查询词典 purified

It was further purified by ultracentrifuge and repeated precipitation and purification with ethanol to obtain the required polysaccharide components, in which MEP\|SP of molecular of 10 000～100 000 was purified and separated by...

对其中分子量为 10 0 0 0～ 10 0 0 0 0的多糖，采用DEAE 纤维素离子交换柱，以不同浓度NaCl为洗脱液，进行纯化分离，再经SepharoseCL 6B纯化得到 2种水溶性多糖MEP SP2和MEP SP3。

Results Two months after transplantation, there are significant differences in secreting function of microencapsulated parathyroid tissue between purified and unpurified groups(P＜0.05),(38.8±13.7) pg/ml and (1.2±0.7) pg/ml respectively. The retrieve rates of purified and unpurified alginate empty microcapsules are （72±18）%and 24±13）%,（65±21）%and 0 respectively after 1 and 2 months of transplantation.

结果移植后2个月纯化组及未纯化组人甲状旁腺组织分泌功能有显著差异（P.05），分别为(38.8±13.7) pg/ml和(1.2±0.7) pg/ml，纯化及未纯化海藻酸钠制备空囊移植组，植入1个月时微囊冲洗回收率分别为（72±18）%和（24±13）%，植入2个月时为（65±21）%和0。

It is shown that polystyrene has been grafted on the surface of nmSiO2 particles and the grafting ratio is about 40%. The surface of nmSiO2 could be treated effectively by KH570in absolute ethanol at 25℃, 48h. The nmSiO2-g-PSt unpurified by toluene appear microsphere in which nmSiO2 particles are contained, whereas the purified one appear internet formed by many pieces. The grafting ratio of PSt on nmSiO2 surface could be influenced obviously by dispersion agents content and agitation speed, whereas the morphology of nmSiO2-g-PSt purified is rarely changed.

结果表明：常温下在无水乙醇介质中处理48h以上可获得良好的硅烷处理效果；PSt已接枝包覆于nmSiO2表面，接枝率约40%；经甲苯洗涤前nmSiO2-g-PSt呈多粒子包覆微球状，洗涤后呈单粒子包覆网状结构；分散剂用量、搅拌速度改变对接枝率有影响，但对包覆形态影响不大。

Methods The skeletal muscle stem cells were enzymatically isolated with collagen and dispase, and then purified by different adhesion time from 6 female SD rats. The purified cells were transinfected with adenovirus carrying lac-Z gene, and injected into the bladder neck of 6 autologous and 4 allogeneic rats. Tissues of blader neck and ureter were obtained in sacrified rats after 5 and 15 days, histological examination and X-gal staining were performed.

采用混合酶消化及反复差速贴壁法分离、纯化培养SD大鼠骨骼肌干细胞，然后用携带lac-Z基因的腺病毒载体转染肌干细胞，待转染成功后将携带lac-Z基因的骨骼肌干细胞注射于自体和同种异体的膀胱颈及后尿道周围，分别于注射后5、15d，处死大鼠取出膀胱颈及后尿道，行组织学检查及X-gal染色。

The proteins were purified and used to prepare the rabbit antiserum against caprine IFN-γor IL-2,the results showed that the antibody could react with the purified proteins in Western blotting.

分别用ProBond~蛋白纯化试剂盒纯化，用所获得的重组蛋白纯化产物经三次背部皮下多点注射新西兰白兔，制备了兔抗山羊IFN-γ和兔抗山羊IL-2多克隆血清。

The result of protein concentration analysis, activity detection and sodium dodecyl sulphate - polyacrylamide gel electrophoresis demonstnated that protein content of IgG purified by ammonium sulfate is higher than that of caprylic acid - ammonium sulfate, but the purity and activity of IgG purified by caprylic acid - ammonium sulfate is far higher than that of ammonium sulfate.

本研究中对辛酸-硫酸铵法和硫酸铵法提取鸭血清中IgG进行了比较，经蛋白含量测定和SDS-PAGE电泳分析表明，虽然硫酸铵法提取蛋白含量要比辛酸-硫酸铵法高，但辛酸-硫酸铵法提取IgG的纯度和活性都明显高于硫酸铵法。

Two inducible and extracellular chitosanases secreted by Penicillium sp. D-l were purified by ammonium sulfate precipitation as well as gel filtration. The purified enzymes of ChoA and ChoB had molecular masses of 93 and 21 kDa by SDS-PAGE. Both ChoA and ChoB have an acidic pH optimum, which were 4.0. The enzymes were stable over the pH range 3 to 5 for 1 h.

采用(NH_4)_2SO_4盐析法和凝胶过滤层析对Penicillium sp.D-1的壳聚糖酶进行分离提纯，得到两种壳聚糖酶，酶A经变性聚丙烯酰胺凝胶电泳分析，结果显示该酶的分子量约为93 kD；酶B经非变性聚丙烯酰胺凝胶电泳和SDS-PAGE分析，得出分子量约为21 kD。

The free radical scavenging properties of six Aloe species are to be investigated in some modified chemical system. The antioxidant was purified by silica column and thin layer chromatography and analyzed by HPLC. A fraction was purified from A. brevifolia Mill showed the most effective scavenging activities on 〓 and ·OH (66. 01% and 38. 98% respectively). With the analysis of IR spectrum, UV spectrum and mass spectrum, the structure of FA was identifed as 8-C-D-glucopyranosyl-2- [2-hydroxy] propyl-5-methyl-7-hydroxychromone. Its scavenging activity on free radicals is higher than that of aloe-emodin. Its antioxidant activity may be due to the phenoxyl on glucosyl chromone which can be react with free radicals and form relatively stable free radicals.

本研究利用分别产生超氧阴离子自由基和羟自由基的化学模型体系研究比较了不同品种芦荟提取物的清除活性氧自由基的抗氧化作用，进一步利用硅胶柱结合薄层层析法和反相高压液相色谱法，从短叶芦荟叶子中首次分离了具有较强自由基清除能力（超氧阴离子自由基和羟自由基清除率分别为66.01％和38.98％）的成分FA，利用红外光谱、紫外光谱和质谱等仪器分析结果推断出该活性成分的分子结构为8-C-吡喃葡萄糖-2-[2-羟基]-丙基-5-甲基-7-羟基对氧萘酮，它的自由基清除能力高于芦荟大黄素，是一种天然抗氧化剂。

The TC gene was screened from cDNA library of adult Clonorchis sinensis, cloned, expressed and purified. The purified protein of TC gene will be of importance for further research on the biological function of the gene.

通过生物信息学方法由华枝睾吸虫成虫cDNA文库中筛选出TC基因，并成功予以克隆，表达及纯化。

Methods: Adenosine deaminase was purified by immtino-affinity chromatography, the enzyme activity was assayed colorimetrically by the measurement of ammonia produced over a fixed timeResults: The purified ADA was shown to be a homogeneity by polyacrylamide gel electrophoresis.

用免疫亲和层析法分离纯化人肾ADA，测氨法测定 ADA活性，经SDS-PAGE测定其分子量。

He is the most remarkable man that I have ever known.

他是我知道的人中最值得注意的人。

Increased appearance of lymphocele was noticed in patients with diabetic nephropathy, congenital malformations of the urinary tract, and inflammatory diseases, including glomerulopathy and extraglomerular ones, after high-voltage radiotherapy and after removal of the renal graft.

经过高压放射疗法和切除移植肾后，在糖尿病肾病患者、泌尿系统先天性畸形患者和炎症患者身上发现淋巴囊肿有所增加，包括肾小球病和球外疾病等。