查询词典 passaged

Since the first cases in North America found that after the cases are continuously passaged, and the more passaged more.

自北美发现第一例病例以后，病例是不断传代的，而且越传代越多。

A strain of new virus was isolated from fledgling budgerigars by authors in 1995. Cytopathic effect became visible after the isolate was passaged 4 generations on budgerigar embryo fibroblast and 5 generations on Chicken embryo fibroblast.

摘 要 1995年作者从湖北濒死鹦鹉体内分离到一株新病毒，经鹦鹉胚成纤维细胞盲传四代过渡到鸡胚成纤维细胞，再传至第五代出现细胞病变。

The third passage chondrocytes were divided into blank group, different desity PAP groups, different desity glucosaminsalfate groups which were passaged to 4th generation and contrast to the 2nd passage group. The chondrocytes of different groups were detected with the method of histochemistry for S-A-β-gal,and with alcian blue test for the content and constructure of GAG of ECM, immuocytochemistry for type Ⅱcollagen and PCNA, MTT assay for proliferation, RT-PCR for type Ⅱcollagen and Aggrecan, flow cytometry for cell life cycle and proliferation index,by which to observe PAP's function regarding to the appearance and functional status in the process of chondrocyte's cataplasia and senescence.

将P3软骨细胞分为空白对照组、鹿茸多肽不同浓度组、硫酸氨基葡萄糖不同浓度组进行传代培养，同时以P2代软骨细胞为对照组，进行组化检测老化相关β-半乳糖苷酶，阿力新蓝染色检测胞外基质硫酸GAG含量和结构，MTT比色检测增殖，免疫细胞化检测PCNA和Ⅱ型胶原，RT-PCR检测Ⅱ型胶原、Aggrecan蛋白，流式细胞仪分析细胞周期和增殖指数等方法，对鹿茸多肽抗软骨细胞退变老化进行分子生物学研究。4。

The 3rdpassage chondrocytes were divided into blank group, different concentration PAP groups,different concentration glucosaminsalfate groups and were sequently passaged to 4thgeneration. The 2nd passage chondrocytes was contrasted as young cells group. Thechondrocytes of different groups were detected with the methods of histochemistry forS-A-β-gal, and with alcian blue test for the content and constructure of GAG of ECM,immuocytochemistry for typeⅡcollagen and PCNA, MTT assay for proliferation, RT-PCRfor typeⅡcollagen and Aggrecan, flow cytometry for cell life cycle and proliferationindex,by which to observe PAP"s function regarding to the appearance and functional status inthe process of chondrocyte"s cataplasia and senescence.

将P3软骨细胞分为空白对照组、鹿茸多肽不同浓度组、硫酸氨基葡萄糖不同浓度组进行传代培养，同时以P2代软骨细胞为对照组，进行组化检测老化相关β-半乳糖苷酶，阿力新蓝染色检测胞外基质硫酸GAG含量和结构，MTT比色检测增殖，免疫细胞化检测PCNA和Ⅱ型胶原，RT-PCR检测Ⅱ型胶原、Aggrecan蛋白，流式细胞仪分析细胞周期和增殖指数等方法，对鹿茸多肽抗软骨细胞退变老化进行分子生物学研究。4。

The cells were passaged every 5 to 6 days after digestion with 0.25% trypsin when cells were 80% confluent.

待细胞长至80%汇合，用0.25%胰蛋白酶消化传代，每五六天传代1次。

Media were changed every 3 to 4 days. Cells were passaged with ratio of 1: 3 when almost confluent. Passage 3 cells were used for further experiment.

可能是不同组织的微环境中含有不同的因子，促进进入不同组织的MSCs获得靶组织的表型，向不同谱系分化。

METHODS: Femora of two rats were selected and BMCs were passaged using whole bone marrow culture method.

取2只大鼠股骨，全骨髓培养法传代扩增骨髓间充质干细胞。

METHODS: HPMSCs were isolated from placenta of fetation human by density gradient centrifuge. Following bone marrow heparin anticoagulation, human BMSCs were isolated, purified and passaged by density gradient centrifuge.

采用密度梯度离心法从胎盘中分离、纯化和传代培养人胎盘源性间充质干细胞；骨髓肝素抗凝后，采用密度梯度离心法分离、纯化和传代培养人骨髓基质干细胞。

Bone marrow was obtained from healthy adult donors. METHODS: HPMSCs were isolated from placenta of fetation human by density gradient centrifuge. Following bone marrow heparin anticoagulation, human BMSCs were isolated, purified and passaged by density gradient centrifuge.

采用密度梯度离心法从胎盘中分离、纯化和传代培养人胎盘源性间充质干细胞；骨髓肝素抗凝后，采用密度梯度离心法分离、纯化和传代培养人骨髓基质干细胞。

High variability of human cytomegalovirus UL150 open reading frame in low-passaged clinical isolates.

人巨细胞病毒UL145基因在先天感染患儿临床株中的多态性研究。

"Second Life is remarkably easy to work with, and is very popular,"

"第二次生命是显着容易的工作，并且很受欢迎，"

For example, we usually assume that materials are homogeneous and isotropic and free of internal defects or flaws.

为了得到适合有限元分析的模型，我们必须经过如图2所示的简化步骤。