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To investigate whether the expression of cdc2 and cyclin B1 in spermatogenic cells during spermatogenesis is actually a temperature dependent event, in situ hybridization, Western blotting and immunohistochemistry analysis were used to study the expression of cdc2 and cyclin B1 in normal and cryptorchid testis. Results showed that heat would differentially hurt male germ cells in different developmental stages during spermatogenesis, especially the pachytene primary spermatocytes. Most of spermatogonia in contralateral cryptorchid testis were not harmed fatally by heat as yet, indicating that spermatogonia could resist to beat to a certain extent. In this case spermatogonia could develop to pachytene/diplotene primary spermatocytes, but they could not acquire the ability to complete the transition from mitosis to meiosis, and then appeared to go through apoptosis. Therefore, we could not find the descendants of meiosis: secondary spermatocytes and round spermatids, elongated spermatids and spermatozoon. The abdominal temperature had no significant influence on the transcription of cdc2 and cyclin B1 in the spermatogonia and pachytene/diplotene primary spermatocytes. In normal rabbit testis, cyclin B1 increased in the pachytene/diplotene primary spermatocytes before meiosis and reached its peak in the spermatids.

为了解精子正常发生过程中cdc2和cyclin B1表达的低温依赖性,我们利用原位杂交和免疫组化等方法,研究了正常和隐睾精子发生过程中cdc2和cyclin B1的转录和翻译调控活动,结果表明:(1)热对各阶段的雄性生殖细胞都有损害,粗线期的初级精母细胞尤为敏感,实验性隐睾内的精原细胞尚未完全受到"致命"影响,说明精原细胞对热有一定的耐受性,但即使成为粗线期/双线期初级精母细胞,却未能获得由有丝分裂过渡到减数分裂的能力,呈现不同程度的凋亡,所以在整个切片中找不到源自减数分裂的产物----次级精母细胞、圆形精子细胞,更谈不上长形精子细胞和精子的形成;(2)腹腔高温未明显地影响隐睾精原细胞和粗线期/双线期初级精母细胞中cyclinB1和cdc2的转录,说明高温并不是通过影响cyclin B1和cdc2的转录活动而导致生精过程阻断的;(3)正常兔睾丸组织中,〓在精原细胞和粗线期/双线期精母细胞中均有表达:cyclin B1蛋白在减数分裂前期的粗线期/双线期初级精母细胞中的表达量增加,于变态末期的精子细胞中达高峰。

Chapter 2 introduces some propaedeutics of classic differential geometry, which include the first basic form, the second basic form, normal curvature, main curvature, mean curvature, Gauss curvature, unit normal vector, main vector, etc.

第二章介绍一系列的预备知识,主要是关于古典微分几何中第一,第二基本形式的定义,法曲率,主曲率,平均曲率,Gauss曲率,单位法向量,主方向等的定义。

Let F=LF be a s-closed local formation such that every minimal non-F-groups is solvable. It is proved that 1 If every cyclic subgroup of of order 4 and every minimal subgroup of G are contained in, then G is a F-group. 2 If there exists a normal subgroup N of G such that G/N∈F and every cyclic subgroup of N of order 4 is weakly c-normal in G and every element of N of prime order is contained in Z(superscript f subscript ∞), then G is a F-group.

设F=LF是一个子群闭的局部群系,满足每个极小非F-群是可解群,证明了:1如果G的任意极小子群和任意4阶循环子群都含于Z中,那么G是F-群;2如果存在G的正规子群,使得G/N∈F,且N的任一4阶循环子群在G中弱c-正规,N的任一素数阶元含于Z中,那么G是F-群。

Results Intraocular pressure recovered to normal in 15 cases with in 3 days and 1 case with in 3 weeks, 2 cases remained under normal after the surgery. 16 cases experienced improvement of vision after surgery. cystoid macular edemaoccurred to two cases.

结果 15例在术后3 d内眼压恢复正常及短暂增高后恢复正常,1例在术后3周内眼压逐渐恢复正常,2例手术后眼压仍然低。16例术后视力不同程度提高。2例出现黄斑囊样水肿。

The rate of appearance of normal finger prints in various nationalities and regions has obvious difference, the normal rating of the dactylogram of the juveniles of the She Nationality differs from that of the local juveniles of the Han nationality.

不同地区不同民族的正常指纹出现率存在明显差异,畲族青少年指纹正常值与当地汉族指纹存在差异。

Everybody was decompensate, without ALT level must be treated, and you should given the kidney function, if the kidney function is normal, normal dose of the anti-viral drugs.

如果病人的肾功能正常,给予正常剂量的抗病毒药物。

General assumptions probability density function is normal, because normal mathematical better foundation.

一般假设概率密度函数是正态分布,因为正态分布数学基础较好。

Rat models of acute blood stasis, hair depilation and qi-deficiency was respectively established, and in each group,the rats were again divided into 3 subgroups which were subjected respectively to treatment with Shengfaling at normal con-centration, Shengfaling at half the normal concentration or with 65% ethanol as the control.

分别建立大鼠急性血瘀、脱毛、气虚模型,用正常浓度、1/2浓度生发灵酊剂和空臼对照液3种药物分别进行处理,检测各有关指标。

Methods A total RNA from ten cases of ovarian cancer and contral normal ovarian tissues were extracted by a single step method. The cDNA retro-transcribed from equal quantity mRNA derived from ten cases of ovarian carcinoma and normal ovarian tissues, which were labeled cDNA strand with Cy5 and Cy3 fluorescence as probes.

按一步法分别抽提10例人卵巢癌组织和对照正常卵巢组织的总RNA并纯化mRNA;分别从10例取等量的人卵巢癌组织和对照组织的mRNA分别混合后逆转录合成以Cy5和Cy3标记的cDNA一链做探针,混合后在含有8464点人类体细胞基因模板的表达谱cDNA芯片上进行杂交。

The candidates should be aged below 22, who apply for Dance Departments of Beijing Normal University and Inner Mongolia Normal University, Acting Department of Donghua University DHU

北京师范大学舞蹈学专业、内蒙古师范大学舞蹈学专业、东华大学表演专业要求考生年龄在22周岁以下。

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推荐网络例句

Don not attempt to do something which you can not to do.

不要企图做那些办不到的事情。

The expression of CTGF and TNF-αweredetected by immunochemistry and the number of Clara Cells was calculated.

光镜下观察肺组织的病理变化,采用免疫组化染色观察肺组织中结缔组织生长因子和肿瘤坏死因子-α的表达和Clara细胞的数量。

The latest results are published online January 13 in the Journal of the National Cancer Institute. They come from a case–control study that involved 459 cases,"which, for the rarity of this cancer, is a very big study," Dr. Stang said.

Stang医师表示,这项最新的研究结果线上发表在1月13日的国家癌症机构期刊上,研究来自一项收纳459个病例的病例控制研究,这是一项非常大的研究。