查询词典 primer

The informative loci by each primer or primer pair were used to identify 10 cherry tomato varieties,respectively.

分析单引物的品种鉴别能力发现，RGA和SRAP标记具有较高的鉴别效率。

Fifty-three primer combinations were found to amplify nuclear DNA of Chinese cabbage, properly. They amplified the fragments up to 4 381 with an average of the 83. Among them, 9 markers derived from 9 primer combinations were linked to the clubroot resistance gene.

筛选出的适宜53对引物组合共扩增出4381条带，占总扩增片断的33.50%，平均每对扩增出83条，其中9个组合扩增出与抗根肿病基因连锁标记。

In this study the clubroot resistant CR DH line, susceptible inbred line 94SK of Chinese cabbage, the resistant and susceptible DNA bulks constructed from F2 individuals of CR DH line and 94SK were used to select the adaptive AFLP primer combination. AFLP analysis was performed with 256 Pst Ⅰ/Mse Ⅰ primer combinations.

为了筛选出大白菜适宜的AFLP引物组合，研究以抗根肿病大白菜双单倍体CR Shinki、感病自交系94SK及其后代F2构成的纯合抗病和纯合感病DNA混合池为材料，利用256对Pst Ⅰ/Mse Ⅰ引物组合进行了AFLP分析。

A 1172 bp fragment was detected for Wx-7A gene using dominant marker primer from the Wx-A1 normal wheat, a 593 bp and a 574 bp fragment were detected using codominant marker primer from the Wx-A1α normal and Wx-A1 null wheat, respectively.

STS标记结果中，Wx-7A位点的显性标记引物在野生型中扩增出一条1172bp的特异带，即Wx-A1α基因出现；Wx-7A位点的共显性标记引物在野生型中扩增出一条593bp的特异带，在缺失类型中扩增出一条574bp的特异带。

The products amplified by primer 2 had polymorphism. The sequencing results indicated that there were two nucleotide mutations( C→A and C→T )at exon 1183bp and 336bp of hircine GDF9 gene, but the two mutations did not cause amino acid change. The products amplified by primer 3 had polymorphism.

所设计的6对引物扩增产物中共有3对发现了多态，引物1经SSCP分析虽然没有发现多态但经克隆测序却在外显子1编码区前-4bp处检测到A→C的突变。

Sex of early embryos was determined by PCR using double primers (Y-specific DNA primer BOV-97,157 bp; Bovine α-lactalbumin primer in male and female,109 bp).

利用双引物PCR技术对优质良种牛体外受精的冷冻胚胎进行性别鉴定，并对分割后的半胚分别进行PCR扩增和染色体分析，两种方法的判定结果相吻合。

Based on the principal of PCR-primer design, the usage of two kinds of popular primer-design software was reviewed detailedly, including their merit, demerit and usage skills.

在PCR引物设计原则的基础上，详细介绍了两种常用引物设计软件的基本使用方法，并对其各自的优缺点进行了比较。

A band of 850 bp was generated in the line of non-parthenocarpy by the primer N92 both in the parent and in the DNA pool. Accordingly, it is possible that the primer N92 might be used to phenotyping the trait of cucumber parthenocarpy, and further we calculated the genetic distance between the marker and cucumber parthenocarpy trait that might be 18.9 cM.

用65条ISSR引物进行PCR扩增筛选，其中引物N92在亲本和DNA池之间扩增出一条大小为850bp多态性片段，经F2代群体135个单株验证，该片段稳定出现，在单性结实植株中表现为无带，在非单性结实植株中表现为有带，可以作为鉴别单性结实与非单性结实的标记引物。

Last, I sealed the primer with a primer sealer.

最后，我封锁了引物，以物密封。

Upon initiation of PCR after the denaturation phase, the single-stranded DNA fragments flanked by inverted terminal repeats may form either self-annealing "pan-like" structures (preventing the primer binding to its complementary binding sites and suppressing the PCR) or DNA/primer hybrid structures.

经聚合酶链反应开始后，变性阶段，单链 DNA片段两侧末端反向重复序列可能两种自我退火"泛，如"结构（防止引物结合到其互补性结合位点和制止的PCR ）或DNA /引物混合结构。

Panorama Many people are dancing in a large sitting room.

在一个大客厅里，许多人在跳舞。

We fully understand how a microwave oven cooks food, it is first necessary to talk ...

我们充分理解一个微波炉煮的食物，这是首先要谈。。。