查询词典 processing sequence

The genetic DNA was extracted from the blood of 6 A. forsteri which sex was unknown. The PCR amplified fragments with primers P2/P8 were cloned in T-Vector. Taking homologous sequence of Circaetus gallicus as reference, the CHD gene sequence was compared to the homologues in GenBank to identify its sex.

方法］采用苯酚：氯仿抽提法提取6只未知性别的帝企鹅血液中的基因组DNA，运用P2/P8引物扩增CHD基因片段，将PCR产物克隆到T-Vector，利用NCBI的Blast程序，以短趾鹰同源序列为比对参照，将帝企鹅CHD基因片段序列与GenBank中的基因片段序列进行同源性比较分析，鉴定帝企鹅的性别。

Materials and Methods: Comparetive study between 2D - SE sequence and 3D-CISS sequence was made for lesions detection and diagnostic accuracy in 8 cases of intraventricle and 12 cases of cisternal diseases.

材料和方法：对照分析8例脑室和12例脑池占位病变的常规SE序列和3D—CISS的检出率和诊断正确率。

An otherwise healthy 12-month-old girl presented for evaluation of reduced abduction of the left eye detected at 6 months of age. The remainder of the examination was unremarkable. A special MRI sequence-fast imaging employing steady-state acquisition-visualized the right but not the left sixth nerve cisternal segment. This is the first reported use of the MRI FIESTA sequence to diagnose aplasia of the sixth cranial nerve.

应用快速平衡稳态取像核磁技术检测第六颅神经发育不全：本研究首次报道利用 MRI FIESTA 诊断第六颅神经发育不全，一例12个月的婴儿其他各项指标正常，在6个月时发现左眼诱导注视减少，其他的检查未见明显异常，利用 MRI FIESTA 可检测到右侧第六颅神经，但左侧部分检测不到。

A first embodiment comprises a first common primer sequence, a first template homology region, a cleavable site, a second common primer sequence and a second template homology region.

第一个实施方案包含第一通用引物序列、第一模板同源性区、可切割位点、第二通用引物序列和第二模板同源性区。

The combinant vector was used as a template for PCR which was cloned into vector pMD-19T,then subcloned into expression vector pET-26b with pelB signal sequence and His-Taq sequence.After transforming E.coli BL21(DE3) and four hours induction by IPTG,HMGB1 expression confirmed by SDS-PAGE and the purification was performed by Ni2 -chelate affinity chromatograph.

脂多糖刺激后的RAW264.7细胞，提取总RNA，经RT-PCR扩增出含HMGB1的目的片段，克隆于pMD-19T载体，再亚克隆至含有pelB引导肽及His-标签肽的高效表达载体pET-26b，转化大肠杆菌BL21（DE3），经IPTG诱导后行SDS-PAGE鉴定目标蛋白表达，用镍鳌合琼脂糖凝胶亲和层析法分离纯化含His-标签肽的目的蛋白。

Based on the analysis of the linear congruential generator and TigerSHARC20XS of ANALOGDEVICES,this paper presents a method for generating the pseudo random sequence in optional cycle by addressing the sequence of descending length.

文中基于线性同余算法，结合Analo Gdevices公司DSP芯片TigerSHARC20XS的运算结构，设计出一种利用寻址递减长度序列，从而产生具有遍历性的任意长度伪随机序列的方法。

Three uniform random sequences are built by the linear congruential method, of which two are used to form integer num, ber and decimal fraction parts of the new random sequence respectively and the third to shuffle the new sequence.

采用线性同余法生成3个均匀分布随机序列，其中两个分别组成新的随机序列的整数和小数部分，第3个用来对新序列进行混洗。

Strains YL001 and YL002 formed a monophyletic clade with strains of X. nematophilus with sequence homology outweighing 99% and the sequence homology to genus Photorhabdus outweighing 94%. The lag, logarithmic, stationary and contabescence phase of the two bacteria were 0-6, 6-18, 18-66 and 66 h respectively; pH of strains YL001 and YL002 reduced to 5.70 and 5.56 at 12 h, respectively, and then rose gradually to 7.74 and 8.07 respectively when culture finished. Glucose content reduced quickly during 0-18 h, and then kept stable. Amido nitrogen content reached the lowest at 12 h and then rose slowly. YL001 exhibited the highest inhibitory effects at 54 h and YL002 exhibited highest inhibitory effects at 42 and 66 h respectively on B. cirerea and B. subtilis. Strains YL001 and YL002 belonged to X.

YL001和YL002菌株与嗜线虫致病杆菌种内菌株形成一个类群，序列同源性大于99%，与发光杆菌属内菌株的序列同源性大于94%。2菌株的延缓期、对数生长期、稳定期和衰亡期分别为0~6，6~18，18~66和66 h；培养12 h后，YL001和YL002菌株发酵液的pH值分别降低至5.70和5.56，此后逐渐上升，至发酵结束时其pH值分别为7.74和8.07；培养0~18 h时2菌株发酵液中还原糖含量迅速降低，此后保持稳定；12 h时氨基氮含量达到最低，此后开始缓慢上升；YL001菌株培养54 h后及YL002菌株培养42和66 h后，其对番茄灰霉病菌和枯草芽孢杆菌的抑制作用最强。

Japonicus used in this experimentation is matemal inheritance.5 Sequence analysis of mitochondrial 16S rRNA and COI gene and molecular phylogeny of four species of sea cucumber500 bp 16S rRNA and 540 bp COI fragment were obtained by PCR method from mitochondrial DNA of four species of sea cucumber, Apostichopus japonicus, Holothuria fuscogilva, Cucumaria frondosa and Mensamaria intercedens. Variable sites, nucleotide diversity, average number of nucleotide differences and average number of nucleotide substitutions per site were calculated to analyze sequence difference, and phylogenetic trees were constructed by the NJ and ME method.

结果显示在来自这5组家系的100个后代中仅检测到母本的单倍型，刺参的线粒体遗传模式表现为典型的母系遗传。5 4种海参16S rRNA和COI基因片段序列比较及系统学研究采用PCR技术对山东荣成、长岛、俄罗斯和日本的刺参，黄乳海参，北大西洋瓜参，二色桌片参的16S rRNA和COI基因片段进行了扩增和测序，分别得到了长度约为500 bp和540 bp的片段。

After the multiple sequence alignments, the sequence variation analyses were conducted and the molecular phylogenetic trees of Cypraeidae were reconstructed with NJ, MP and bayesian methods, using Prosimnia semperi of Cypraeacea as an outgroup.

其次，用自测的宝贝科11种软体动物的mtDNA COI基因序列，结合得自GenBank上的18种宝贝科软体动物的同源序列，用NJ法、ML法以及贝叶斯法构建了分子系统树。

Pachomius manual labour was organized as an essential part of the monastic life; and since it is a principle of the monks as distinguished from the mendicants, that the body shall be self-supporting, external work of one sort or another has been an inevitable part of the life ever since.

根据圣pachomius体力劳动组织作为一个重要组成部分寺院生活；以来，这是一个原则的和尚，作为有别于乞丐，该机构应自支撑，对外工作的这种或那种已一个必然的一部分，生活至今。

In order to study the effects of parametrical rolling on the ship movement and tilts, in allusion to ship's navigating in regular following waves, the equation of ship rolling motion is transformed into normal Mathieu equation by proper variable replacement when strong parametrical excitation in restoring moment varied according to sine or cosine wave.

为研究参数横摇对船舶运动和倾覆的影响，通过对船在规则波上随浪航行时，恢复力矩中出现的1个以正余弦规律变化的强参数激励项进行适当的变量置换，将船舶的横摇运动方程转换成标准形式的Mathieu方程。