查询词典 purified

In order to test the antibodies to E6 and E7 induced by the recombinant vaccina virus, the fusion proteins of GST and E6 or E7 were expressed in E. coli using pGEX-2T vector. The expressed proteins were confirmed by Western-blot and purified by glutathione sepharose CL-4B affinity chromatography. The purified proteins were used to detect the anti-E6 and-E7 by ELISA.

为便于检测重组痘苗病毒所诱发的E6和E7抗体，本文利用原核表达载体pGEX-2T，在大肠杆菌中分别高效表达了GST与E6或E7的融合蛋白，并利用纯化的融合蛋白成功地建立了E6和E7抗体的ELISA检测方法。

The products were identified by IR,NMR,MS and elemental analysis. In our method,K.OH was used in place of NaOH to synthesize benzyl ester of 3,5-dimethyl-4-hydroxybenzoic acid,2',6'-dimethyl-4'-(n-succinimidyloxycarbonyl) phenyl-acridinium-9-carboxylate was purified on a silica gel column with chloroform/ethylacetate(4:l,v/v) as eluent and further purified by triturating with hexane/acetone(2:l,v/v).The luminescence produced by DMAE-NHS is a flash light with maximumemission at 0.4s and decay half-time of 0.9s. The luminescence intensity is 6.11x10 cps/mol,which is affected by the composition of trigger and surfactant.

DMAE·NHS的合成是本论文的关键和难点，我们对文献方法进行改进：文献方法用氢氧化钠与3，5-二甲基-4-羟基苯甲酸反应制得3，5-甲基-4-羟基苯甲酸钠，再用3，5-二甲基-4-羟基苯甲酸钠与苄氯作用制备3，5-二甲基-4-羟基苯甲酸苄酯，我们用氢氧化钾代替氢氧化钠，使合成取得成功；在合成2'，6'-二甲基-4'-苯基-吖啶-9-甲酸酯时，文献方法对粗产品进行两次硅胶柱层析纯化，第一次柱层析用氯仿/乙酸乙酯(4：1，Ⅴ：Ⅴ)作溶剂和淋洗剂，第二次柱层析用己烷/丙酮(2：1，Ⅴ：Ⅴ)作溶剂和淋洗剂，按照文献方法得到的不是所需要的化合物，我们只进行第一次柱层析纯化，然后用己烷/丙酮(2：1，Ⅴ：Ⅴ)进行研磨，过滤，洗涤，除去溶于己烷/丙酮(2：1，Ⅴ：Ⅴ)的部分，得到所需要的产品。

With Ammonia as refrigerant and water as absorbent, they are boiled by the heater in the generator, as a result, the Ammonia-water solution is evaporated. The Ammonia vapor is purified by the rectifier and is condensed into liquid Ammonia in condenser. The liquid Ammonia and the pure Helium purified in the absorber are evaporated together in the evaporator and to absorb heat thus refrigerate at the same time.

采用氨为制冷剂，水为吸收剂，通过对制冷机芯的发生器加热，使氨水溶液中的氨蒸发，氨蒸汽经精馏器提纯后进入冷凝器成液氨，液氨在蒸发器中与经吸收器提纯的纯氦共同蒸发，同时从外部吸收热量，达到制冷的目的。

The PCR products were examined by agarose gel electrophoresis. The target gene fragments were purified by gel extraction kit and ligated to cloning vector pMD18-T. The recombinant vectors were transformed into host strain E. coli K802 by lithium chloride method, screened and identified with PCR and restrictive enzymatic digestion. Their sequences were confirmed by DNA sequencing.(2) sTWEAK1 gene was subcloned into expression vector pProEx HTb and transformed into E. coli BL21. sTWEAK2 gene was subcloned into expression vector pMAL-C2x and transformed into E. coli TB1. The recombinant vectors were screened and identified with PCR and restrictive enzymatic digestion. The recombinant fusion proteins were induced to express with IPTG, detected by coomassie brilliant blue-stained SDS-polyacrylamide gel electrophoresis , and confirmed by Western blot analysis.(3) The sTWEAK1 fusion protein was purified with Ni-NTA Spin Kit.(4) The biological activity was assayed on transformed and tumor cells by microplate photometer after crystal violet or sulfur rodamine B staining.(5) The contents of IL-8 in the supernatant of 1990 cell cultures were determined by ELISA.(6) The morphological changes of the sensitive cells were observed by light and transmission electron microscopies.(7) The cell cycle and apoptotic rate were assayed by flow cytometry in 1990 and M85 cells.(8) The effect of fusion proteins on induction of NF-κB in 1990 and LOVO cells was detected with Dual-Luciferase Reporter Assay system.(9) The TWEAK gene was subcloned into Adeno-X Viral DNA with pShuttle vector and transfected into HEK293 cells by lipofectamine method.

(1)本研究用RT-PCR方法，从人组织细胞总RNA中扩增可溶性TWEAK胞外区（sTWEAK1和sTWEAK2）的cDNA序列及全长编码序列，用琼脂糖凝胶电泳分析PCR产物，胶回收目的基因片段，连接到pMD18-T克隆载体中，转化大肠杆菌K802，PCR和酶切筛选阳性克隆，全自动DNA测序验证序列；(2)sTWEAK1和sTWEAK2分别亚克隆到pProEx HTb和pMAL-C2x表达载体中，分别转化大肠杆菌BL21和TB1，PCR筛选和酶切鉴定，阳性克隆用IPTG诱导表达，表达产物用SDS-PAGE分析和Western blot验证融合蛋白；(3)用NTA-Ni Spin试剂盒初步分离纯化sTWEAK1融合蛋白；(4)用体外培养的肿瘤细胞和正常对表达产物进行活性检测，贴壁细胞用结晶紫染色法，悬浮细胞用磺酰罗丹明B染色法，酶标仪检测OD值；(5)敏感细胞用ELISA法检测细胞培养上清中IL-8的含量；(6)用光镜和电镜观察敏感细胞死亡和细胞凋亡情况；(7)用流式细胞仪分析表达产物对敏感细胞凋亡率和细胞周期的影响；(8)用双荧光素酶报告基因检测法，测定表达产物对敏感细胞NF-κB的影响；(9)用pShuttle穿梭质粒将TWEAK重组到腺病毒载体上，用脂质体转染法转染HEK293细胞，PCR鉴定重组质粒。

The company existing staff 300 people, among, the senior technical personnel several people and have specialized technical the staff 200 peopleThe company leads the product is "ANAI" the sign conveyor belt,Including the ordinary use fabric core conveyor belt (nylon cloth wick, polyester cloth wick, cotton-dacron mix core, purified cotton cloth wick and so on), the special use fabric core conveyor belt (thermostable, is heat-resisting, is flame-resistant, bears acid and alkali generally, oil resistant, food and so on, cloth wick has nylon cloth wick, polyester cloth wick, cotton-dacron mix core, purified cotton cloth wick and so on).

公司现有员工300人，年生产各类输送带1000万平方米。公司主导产品是&安耐&牌输送带，包括普通用途织物芯输送带尼龙布芯、聚脂布芯、涤棉布芯、纯棉布芯等），特殊用途织物芯输送带（耐高温、耐热一般阻燃、耐酸碱、耐油等，布芯有尼龙布芯、聚脂布芯、涤棉布芯、纯棉布芯等），各种异型输送带。

METHODS: The rhBMP-2,expressed in Escherichia coli, was washed by Triton X- 100 and further purified by DEAE chromatography.The inclusion bodies were resolved in 8 mol/L urea, and were refolded and dimerized in the redox systems (reduced and oxidized glutathione). Finally, a one - step purification procedure based on the heparin affinity chromatography was implemented. The biological activity of purified rhBMP - 2 were tested by induction of the alkaline phosphatase activity in C2C12 cells.

在大肠杆菌中通过温度诱导表达重组人骨形成蛋白-2经过Triton X-100清洗之后，又通过DEAE离子交换层析纯化包涵体，包涵体在8 mol/L尿素变性溶解，在氧化-还原(还原型和氧化型谷胱甘肽)复性系统中，通过简单的稀释复性，通过肝素亲和层析一步纯化法纯化重组人骨形成蛋白-2，最后通过诱导C2C12细胞产生碱性磷酸酶检测重组人骨形成蛋白-2活性。

The purification method is that the oxalic acid difluoro lithium borate needing to be purified is dissolved in solvent with high solubility first, then the solution is mixed with nucleation agent, the mixed solution is performed through solid-liquid separation, crystallized solid matters are put in a vacuum drying oven for drying, so that purified LiODFB products are obtained.

一种草酸二氟硼酸锂的提纯方法，该方法是先将需提纯的草酸二氟硼酸锂溶解在溶解性高的溶剂中，然后将该溶液与析晶剂进行混合，通过固液分离，将析晶出的固体物质置于真空干燥箱中干燥，即得纯化的LiODFB产品。

The animal model is established successfully. The purified main allergen could greatly improve the test of sIgE titer, which suggest that the purified allergen can be used in hypersensitivity diagnosis and therapy of sea shrimp.

结果表明，海虾过敏症豚鼠模型建立成功，并且纯化的海虾过敏原组分应用于ELISA检测能提高sIgE的检测水平，对海虾过敏症的体外诊断和治疗有一定的意义。

Western blots were performed to investigate the cross-reactivity of various labeled antibodies with serum proteins from other fish species. Results Immunoglobulins of five fish species were purified, including bighead carp, carp, argus snakehead fish, ricefield eel, sea bass. The titers of five rabbit anti-sera obtained up to 1:32 examined by agarose double immunodiffusion. Five kinds of purified rabbit anti-fish immunoglobulins antibodies were labeled with HRP and their activities were up to 1:10000 examined by EUSA. Four kinds of rabbit anti-fish immunoglobulins antibodies labeled with HRP could react with serum proteins from other fish species.

结果 纯化了鳙、鲤、乌鳢、黄鳝、鲈五种鱼血清免疫球蛋白，免疫双扩散法测定兔抗这五种鱼免疫球蛋白的抗血清效价均达到1：32，并对纯化的兔抗鳙、鲤、乌鳢、黄鳝、鲈五种鱼类免疫球蛋白的抗体进行了HRP标记，ELISA测定标记抗体的效价达到1：10000左右，Western blots显示标记抗体与部分其他鱼类免疫球蛋白之间存在不同程度的交叉反应。

Four further single mutants ofN546(N546G,N546D,N546K,N546Δ), three double mutants and a triple mutant were constructed,and toxins were purified by isoelectric precipitation and an AKTA purifier 100 system.When bioassayed against H.armigera using purified toxins,N546ALC_(501.67μg/mLshowed 1.78 times increased toxicity than wide-type Cry1AcLC_(50 2.98μg/mL,and mutant N546D,N546K and N546Δshowed a great loss in toxicity.

对N546进一步突变，获得了突变子N546G、N546D、N546K和删除突变N546Δ及3个双突变和1个三突变，利用等电点沉淀法和AKTApurifier 100系统纯化了毒素蛋白，生测发现N546ALC_(50为1.67μg/mL对棉铃虫毒力比野生型Cry1AcLC_(50为2.98μg/mL提高了1.78倍，而N546D、N546K和N546Δ的毒力则降低了80～90%。

He is the most remarkable man that I have ever known.

他是我知道的人中最值得注意的人。

Increased appearance of lymphocele was noticed in patients with diabetic nephropathy, congenital malformations of the urinary tract, and inflammatory diseases, including glomerulopathy and extraglomerular ones, after high-voltage radiotherapy and after removal of the renal graft.

经过高压放射疗法和切除移植肾后，在糖尿病肾病患者、泌尿系统先天性畸形患者和炎症患者身上发现淋巴囊肿有所增加，包括肾小球病和球外疾病等。