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The N gene ORF was then subcloned into pET-30a vector and the recombinant plasmid was transformed into E.coli BL21 (DE3) and induced with IPTG. The protein expression was determined by SDS-PAGE. The expressed protein had a molecular weight of 54.4 Ku that existed as inclusion body. Thin-layer scanning showed that the expression product accounted for 30.5% of the total bacterial proteins. The recombinant protein possessed native biological activity and could react with anti-PEDV hyperimmune serum in Western blot.

以阳性质粒为模板，用分别含有BamH Ⅰ和Xho Ⅰ酶切位点的上、下游引物扩增得到ORF，其PCR产物经BamH Ⅰ和Xho Ⅰ双酶切后定向克隆到pET-30a载体，构建的重组质粒命名为pET-30a-PN；将pET-30a-PN转化到大肠杆菌BL21 (DF3)中，在IPTG诱导下进行表达；SDS-PAGE结果表明表达出与预期大小相符的约54.4 Ku的重组蛋白，重组蛋白以包涵体形式存在；薄层扫描结果表明表达产物占菌体总蛋白的30.5%；Western blot分析表明表达的重组蛋白能与抗PEDV高免血清反应，说明该重组蛋白具有免疫学活性。

Study on the Percolation Conductive Behavior of the Conductive Composites Using classic statistic percolation theory, the conductivity behaviors of the PET/EG、PA6/EG and PA6/PET/EG composites were investigated, and the percolation thresholds and critical exponents were obtain. The nonuniversal critical exponent should be attributed to the anisotropy of conductivity, the tunneling conduction, the particular structure, the anomaly distributing of the particles and so on.

四、聚合物—石墨导电复合材料的逾渗导电行为运用统计逾渗理论探讨了PET／EG、PA6／EG及PA6／PET／EG导电复合材料的导电机理，得出了各体系的逾渗值和临界指数，并从导电性能的各向异性、逾渗值以上的隧道导电机理、独特的形态结构、导电粒子的不规则分布等方面分析了造成临界指数非普适行为的可能原因。

Using 11C--PK11195 positron emission tomography, we investigated microglial activation in HD presymptomatic gene carriers, its relationship with striatal neuronal dysfunction measured with 11C-raclopride PET, and the role of PK PET as a possible marker of subclinical disease progression in PGCs. Eleven HD PGCs underwent PK and RAC PET. Their results were compared with those of healthy controls.

利用11C--PK11195电子发射断层扫描，我们研究了HD的基因携带者症状发生前小神经胶质细胞的活化情况，它与纹状体的神经元的机能障碍的关系，以及PET可能作为PGCs亚临床症状进展的指标。11位HDPGCs接受了PK和RACPET检查，其结果与健康对照人群进行比较。

To study these mechanisms, non-manifesting DYT1 mutation carriers (nmDYT1) and presymptomatic HD gene carriers, as well as age-matched controls were scanned with FDG PET in the resting state, and 15O-labeled water PET while performing a motor sequence learning task and motor execution task.

为 了研究这种机制，我们利用氟去氧葡萄糖 PET 扫描技术和 15O 标记水 PET 技术，对非显性 DYT1 突变携带者( nmDYT1)、未发生临床症状的 HD 基因携带者以及同龄对照在静息期和执行运动顺序学习任务以及运动原执行任务期间进行检测。

Methods: Total RNA was extracted from esophageal carcinoma tissue, the primer containing special enzyme was designed, NY-ESO-1 fragment was amplified by RT-PCR, and then was cloned into the expression vector pET-15b by LP Recco PCR Cloning. The recombinants plasmid pET-15b-NY-ESO-1 were identified by restriction endonucleases digest analysis and sequence analysis.

从人新鲜的食管癌组织中提取总RNA，通过RT-PCR技术扩增NY-ES0-1基因3'端的340bp片段，采用靶向克隆法将目的基因插入到原核表达载体pET-15b中，得到重组表达质粒pET-15b-NY-ESO-1，经过筛选，挑选出阳性克隆进行XhoⅠ和BamHⅠ双酶切图谱分析、PCR检测和扩增产物核苷酸序列分析等，鉴定所构建的原核表达载体。

The cold crystallization of uniaxially oriented PET fibers was investigated. The preferred condition for preparation of uniaxially oriented amorphous PET fibers was explored in terms of the draw ratio of as-spun PET fibers at different temperatures.

研究了单轴取向PET纤维的冷结晶过程，对出现的多重结晶峰进行了参数解析，得到了Avrami指数、结晶扩散活化能等结晶动力学参数。

It was demonstrated that some level of order exists in glassy poly by depolarized light intensity technique, but this order is different from that in crystalline PET. The degree of order in glassy PET decrease with the increasing temperature between 15～70℃.

首次用解偏振光法证实了玻璃态PET中一定程度有序性的存在，但它有别于晶态PET中的有序，在15～70℃之间，其有序程度随温度的升高而下降，并与温度之间有着一一对应关系，红外分析表明，该有序仍由含反式亚甲基的链段组成。

The problems of using steel strap in bale packaging in cotton industry in China were analyzed. The reasons and innovative ways of PET substituting for steel strap were put forward to improve the economic efficiency. The characteristics and parameters of applying PET in bale packaging were further analyzed.

目前棉花行业仍普遍采用传统的钢丝捆扎棉花打包的方法，该方法存在很多问题，提出新型有效和经济的PET塑钢带替代钢丝打包的多种理由，并对PET塑钢带对棉花捆扎打包的技术特点和有关参数作了进一步分析。

100KW factory specializing in the production of the following high-frequency machines, leading products: Automatic Blister molding machine, whole plant equipment ,5-30KW automatic synchronization slip Units fuse machine, double-headed synchronous machine fuse ,4-100KW automatic sliding Units welder, 4-15KW single turntable welder ,2-15KW welder-type double-headed foot, 8-25KW welder canvas ,4-15KW shielded single slip Units, rotary high frequency welding machine. 8-15KW environmental protection welder dedicated PET material, PP, PET, PVC plastic fuse box dedicated machines, ultrasonic plastic welding machine and so on.

本厂专业生产100KW以下高周波机器，主导产品：全自动吸塑成型机、整厂设备，5-30KW全自动滑台同步熔断机、双头同步熔断机、4-100KW全自动滑台熔接机，4-15KW 单头转盘熔接机，2-15KW双头脚踏式熔接机， 8-25KW帆布熔接机，4-15KW屏蔽式单头滑台、转盘式高周波熔接机。8-15KW环保料PET专用熔接机，PP、PET、PVC胶盒专用熔断机，超声波塑焊机等。

Special line: PET bottles to send the wind line, PET bottles transmission line, eight-treasure Li transportation, storage assembly line speed chain, refrigerator-line detection of, stainless steel transmission lines around with CPT, conveyor Store line, groove belt, transportation of finished Line, the size and quality control systems, improve water vat, with tailgate climbing belt, with the assembly line stands, the knife-edge transition belt turn, to roll lines, cell delivery systems, computer assembly lines, power assembly lines, power lines Raceway , To send the wind-line, high-speed sorting lines, transmission lines Raceway, drum line, after the assembly lines overhang, the chemical industry transmission lines, aging transmission lines, Tetra transmission lines, transmission lines plate chain, chain lost keel box line, spiral Chain plate line, spiral belt, a motorcycle transmission lines, motorcycle assembly line, climbing belt, a belt transmission line group, bottles of beer delivery systems, aerodynamic balance suspended, the lines between the pneumatic hoist, automotive transmission lines, light-top Transfer or devices, thermal plastic packet transmission line, stretching line wheel, food delivery lines, transmission lines, transmission lines climbing boxes, fruit cleansing transmission lines, the level of circulating conveyor chain, no power lines Raceway, no power telescopic Raceway lines, lines between the hoist, the letter-box sorting systems, conveyor suspension, tobacco conveyor, the parcel sorting conveyor systems, packet transmission lines, packet transmission line combinations, straight plate section, gravity-shelf access device , Heavy-duty power lines Raceway, heavy-duty conveyor chain, chain of beads to lose face envelope, turning plate, vehicle loading and unloading conveyor, the assembly line, assembly line, automatic transmission storage systems, feeding

特殊流水线：PET瓶风送线，PET瓶输送线，八宝粥输送骊，倍速链储装配线，冰箱检测线，不锈钢输送线，彩管绕带机，仓储输送线，槽型皮带机，成品输送线，尺寸与质量检测系统，大桶水提升设备，带挡板爬坡皮带机，带灯架装配线，刀口过渡转弯皮带机，地滚线，电池输送系统，电脑装配线，动力装配线，动力滚道线，风送线，高速分拣线，滚道线输送系统，滚筒线，后悬装配线，化工行业输送线，老化输送线，利乐包输送线，链板输送线，龙骨链输箱线，螺旋链板线，螺旋皮带机，摩托车输送线，摩托车装配线，爬坡皮带机，皮带输送线组，啤酒瓶输送系统，气动平衡吊，气动线间提升机，汽车输送线，轻型顶升移载装置，热塑膜包输送线，伸缩滚轮线，食品输送线，输送线，输箱爬坡线，水果输送清洗线，水平循环链式输送机，无动力滚道线，无动力伸缩滚道线，线间提升机，信盒分拣系统，悬挂输送机，烟草输送机，邮包输送分拣系统，邮包输送线，邮包组合输送线，直段板式机，重力式货架存取装置，重型动力滚道线，重型链式输送机，珠面链输包线，转弯板式机，装卸车输送机，装配流水线，装配线，自动仓库输送系统，上料机

I'm strongly against the death penalty — it's an eye for an eye.

我不赞成死刑——这是以牙还牙的报复行为。

And to get you the support you need, we're enlisting all elements of our national power: our diplomacy and development, our economic might and our moral suasion, so that you and the rest of our military do not bear the burden of our security alone.

并给你们所须的支援，我们正徵召国家所有各种的力量：我们的外交及发展，我们的经济力量与道德劝说，所以你们与其他军人不须要孤独地负起国家安全的责任。