查询词典 passaged

The shortage of this way was that feeder cells could be passaged together with hESCConclusion HAFi could replace MEF as feeder layer to support the long-term culture oftwo Chinese human embryonic stem cell lines. This could remove the cells of animal originand heterogenetic pollution in the culture system. It also reduced the culture of primarycells and solved the instability caused by different batches of primary cells.

结论HAFi细胞可以用做两株中国人胚胎干细胞培养的饲养层，取代MEF后解决了动物源性细胞混杂和异源污染问题，省却了饲养层原代细胞的培养步骤同时解决了由不同批次饲养层差异带来的培养体系的不稳定。

The primary cultured fetal mesencephalic neural stem cells are passaged every 7days.

将人胚中脑来源的神经干细胞体外长期培养，每隔5-7天传代一次。

METHODS: Rat cerebral cortex was obtained sterilely, made into monoplast suspension by the mechanical separation, and then incubated in the serum-free MEM/F12 medium, supplemented with B27 neurotrophic additive for 1 week. Subsequently, cells were passaged.

无菌条件下取胎鼠大脑皮质，剪碎后机械分离法制作单细胞悬液，加入含B27神经营养添加剂的无血清MEM/F12培养基进行培养，1周后传代。

RESULT:①Morphologic character of ADSCs: The primary ADSCs presented in long or short spindle-shape or multangular shape, but the third passaged ADSCs and their offspring indicated uniform long spindle-shape.

结果：①脂肪间充质干细胞的形态特征：原代脂肪间充质干细胞呈短梭形、梭形及多角形，3代后呈均一长梭形。

An HIV-1 strain, SH01, isolated by coculture of peripheral blood mononuclear cells was easily passaged in MT4 cell line and multinuclear giant syncytia could be induced in MT2 cells.

用外周血单个核细胞注意混合培养法分离到的HIV-1 SH01株，具有典型的HIV颗粒的形态学特征，核心颗粒呈锥形，可见芽生释放的全过程。

Results The hEFs were successfully isolated and cultivated from gonadal ridges and dorsal mesenteries of human embryos. They could be passaged beyond the 25th generation. The biologic characteristics of the cells did not change, even in high-passage cells or frozen-thawed cells. The cells expressed prolyl 4-hydroxylase β, but not cytokeratin-4, which was similar to the fibroblasts. The cultured cells expressed bFGF and LIF.

结果：从人胚胎生殖腺嵴和肠背系膜中成功地分离培养出hEFs，该细胞可传25代以上，且经过传代及冻存复苏后生物学特性无改变；hEFs表达脯氨酰4-羟化酶β亚单位，不表达细胞角蛋白4，确定其为成纤维细胞；该成纤维细胞表达bFGF和LIF。

Methods: The fibroblasts companying human hepatoma were primarily cultured with explant culture method, first passaged after they spread to the bottom of the culture bottle, and pured by enzyme digestion and repeated strapping the cells were identificated through observing the morphologic change using invert microscope and detecting expression of vimentin, keratin by iMmunohistochemical method.

应用组织块培养法进行人肝癌伴生成纤维细胞的原代培养，细胞铺满培养瓶底后首次传代，通过胰酶消化法和反复贴壁法进行成纤维细胞的纯化；通过倒置显微镜观察细胞形态、免疫组织化学方法检测细胞膜波形蛋白、角蛋白表达情况，对细胞进行鉴定。

Results After six passages in BHK-21 cells, cytopathic effect was observed by microscopy. The rescued virus was rejuvenated once in unweaned mice, and then came back to cell passage. We found that the poly tract of rescued virus was shortened when the virus was passaged twice in BHK-21 cells. Although the data of LD50 in mice and TCID50 in BHK-21 cells showed that the virulence and infectivity of genetic engineering viruses were lower than its parental virus, no significant difference was observed between the genetic engineering viruses with the different length poly tract.

我们发现，基因工程毒在BHK-21上连续传代至第6代时，可见明显的致细胞病变效应(cytopathic effect，CPE)；在经过一代乳鼠接种之后再重新适应到BHK-21细胞的过程中，poly区段出现了缩短现象；乳鼠致病性试验和BHK-21细胞TCID50测定结果表明，尽管基因工程毒与母本毒相比毒力偏低，但含有不同长度poly的基因工程毒之间并无显著差异。

RESULTS: The passaged MSCs exhibited uniform appearance in fusiform shape, and their growth was slowed down after 9 passages, exhibiting cytoplasm vacuolization and body enlarging.

结果：传代培养的骨髓间充质干细胞形态均一，呈梭形、纺锤形，克隆样生长，培养至9代后生长减慢，并出现胞浆空泡化、胞体变大等老化现象。

The full-length genome cDNA containing genetic tags and flanked by Hammerhead ribozyme and Hepatitis delta ribozyme were cloned downstream of the CMV enhancer and the chicken beta actin promoter of the vector pCAGGS. After purified by Plasmid Midi Kit, the recombinant infectious clones named pCAGGmGtAHRT and pCAGGmGtBHRT were directly co-transfected DFI cells indued by Lipofectamine~ 2000. 72h post transfection. the supernatant of cell cuture was harvested and continue passaged in CEF cell. The results of indirect immunofluorescence.

分别在Gt株基因组两端引入了锤头状核酶结构(Hammerhead ribozyme，HamRz)和丁肝病毒核酶结构(Hepatitis delta ribozyme，HdvRz)，并将其克隆在真核表达载体pCAGGS的CMV增强子和β肌动蛋白启动子下游，构建IBDV感染性克隆pCAGGmGtAHRT和pCAGGmGtBHRT，经Plasmid Midi Kit纯化后，在Lipofectamine~ 2000的介导下，直接转染DFI细胞，72h后取上清继续在CEF上传代。

If you were not , and OS X just booted normally, have no fear.

如果你没有，和OS X刚刚启动通常，没有恐惧。

I only want to preserve our all .

我只想保存好我们的一切