查询词典 normalized vector

Taking the pcDNA 3.1 expressions vector as the frame structure, we try to set up a eukaryon expression vector.

并以pcDNA3.1表达载体为框架结构，尝试构建真核表达载体。

After inserting the mutant fluorescence protein gene into the vector, the novel vaccinia virus vector p16H1FP and p16H2FP to express proteins as fusion products containing an additional amino-terminal stretch of six histidines were constructed.

我们用PCR扩出N-端融合6×His·Tag多克隆位点基因序列，插入p16表达载体，并在其多克隆位点下游装入带有巨细胞病毒启动子的突变型荧光蛋白基因，构建成两个读框的N-端融合6×His·Tag并带有荧光蛋白报告基因的分离型痘苗病毒表达载体。

Returns a vector which lists the same lexemes as the given vector, but which lacks any position or weight information.

返回一个带有一列与给出向量相同语意的向量，但是它去掉了所有的位置和权重信息。

Using plant bivalent expression vector pCAMBIA1300 and pBI121 as basic components, a new binary vector harboring tomato chitinase gene Chi3 and tomato β-1,3-glucanase gene Glu-Ac, was constructed through sequential restriction digests and ligations recombination. The two genes driven by CaMV35s promoter were successfully transferred into watermelon cultivars "ZhongYuYiHao" via Agrobacterium-mediated transformation.

构建了同时含有番茄儿丁质酶基因（Chi3）和β-1，3-葡聚糖酶基因的双价抗真菌基因植物表达载体，以西瓜子叶块为外植体，采用根癌农杆菌介导法，将Chi3和Glu-Ac同时导入西瓜栽培种&中育一号&，共获得46株抗性再生植株。

The deleted mutant PAP gene was also cloned into yeast secreted expression pPIC9K vector to form pPIC9K~3, then the vector was transferred into Pachia pastoris GS115 strain. The specific expression protein was secreted into the medium after inducing with methanol and the protein amount reached about 50-60 U g per millilitre measured by UV-absorbed methods in the supernatant of the medium via high density fermentation. SDS-PAGE results showed that there was one protein band in the gel which molecular weight was about 34Ku . The protein could specific react with France PAP antiserum in Western-blotting. Activity tests revealed t

将缺失型PAP基因克隆于酵母分泌型表达载体PPICgK构成重组载体，然后导入毕赤酵母（P8chia nastoris）菌株GSlls细胞中，在甲醇的诱导下，经过酵母高密度发酵进行PAP的表达，经SDS－PAGE分析，结果表明，在培养基上清液中含有一明显的特异性蛋臼条带，大小为34Ku，经Western－blotting分析，该蛋白与法国PAP抗血清有特异性反应，体外活性检测表明该蛋白对TMV的侵染性具有高度的抑制性，说明该 PAP基因在毕赤酵母 GS中也得到了正确表达。

VITS and VIR are OK also vector mode shows, the method outputs the blanking signal of undee oscillograph the Z input that receives vector oscillograph.

VITS和VIR也能够矢量模式显示，办法将波形示波器的消隐旌旗灯号输出接到矢量示波器的Z输入。

Development of vector resistance to pyrethroid insecticides has been reported all over the world,which is a major obstacle to control of vector-borne diseases.

媒介昆虫的拟除虫菊酯抗性已经在世界范围内存在，是控制媒介传染病的一个主要障碍。

The PTZ plasmid vector was changed to plasmid vector pSP72, which contains both T7 and Sp6 promotors for transcription of antisense (T7) and sense (Sp6) RNA probe in vitro. The results revealed the label rate of cRNA probe is high, the hybrids between RNA-RNA are stable, so the background of hybridization is satisfactory.

本实验将质粒载体PTZ转换为带有T7和Sp6两个启动基因的pSP72载体，故可在体外转录cRNA时同时得到意义（Sp6为启动基因）和反意义（T7为启动基因）探针，实验结果表明，cRNA探针标记率高，杂合物稳定，故所获杂交反应背景较为满意。

The BDDcFⅧ gene was ligated behind PUB and 20H1 promotors to create lentiviral vectors pTK161 and pTK162. Meantime lentiviral vectors pTK161' and pTK162' were produced by cloning a green fluorescent protein into pTK151 and pTK152, which was driven by PUB and 2OH1 promotors respectively. Vector supernatant were prepared by using transfer calcium phosphate mediated-cotransfection of 293T cells. The virus vector,△NRF packaging-plasmid, and VSV-G envelope-plasmid was assayed by titers and cFⅧ activity in cell culture supernatant after infection into 293T cells.

用构建携带cFⅧ基因的慢病毒载体pTK161和pTK162（含20HI启动子），同时构建含绿色荧光蛋白的慢病毒载体pTK161'和pTK162'（含2OH1启动子）的方法，分别与包装质粒△NRF、包膜蛋白质粒VSV-G共转染293T包装细胞，将包装好的病毒颗粒再感染293T细胞，检测病毒滴度和培养细胞上清中cFⅧ活性。

The main purpose of this paper is to propose a unsupervised clustering-based method to pre-extract the support vector for training the SVM in light of the theory that the classification facet of SVM is determined by support vector only. By analyzing the limitation existed in current multi-class classification methods, and taking into account comprehensively the separability of the class set of nodes, a hierarchical classification method of SVM binary tree based on tree classifier with optimized overall performance is designed.

针对SVM的分类面仅由支持向量决定的理论，提出一种基于无监督聚类方法来预抽取支持向量，训练向量机；并分析现有多类分类方法所存在的弊端，基于综合考虑节点的类集合可分性，设计一种基于树分类器整体性能最优的SVM二叉树层次分类方法。

Don not attempt to do something which you can not to do.

不要企图做那些办不到的事情。

The expression of CTGF and TNF-αweredetected by immunochemistry and the number of Clara Cells was calculated.

光镜下观察肺组织的病理变化，采用免疫组化染色观察肺组织中结缔组织生长因子和肿瘤坏死因子-α的表达和Clara细胞的数量。