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MATERIALS AND METHODS.: Herein we describe our center's experience with a plasmapheresis-based desensitization protocol for highly sensitized patients. Twenty-nine patients had a positive T-cell or positive B-cell lymphocytotoxicity crossmatch against their donors.

材料和方法：在此介绍我中心的相关经验：对于高致敏患者采用血浆置换为基础的脱敏方法。29名患者淋巴毒交叉配型试验提示存在针对其供者的阳性T细胞或B细胞。

The reactive polymorphonuclear leucocytes and histioˉcytes were positive for TIAI,but not for Granzyme B;The tumor cell pleomorphism and positive rate of Ki67were sigˉnificantly associated with Granzyme B expression.

背景反应性中性粒细胞和组织细胞常表达TIAI，但不表达GranzymeB，肿瘤细胞异型性、Ki67阳性率与GranzymeB表达显著相关。

TGF-β1 took on extensive positive in alveolus epithlia in the control group. The positive cytes of the groups under hyperxia-exposure for 3, 7, 14 day distributed in alveolar epithelium, pulmonary interstitial, bronchoepithlia and macrophage, which were notably enhanced along with the time of hyperxia-exposure.

IFN-γmRNA在高氧3天（0.62+0.49）、7天（0.44+0.53）、14天（0.39+0.27 ）均高于空气组(0.20+0.14)，高氧3天达到高峰水平（P.05），高氧7天后开始下降（P＞0.05），高氧14天下降更为明显（P＞0.05）；TGF-β1的阳性细胞在空气组广泛分布于肺泡上皮，高氧组各期广泛分布于肺泡上皮、肺内间质、支气管上皮、血管内皮和巨噬细胞。

Compared with the control, the lungs of 3-day-hyperxia-exposure group developed into edema, hemorrhage and inflammatory infiltration and this condition aggravated at the 7th day. The pulmonary septum was significantly widened at the 14th day, the interstitial fibrocytes as well as alveolar epithelial cells proliferated obviously, and the tendency of lung fibrosis was also presented. The results mentioned above showed that the modle of hyperoxia lung injury was successfully established, and the acute inflamationm was its early symptom while the fibrosis was its late manifistation. There was no positive cytes of IFN-γ in the lung of nomal rats, The positive cytes of the groups under hyperxia-exposure for 3, 7, 14 day, which climbed to the climax at the 7th day and began to decline from the 14th day, were found in alveolus epithlia,pulmonary interstitial, bronchoepithlia and macrophage.

高氧14天（5.46±0.30）虽有所下降，但同空气组比，差异仍然具有显著性（P.05）；BALF中蛋白含量高氧3天（0.45±0.10）、7天（0.66±0.08）、14天（0.79±0.14）显著高于空气组（0.18±0.08，P值均。05）；肺通透系数高氧3天（6.61±2.05）、7天（10.88±1.54）、14天（12.75±2.20）均明显高于空气组（2.99±1.27，P值均。05）；同时病理组织学改变也表明与空气组比较，高氧3天时肺组织水肿、出血、炎性细胞浸润，7天时进一步加重，14天时肺间隔明显增宽，间质纤维细胞和肺泡上皮细胞明显增生，出现肺纤维化倾向；以上结果均表明我们成功地建立了高氧肺损伤动物模型，且急性炎症反应是其早期表现，纤维化改变是其晚期表现。

Results: The positive results of in-situ hybridization showed that the lung tissues of all cases expressed SARS-CoV RNA, and positive signals displayed in cytoplasms purple-blue,NBP-BCIP.

结果：原位杂交检测显示，8例患者肺组织中都表达SARSCoVRNA，阳性信号位于靶细胞胞浆内，呈紫蓝色。

Results:The positive expression of P53 and c－Myc protein in nuclei was bound up with poor-differentiated tumours;the positive expression of Fas and c－Myc protein in cytoplasms was consistent with well-differentiated tumours.

采用免疫组织化学方法对骨肉瘤、骨化性纤维瘤、骨纤维结构不良和正常骨痂组织进行c－Myc、Fas和P53蛋白测定，与肿瘤组织学分级、细胞分化及核丝分裂比较分析。

Results: The positive results of in-situ hybridization showed that the lung tissues of all cases expressed SARS-CoV RNA, and positive signals displayed in cytoplasms （purple-blue, NBP-BCIP.

原位杂交和免疫组化结果显示：支气管上皮细胞、Ⅱ型肺泡上皮细胞、血管内皮细胞、巨噬细胞、纤维母细胞及T淋巴细胞在所有SARS病例中都受到了病毒感染。

Results The expression of Foxo3a, Bim, FasL in oocyte nests and primordial follicles were detected in the nuclei and cytoplasms of some oocytes. The patterns of Bim and FasL positive rates were consistent with that of Foxo3a and TUNEL positive oocytes rates, which reached the peak in the ovaries of 2-day-old rats.

结果 免疫组化显示Bim和FasL都在新生大鼠部分卵母细胞巢和原始卵泡的卵母细胞核内和胞浆高表达，且阳性率曲线都在2d达到高峰，随后逐渐降低，与TUNEL和FoXo3a的阳性率变化曲线一致。

Using Renalin100 to disinfect the reused dialyzer and Renatro PA 100 to reuse the dialyzers, using the dialyzers of anti-HCV IgM positive patients once, reusing the dialyzers of anti-HCVIgG positive patients seperately with those of anti-HCVIgG negative patients, could prevent HCV infection. The way is safe and effective.

应用Renalin100作为复用透析器的杀菌剂，采用Renatro PA 100全自动复用机进行透析器复用，HCVIgM阳性患者透析器一次性应用，HCVIgG阳性透析器复用，但与阴性患者透析器分机复用处理和隔离储存，对防止血液净化中心的复用透析器患者HCV交叉感染有效。

Methods Dialyzers were reused with Renalin 100 as disinfectant and Renatro PA 100 The dialyzers of HCV Igm positive patients were for single use.Dialyzers of anti HW IgG positive patients were reused and stored seperately with those of anti-HW IgG negative patients.

应用Renalin100作为复用透析器的杀菌剂，采用Renatro PA 100全自动复用机进行透析器复用，HCVIgM阳性患者透析器一次性应用，HCVIgG阳性透析器复用，但与阴性患者透析器分机复用处理和隔离储存。

It also analyzes the feature and development of semi-finish grinding and finish grinding by roller press.

同时进行了辊压机半终粉磨和终粉磨系统的特点和发展分析。

When I got there, water was dripping slowly from the ceiling and forming a pool on the floor.

当我到那里的时候，水是慢慢地从天花板滴而且形成在地板上的一个池。