查询词典 phylogenetic development

The genetic relationships among Euglena strains remain poorly understood, despite a rich history of morphological examination and an extensive phylogenetic analysis of the ribosomal RNA genes.

尽管以前对眼虫进行过大量的形态发育研究和基于核糖体RNA基因的系统发育分析，但对于株系之间的关系仍然知之甚少。

In animals, mitochondrial DNA is generally a 15-22 kb circular genome containing 37 genes: 13 protein-coding genes, two rRNA genes, and 22 tRNA genes, as well as a control region. All 37 genes are arranged in the same relative order almost in all vertebrate species from teleost fishes to eutherian mammals. In recent years, mtDNA has been widely used as a useful marker system in numerous phylogenetic analyses in vertebrate relationships.

脊椎动物线粒体DNA是一个环形结构，长度约为15-22kb.mtDNA呈母系遗传，具有相对保守性，已被广泛应用于分析动物个体鉴别和动物类群间的系统发生关系。

Genetic diversity and phylogenetic relationship in goldfish were examined using mitochondrial D-Loop partial sequences at eight representative varieties of goldfishes and wild crucian carp. The eight representative varieties were Red Common Goldfish, Red Fantail, White Oranda with Red Carp, White Pearlscale, Red Tigerhead, Black Moor, Red Moor with Butterfly Tail and Red Bubble-eye with Dorsal Fin. 699 base-pair nucleotide sequences of Mitochondrial D-Loop were examined and analyzed for genetic polymorphism, Sequences data showed that all 129 sequences were grouped into 17 haplotypes. Nine representative varieties shared same haplotype. Five representative varieties of goldfishes had haplotypes respectively.

采用PCR技术扩增了红草金鱼，红文鱼，红顶白高头，白珍珠，红狮头，红龙睛蝶尾，墨龙睛，红水泡八个品种金鱼和野生鲫线粒体DNA的D-Loop区部分序列，PCR产物经纯化、测序、同源序列比对后获得长度为699bp的核苷酸序列，在129个样本中共检测到了17个单倍型，9个品种共享1种单倍型，5个品种的金鱼(BM， MB， WR， RF， RC)检测到各自独有的单倍型。

This was the first report on the phylogenetic characterization of the symbiotic bacteria associated with the symbiotic flagellate Pyrsonympha vertens in the gut of Reticulitermes flavipes.

根据测序克隆的RFLP图谱所代表的克隆数，说明"白蚁菌群1"和CFB phylum的拟杆菌均为与鞭毛虫P.vertens相关的优势共生细菌类群。

The multiple alignment and phylogenetic analysis demonstrated that each genewas a member of a multigene family. 3. The function of 3GT gene was confirmed by Agrobacterium-mediatedtransformation of arabidopsis thaliana. Arabidopsis thaliana was transformed by floral dip method with AgrobacteriumGV3101 carrying expression vector pG3GT. Four transformants were selected for theirgrowth ability on 1/2MS medium containing 50mg/L kanamycin.

马铃薯野生种3GT基因的功能验证为了验证马铃薯野生种3GT基因的功能，构建带CaMV 35S启动子的表达载体pG3GT，转化农杆菌GV3101，用茵液浸泡花序法对拟南芥进行遗传转化，在含50mg／LKan的1／2MS培养基上筛选，得到4个抗性幼苗，转化率为0.13％，对移栽成活的3株进行PCR检测为阳性，Southern blot分析2株表现为阳性并为单拷贝整合。

We can predict the structure and function of new sequence, and analyze the homologous relationship by aligning sequences and constructing phylogenetic tree.

通过多序列比对和系统发育可以预测新序列的结构和功能，分析序列之间的同源关系。

The result was basically consistent with three methods which was NJ, ME, UPGMA constructed in molecular phylogenetic tree, the gayal was clustered together with cow firstly, chimpanzee, rhesus monkey and human clustered together, then together with each other, followed by other species.

用NJ、ME和UPGMA等3种方法聚类构建的分子系统进化树表明，3种方法的聚类结果基本一致，即大额牛与普通牛首先聚为一类，人、黑猩猩、恒河猴也先聚为一类，这两类相聚后再依次同其他物种聚在一起。

Phylogenetic analysis revealed that there exists at least 2 genetical distinct hantaviruses lineages.

结果 选用50个随机引物进行扩增，有5个引物获得清晰、多态性高的RAPD谱带。

The phylogenetic analysis of 22 ND4L sequences of Phocidae from GenBank showed that there are two clades (which belong to Northern Group and Southern Group) supported by relatively high bootstrap value and there are more closed relationship between spotted seal and harbor seal.17 haplotypes with 717bp were obtained from the mtDNA control region, adjacent proline and a portion of the threonine tRNA genes of 46 individuals.

利用从GenBank中下载的22个海豹科动物的ND4L基因序列的系统进化分析表明，海豹科动物分为南半球和北半球两大类群，斑海豹与港海豹为北半球种类，亲缘关系最近。从46个辽东湾斑海豹线粒体DNA控制区和苏氨酸及脯氨酸tRNA基因717bp片段的测序，得到17个单倍型。

SSRs are very suitable for research on genetic diversity and phylogenetic relationship since they are more reliable, co-dominant in heritance large number of alleles per locus, and abundance in genomes.

对椰子SSR反应体系进行了优化，确立了最适宜的体系：在20μL反应体系中，Mg2+、引物和dNTP的最适浓度分别为2.5mmol/L、0.3μmol/L、0.2mmol/L；TaqDNA聚合的最佳使用量为1U，模板DNA应加入50ng，引物最佳退火温度比每对引物Tm值较小者小2-3℃。

The two-acre Dunbar Community Garden in Little Rock has served as a model for several years.

几年来，坐落于小石城，占地两英亩的邓巴社区菜园树立了一个标杆。

Jerry points to the jacket hanging in the bathroom. It's ruined, and unwearable What?

杰瑞指着洗手间里挂着的夹克，毁了，不能穿了）什么？