查询词典 probe

To resolve the question about direction cosine when three-COMERO produced by COORD3 works, the formula of direction cosine of every probe is demonstrated in geometrical relationship from the explanation about gauging and qualification of probe, which provides academic proof for the probe worked correctly.

摘 要：针对COORD3公司三坐标测量机使用中有关方向余弦的疑点，从探针的标定和校正入手，对每个探针的方向余弦公式进行了几何关系上的论证，为探针的正确使用提供了理论依据。

A method for detecting the presence of a target nucleic acid sequence in a sample, said method comprising subjecting said sample to an amplification reaction using a set of nucleotides, at least one of which is fluorescently labelled, contacting amplification product with a probe under conditions in which the probe will hybridise to said target sequence, said probe comprising a reactive molecule which is able to absorb fluorescence from or donate fluorescent energy to said fluorescent labelled nucleotide and monitoring fluorescence of said sample.

一种用于检测在某样品中是否存在目标核酸序列的方法，该方法包括：使用一套核苷酸对所说的样品进行扩增反应，其中至少一个核苷酸是被荧光标记的，在探针与目标序列杂交的条件下使扩增产物与探针混合，所说的探针包括一种活性分子，该活性分子能够从荧光标记核苷酸吸收荧光或向其贡献荧光能量，及检测样品的荧光。

Fig 1 Negative control Female umbilical cord sample has no Y-specific signal after in situ hybridization with the biotinylated Y-repeated sequence DNA probe PY3.4 Fig 2 Positive control Flow-sorted male umbilical cord cells hybridized to Y-specific DNA probe PY3.4,Every cell contains a Y-specific signal Fig 3 Fetal cells sorted from maternal blood Flow-sorted cells from a pregnant woman at 20 weeks of gestation hybridized to Y-specific DNA probe PY3.4,containing a Y-specific signal Fig 4 The result of polymerase chain reaction Lane 1:male umbilical cord NRBCs sorted by FITC-conjugated anti-monoclonal glycophorin A;Lane 2:sample of 2;Lane 3:male umbilical cord NRBCs sorted by FITC-conjugated anti-CD36;Lanes 4-5:samples of 1 and 3;Lane 6:50 cells of male;Lane 7:5 cells of male;Lane 8:200ng male DNA(positive+control);Lane 9:nonpregnant female DNA(negative+control);Lane 10:ΦX174 HaeⅢ Maker,271bp:amplified band of Y-specific gene SRY;383bp:amplified band of human β-globin gene

图1 阴性对照女性脐带血标本，经与生物素标记的Y-染色体重复序列DNA探针原位杂交后未见Y-染色体特异信号图2 阳性对照分选的男性脐血细胞经与Y-特异DNA探针杂交后，每一细胞都含有Y-染色体特异信号图3 母体外周血中分选的胎儿细胞从一位妊娠20周的孕妇外周血中分选出的细胞经与Y-特异DNA探针杂交细胞中含有Y-染色体特异信号图4 聚合酶链反应结果 1：GPA-FITC单抗分选男胎脐血NRBCs；2：2号标本；3：CD36-FITC单抗分选男胎脐血NRBCs；4、5：1、3号标本；6：50个男性细胞标本；7：5个男性细胞标本；8：200ng男性DNA；9：未孕女性DNA；10：ΦX174 HaeⅢ标准，271bp：为SRY基因扩增带；383bp：为人β-珠蛋白基因扩增带内参照

The undercutting analysis probe the position of undercutting line and the spherical angle of spherical gears. Teeth contact analysis probe the kinematic error and the position of contact point of spherical gears with misalignment and ideal case. Another contact pattern analysis probe the positions and size of contact pattern ofspherical gears.

齿面过切分析主要探讨球形齿轮之齿面过切位置及过切发生的球形角，接触分析则讨论球形齿轮分别在具有装配误差及理想状况时之运动误差及接触点位置，另外，齿印分析探讨球形齿轮在受力变形后其接触椭圆之位置及大小。

Methods 2.1 The experiment about changing electricic pressure values into the pulse-taking pressure values of making pressure standars of sphygmograph. Putting 1g、2g、5g、10g、20g、50g、100g、140g weights on the black1 probe and the black2 probe of NX-3 multifunctional sphygmograph ,putting 1g、2g、5g、10g、20g、50g weights on the purple probe of NX-3 multifunctional sphygmograph. Then we noted the the different pressure numerical value when putting none and different weights on the probes to prove the liner relationship between them. 2.2 Setting up wiry pulse model in achy rabbits We operated on the non-drugged state rabbits, exposure the carotid.

2.1 关于电学压力值与实际压力值换算的脉图仪压力定标实验：NX—3 多功能脉图仪黑色探头 1 和黑色探头 2 上放置 1g、2g、5g、10g、20g、50g、100g、140g砝码，NX—3 多功能脉图仪紫色探头放置 1g、2g、5g、10g、20g、50g 砝码分别记录两种脉图仪共三个探头其空载、探头上放置砝码时脉象采集仪上所显示的取脉压力值，验证砝码重与取脉压力值的线性关系。

This suggested there might have cis-acting element in 3'-UTR. In order to search the possible trans-acting factor, we found RNA-protein band in H〓 cells and no band in 95D cells using 3'-UTR probe by RNA EMSA assay. At the same time, this band could be competed with the unlabled probe. There was also no band obsearved using 5'-UTR probe in both cells.

为了寻找可能与3'-UTR结合的蛋白质反式作用因子，用RNA凝胶阻滞(electrophoresis mobility shift assay，EMSA)的方法，我们在H〓细胞的胞浆蛋白质提取物与3'-UTR探针的结合反应中，发现了一条RNA-蛋白质的结合带，而在95D细胞没有结合带出现；而且这种结合带能被未标记探针竞争性抑制；5'-UTR的结合反应也没有观测到结合带的形成。

Lastly, specimens contain HPV16 DNA were digested with restricted enyzme BamHI, and done with southern transprint to hybridize with digoxigenin-labeled probe of HPV16 genome through nuclear acid hybridization. Results (1)Among 60 cervial carcinoma specimens,the HPV16 E5 positive rate is 33%(20/60) by PCR, 70%(42/60)、58%（35/60）by nuclear acid dot hybridization with HPV16 E5 probe and HPV16 probe .

结果（1）PCR法 60例宫颈癌组织中HPV16 E5 片段的阳性率为33%（20/60）；（2）斑点杂交法60例宫颈癌组织中HPV16 E5阳性率为70%(42/60)，HPV16阳性率为58%（35/60）；（3）宫颈癌组织中HPV16 E5基因的突变率为40%（8/20），迁移位置不甚一致，随机抽取一例E5变异的PCR阳性产物进行测序分析，结果发现在E5片段中有2处变异， nt3979A→C颠换，nt3988T→C转换，导致E5编码区出现1个氨基酸的有义变异。

This device include: Renishaw's high-speed scanner (CYCLON SERIES2), the scanner can be laser-contact probe and probe each other's advantages, precision laser scanning for 0.05mm, contact scanning probe accuracy Up to 0.02mm.

这方面的设备还包括：英国雷尼绍公司的高速扫描仪（CYCLON SERIES2），该扫描仪可实现激光测头和接触式测头优势互补，激光扫描精度为0.05mm，接触式测头扫描精度达0.02mm。

The ultrasound apparatus is EUB8500, maked by Hitachi, ultrasound probe islinear array probe, 7.5-13.0MHz. After we hocused rats and fixuped them, weexamined rats with probe and forced gently. We controled the strength which keeped3 always and adjust the size of frame, and then observed the color within the frameand blue is electropositive.

将大鼠腹腔麻醉、固定，在超声仪下观察，手持探头轻轻加压，根据仪器指示，使压力始终控制在3左右，调整取样框大小，观察取样框内的颜色变化，蓝色为阳性。

Mask Laser Writer, Probe card ,Reticle Case, Test Equipment for Memory, CMP, E-Beam Inspection,Ion Implantation , Optical Defect Inspection , Optical Probe, Therma Probe,Test Reticle Mask

激光绘图光刻机，光学探针，探针卡，掩膜盒，DRAM测试系统，电子束检测设备，光学缺陷检测设备，热探针，测试掩膜版

Bob Eckert has been chairman and chief executive of Mattel for eight years.

美泰董事长兼首席执行官鲍勃·埃克尔特在任已8年。

The outlook for honors and disgraces carries on and surpasses the traditional outlook for honors and disgraces theoretically, and carries on and develops the theory of construction of socialist mora...

社会主义荣辱观的提出在理论上是对传统荣辱观的一次继承和超越，是对社会主义道德建设理论的继承和发展，在实践上有利于形成良好的社会风尚。