs with bio-activity efficiently, the genes of the selected scFvs were transformed into Escherichia coli HB2151 for expressing resoluble antibodies. The expression levels of scFvs were detected by dot blotting and Western blotting, and the expressed scFvs were purified by affinity chromatography, and then the activity of the purified scFvs was tested by enzyme-linked immunosorbent assay .

Citri， Xac高亲和力单链抗体(Single chain variable fragment， scFv)GX13、GX44和GX95，为高效表达具有生物活性的单链抗体，本研究将前期筛选的高亲和力单链抗体基因转入大肠杆菌HB2151中进行可溶性表达，点印迹和Western印迹检测可溶性单链抗体的表达水平，亲和层析纯化表达的单链抗体，酶联免疫吸附法检测纯化单链抗体的抗原结合活性。

According to breeding objective and the study on characteristics of wild southernwood resources, the plant types of southernwood were directionally screened by derived system method and group mixed method, the selected population were purified and their characters were identified, the variety comparative tests in muti-sites for many years were conducted for the purified types that passed identification.

方法］根据育种目标和对青蒿野生资源特征特性的研究，采用派生系统法和集团混合法对青蒿植株类型进行定向筛选，对中选群体进行纯化和性状鉴定，对通过鉴定的纯化类型进行多年多点品种比较试验。

Methods Fresh litchi peel was air-dried, extracted with 70% ethanol solvent in ultrasonic wave. The extract was then purified with D101 macroporous resin adsorption chromatography, C18 solid phase extraction and high performance liquid chromatography. And the component purified was identified by mass spectrography.

新鲜荔枝果皮阴干，用70％乙醇溶液以超声波浸提法提取，提取液经过D101大孔树脂吸附层析、C18小柱固相萃取、高效液相色谱法分离、纯化后，采用质谱法鉴定结构。

It was further purified by ultracentrifuge and repeated precipitation and purification with ethanol to obtain the required polysaccharide components, in which MEP\|SP of molecular of 10 000～100 000 was purified and separated by...

对其中分子量为 10 0 0 0～ 10 0 0 0 0的多糖，采用DEAE 纤维素离子交换柱，以不同浓度NaCl为洗脱液，进行纯化分离，再经SepharoseCL 6B纯化得到 2种水溶性多糖MEP SP2和MEP SP3。

I want to be a big fish in a small pond.

我要做一条在小池塘里的大鱼。

While the software functioned well, we did not feel the same about their customer support!

虽然该软件运行良好，我们没有同样的感觉他们的客户支持！