查询词典 processing sequence

Two gene fragments of DEN-1(GenBank accession number DQ886390)and DEN-2(GenBank accession number DQ886391),differentially expressed between the ocular skin tissues of normal and albino turbot,were isolated by mRNA differential display and sequenced.It was confirmed by RT-PCR method that the expression levels of both DEN-1 and DEN-2 were down-regulated in the ocular skin tissue of albino turbot.The sequence homology search of DEN-1 revealed high sequence homologies with the UGGT genes of zebrafish and cattle,and the Ugcgl1 gene of Norway rat.High sequence homologies of DEN-2 were observed with the eya4 gene(the eye absent homolog 4)from red jungle fowl,zebrafish,human,Norway rat,mouse and dog.

应用mRNA差异显示技术，对比研究正常与白化大菱鲆有眼侧皮肤组织，克隆差异表达基因，经测序，RT-PCR 验证，差异表达基因片段DEN-1(GenBank登录号：DQ886390)与DEN-2(GenBank登录号：DQ886391)均在白化大菱鲆有眼侧皮肤组织中下调表达；通过BLAST检索发现，DEN-1与GenBank中的斑马鱼和牛的类似尿苷二磷酸-葡萄糖：糖蛋白葡糖基转移酶基因、与挪威鼠类似尿苷二磷酸-葡萄糖：神经酰氨葡糖基转移酶1(Ugcgl1)基因有较高同源性；DEN-2 与原鸡、斑马鱼、人、挪威鼠、家鼠和狗的眼缺乏同源框4(eya4)基因的同源性均较高。

If one sequence is shorter than the other and all its elements match the corresponding elements in the longer sequence, then the shorter sequence is lexicographically smaller.

如果一个序列比另一个短，并且它的元素与较长序列中对应元素相匹配，则较短的序列在字典序上较小。

Pairwise sequence alignment is one of common methods in analyzing sequence, which also is the base of multiple sequence alignment and data search.

双序列比对是序列分析常用的方法之一，也是多序列比对和数据库搜索的基础。

In order to compare the homology of the gene encoding VP2 between MDPV-Q and MDPV which was registered in the GenBank, also in order to find out changes of the VP2 gene and the immuno-genicity between the wild-strain MDPV-Q and the attenuated strain MDPV-26 which derived by continued passage of virulent wild-type in muscovy duck embryo, a pair of primer (LHMP7/LHMP8) was designed. The upper one LHMP7 and the lower one LHMP8 corresponded to the MDPV-specific nucleotides sequence 2885-2900 and 4618-4604 respectively, according to the MDPV nucleotide sequence databases. The length of the sequence embraced by the primers was 1734bp.

为了获取这一基因片段与国外分离株进行同源性比较，同时也为了了解MDPV强、弱毒株VP2基因之间的异同关系，及其免疫原性的变化规律，通过DNA重组技术，设计了一对引物LHMP7／LHMP8，该对引物选取位于2885～2900及4618～4604的两段序列，跨幅为1734bp，并在这两条引物中分别加入两种限制性核酸内切酶SacⅡ和KpnⅠ的酶切位点，分别对MDPV-Q和由该株病毒经人工致弱后得到的MDPV-26株进行PCR扩增，并将PCR产物克隆到pMD18-T载体上，分别得到二个重组子：pMD18-T—M-Q VP2和pMD18-T-M-26 VP2。

The amplified band from M131 was sequenced and then aligned with the sequence of avr-Pila gene. The result was that the sequence was 98% homologous to one fragment of avr-Pita sequence, but the integration site was not within this fragment.

将M131的扩增片段测序后得到长度为1057bp的序列，并且将所测序列和已知的avr-Pita一段序列比较发现达98％的同源，即该序列为无毒基因的某一段序列，但插入的位点并不在所扩增片段内。

The amplifiedband from M131 was sequenced and then aligned with the sequence of avr-Pila gene. The result was that the sequence was 98% homologous to one fragment of avr-Pita sequence, but the integration site was not within this fragment.

将M131的扩增片段测序后得到长度为1057bp的序列，并且将所测序列和已知的avr-Pita一段序列比较发现达98％的同源，即该序列为无毒基因的某一段序列，但插入的位点并不在所扩增片段内。

The PCR product was cloned into PGEM-T-Easy DNA plasmid by TA cloning, and hINS gene was analyst and compared to the published sequence ,and found that this hINS gene is same to one of the published sequence and there are four different bases with the other published sequence.

为了进一步验证扩增出的hINS基因能否在蛋白质水平上表达，用构建的乳腺特异性表达载体制作转基因小鼠，利用转基因小鼠作为个体表达系统来检测目标蛋白表达量。

By NCBI BLASTX server, tomato P56 amino-acid sequence was compared with other pectate and pectin lyases belonging to polysaccharide lyase family Ⅰ 28 proteins ,with higher identity of amino acid sequence compared to P56 amino-acid sequence, were obtained.

利用NCBI BLASTX服务器，搜索与番茄P56蛋白氨基酸序列相似且都属于多糖裂解酶家族Ⅰ的果胶裂解酶蛋白序列共28条，用DNAMAN软件分析其保守区，用CLUSTALX8.0软件进行序列比对和Bi-oEdit软件进行文件转换，进一步用PUAP4.0软件构建系统进化树。

The species observed can be categorized into 5 kinds of Fibonacci sequence groups based on the sequence of phyllotaxy and golden divergence angle. At the same time, by comparing the existing documentation, we explore the difference and relation among different taxa, in addition, the difference between environments in order to explicate the factors influencing the sequence of phyllotaxy.

藉由叶序之序列及黄金分离角，可将观察之物种，分为5种费氏序列类群，同时比对既有的文献纪录，探讨不同分类群间的差异，以及分类群间的关系，并分析环境之间的差异，藉此说明影响叶序配列的因子。

The notion of sample relative entropy , as a random measure of the deviation of a sequence of nonnegative continuous random sequence from independent random sequence with exponential distribution, is introduced.

利用样本相对熵作为一般非负连续型随机变量序列相对于服从指数分布的独立但不同分布随机变量序列偏差的一种随机性度量，运用鞅理论及分析方法，研究了一类随机变量序列加权和的用不等式表示的极限定理，即强偏差定

Pachomius manual labour was organized as an essential part of the monastic life; and since it is a principle of the monks as distinguished from the mendicants, that the body shall be self-supporting, external work of one sort or another has been an inevitable part of the life ever since.

根据圣pachomius体力劳动组织作为一个重要组成部分寺院生活；以来，这是一个原则的和尚，作为有别于乞丐，该机构应自支撑，对外工作的这种或那种已一个必然的一部分，生活至今。

In order to study the effects of parametrical rolling on the ship movement and tilts, in allusion to ship's navigating in regular following waves, the equation of ship rolling motion is transformed into normal Mathieu equation by proper variable replacement when strong parametrical excitation in restoring moment varied according to sine or cosine wave.

为研究参数横摇对船舶运动和倾覆的影响，通过对船在规则波上随浪航行时，恢复力矩中出现的1个以正余弦规律变化的强参数激励项进行适当的变量置换，将船舶的横摇运动方程转换成标准形式的Mathieu方程。