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The effects and mechanism of GABAergic neurons, NOergic neurons, opioid peptide and cyclic adenosine monophosphate in the nucleus reticularis thalami on sleep-wakefulness cycle of rats and the effects and mechanism of the 5-HTergic nerve fibers project from the nucleus raphes dorsalis to RT on sleep-wakefulness cycle of rats were investigated with the methods of brain stereotaxic, nucleus spile, microinjection and polysomngraphy.1. The effects of GABAergic neurons in RT on sleep-wakefulness cycle of rats1.1 Microinjection of 3-mercaptopropionic acid (3-MP, a kind of glutamate decarboxylase inhibitor) into RT. On the day of microinjection, sleep only decreased a litter. On the second day, sleep marked decreased and wakefulness marked increased. On the third and fourth day, sleep and wakefulness stages resumed to normal.1.2 Microinjection of gamma-amino butyric acid (GABA 1.0μg) into RT enhanced sleep and reduced wakefulness compared with control; while microinjection of L-glutamate (L-Glu, 0.2μg) decreased sleep and increased wakefulness; microinjection of bicuculline (BIC, 1.0μg), a GABAA receptor antagonist, enhanced wakefulness and reduced sleep; microinjection of baclofen (BAC, 1.0μg), GABAB receptor agonist, had the same effects as GABA.2. The effects of NOergic neurons in RT on sleep-wakefulness cycle of rats2.1 Microinjection of L-arginine (L-Arg, 0.5μg) into RT decreased sleep compared with control, but there were on statistaical difference between L-Arg group and control; while microinjection of sodium nitroprusside (SNP, 0.2μg), a NO donor into RT, sleep marked decreased and wakefulness marked increased. Microinjection of nitric oxide synthase inhibitor, N-nitro-L-arginine (L-NNA, 2.0μg) into RT enhanced sleep and reduced wakefulness.2.2 After simultaneous microinjection of L-NNA (2.0μg) and SNP (0.2μg) into RT, SNP abolished the sleep-promoting effect of L-NNA compared with L-NNA group; after simultaneous microinjection of L-NNA (2.0μg) and L-Arg(0.5μg) into RT, we found that L-NNA could not blocked the wakefulness-promoting effect of L-Arg.3. The effects of opioid peptide in RT on sleep-wakefulness cycle of rats3.1 Microinjection of morphine sulfate (MOR, 1.0μg) into RT increased wakefulness and decreased sleep compared with control; while microinjection of naloxone hydrochloride (NAL, 1.0μg), the antagonist of opiate receptors, into RT, enhanced sleep and reduced wakefulness.3.2 After simultaneous microinjection of MOR (1.0μg) and NAL (1.0μg) into RT, the wakefulness-promoting effect of MOR and the sleep-promoting effect of NAL were not observed compared with control.4. The effects of cAMP in RT on sleep-wakefulness cycle of rats Microinjection of cAMP (1.0μg) into RT increased sleep and decreased wakefulness compared with control; microinjection of methylene blue (MB,1.0μg) into RT enhanced sleep and reduced wakefulness compared with control.5. The effects of the 5-HTergic nerve fibers project from DRN to RT on sleep-wakefulness cycle of rats5.1 When L-Glu (0.2μg) was microinjected into DRN and normal sodium (NS,1.0μg) was microinjected into bilateral RT. We found that sleep was decreased and wakefulness was increased compared with control; when L-Glu (0.2μg) was microinjected into DRN and methysergide (MS,1.0μg), a non-selective 5-HT antagonist, was microinjected into bilateral RT, We found that sleep was enhanced and wakefulness was reduced compared with L-Glu group.5.2 When p-chlorophenylalanine (PCPA, 10μg) was microinjected into DRN and NS (1.0μg) was microinjected into bilateral RT, We found that sleep was increased and wakefulness was decreased compared with control; microinjection of 5-hydroxytryptaphan (5-HTP, 1.0μg), which can convert to 5-HT by the enzyme tryptophane hydroxylase and enhance 5-HT into bilateral RT, could block the effect of microinjection of PCPA into DRN on sleep-wakefulness cycle.
本研究采用脑立体定位、核团插管、微量注射、多导睡眠描记等方法,研究丘脑网状核(nucleus reticularis thalami,RT)中γ-氨基丁酸(gamma-amino butyric acid ,GABA)能神经元、一氧化氮(nitrogen monoxidum,NO)能神经元、阿片肽类神经递质、环一磷酸腺苷(cyclic adenosine monophosphate,cAMP)及中缝背核(nucleus raphes dorsalis,DRN)至RT的5-羟色胺(5-hydroxytryptamine,5-HT)能神经纤维投射对大鼠睡眠-觉醒周期的影响及其作用机制。1 RT内GABA能神经元对大鼠睡眠-觉醒周期的影响1.1大鼠RT内微量注射GABA合成关键酶抑制剂3-巯基丙酸(3-MP,5μg),注射当天睡眠时间略有减少,第二日睡眠时间显著减少,觉醒时间明显增多,第三、四日睡眠和觉醒时间逐渐恢复至正常。1.2大鼠RT内微量注射GABA受体激动剂GABA( 1.0μg)后,与生理盐水组比较,睡眠时间增加,觉醒时间减少;而RT内微量注射L-谷氨酸(glutamic acid, L-Glu, 0.2μg)后,睡眠时间减少,觉醒时间增加;RT内微量注射GABAA受体阻断剂荷包牡丹碱(bicuculline,BIC,1.0μg)后,睡眠时间减少,觉醒时间增加;RT内微量注射GABAB受体激动剂氯苯氨丁酸(baclofen,BAC,1.0μg)后,产生了与GABA相似的促睡眠效果。2 RT内NO能神经元对大鼠睡眠-觉醒周期的影响2.1大鼠RT内微量注射NO的前体L-精氨酸(L-Arg,0.5μg)后,与生理盐水组对比,睡眠时间略有减少,但无显著性意义;而RT内微量注射NO的供体硝普钠(Sodium Nitroprusside,SNP,0.2μg)后可明显增加觉醒时间,缩短睡眠时间;微量注射一氧化氮合酶抑制剂L-硝基精氨酸(L-arginine,L-NNA,2.0μg)后,引起睡眠时间增多,觉醒时间减少。2.2大鼠RT内同时微量注射L-NNA(2.0μg)和SNP(0.2μg)后与L-NNA组比较发现SNP逆转了L-NNA的促睡眠作用;RT内同时微量注射L-NNA(2.0μg)和L-Arg(0.5μg)后,与L-NNA(2.0μg)组比较发现L-Arg可以增加觉醒而缩短睡眠,其促觉醒作用未能被NOS的抑制剂L-NNA所逆转。3 RT内阿片肽对大鼠睡眠-觉醒周期的影响3.1大鼠RT内微量注射硫酸吗啡(morphine sulfate,MOR,1.0μg)后与生理盐水组对比,睡眠时间减少而觉醒时间增加; RT内微量注射阿片肽受体拮抗剂盐酸纳洛酮(naloxone hydrochloride,NAL,1.0μg)后与生理盐水组比较,睡眠时间增加而觉醒时间减少。3.2大鼠RT内同时微量注射MOR(1.0μg)和NAL(1.0μg)后,与生理盐水组对比,原有的MOR促觉醒效果和NAL的促睡眠效果都没有表现。4 RT内环一磷酸腺苷信使对大鼠睡眠-觉醒周期的影响大鼠RT内微量注射cAMP(1.0μg)后与NS(1.0μg)组比较,睡眠时间增多而觉醒时间减少;RT内微量注射亚甲蓝(methylene blue,MB,1.0μg)后,与NS组比较,睡眠时间增多而觉醒时间减少。5中缝背核投射到丘脑网状核的5-羟色胺能神经纤维对大鼠睡眠-觉醒周期的影响5.1大鼠DRN内微量注射L-Glu(0.2μg),同时在双侧RT内微量注射NS (1.0μg)后,与对照组(DRN和双侧RT注射NS, 0.2μg)比较,睡眠时间减少,觉醒时间增多;大鼠DRN内微量注射L-Glu(0.2μg),同时在双侧RT内微量注射二甲基麦角新碱(methysergide, MS, 1.0μg )后,与对照组(DRN注射L-Glu 0.2μg,双侧RT注射NS 1.0μg)比较,睡眠时间增多,觉醒时间减少。5.2大鼠DRN内微量注射对氯苯丙氨酸(p-chlorophenylalanine,PCPA,10μg),同时在双侧RT内微量注射NS (1.0μg)后,与对照组(DRN和双侧RT注射NS, 1.0μg)比较,睡眠时间增多,觉醒时间减少;大鼠DRN内微量注射PCPA(10μg),产生睡眠增多效应后,在双侧RT内微量注射5-羟色胺酸(5-hydroxytryptaphan , 5-HTP, 1.0μg )后,与对照组(DRN注射PCPA 10μg,双侧RT注射NS 1.0μg)比较,睡眠时间减少,觉醒时间增多。
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The results indicated that the strength of corner joints increased with the increase, of thickness, density, moisture content, MOR and IB of the board, and that the measure of coefficient of variability for MOR and IB of the board would play a more important role in the stability and strength of corner Structure in furniture.
本试验中,以木质圆榫不可拆连接角结构抗变形强度和偏心凸轮连接角结构抗破坏变形强度为最高,试验的综合结果表明,可拆型连接的角结构强度总是低于不可拆型连接的角结构;角结构承受拉载的能力明显低于承受压载的能力。
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Three optimal structures were gotten in this paper by the model of elastic modulus along the grain, elastic modulus in tangential direction, in-plane shearing modulus and laminate rigid:①0/90/0/90/0/90/0/90/0, the outside four veneer is modified, the along grain MOE and MOR are 8377MPa, 60.9MPa respectively; The across grain MOR is 35.1 MPa, and TS is 17.2%.
4通过应用杨木单板顺纹弹性模量、横纹弹性模量、面内剪切模量及层合板刚度模型进行杉木胶合板结构设计,经多次修正,得出较好结构有以下几组:①0/90/0/90/0/90/0/90/0,最外四层为杉木改性单板,顺纹弹性模量和静曲强度分别达到了8377MPa,60.9MPa;横纹静曲强度为35.1 MPa,吸水厚度膨胀率为17.2%。
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Group II (N0.015M) received NEO 0. 015mg/kg with MOR 0.025 mg/kg caudally. Group III(N0.02M) received NEO 0.02 mg/kg with MOR 0.025 mg/kg caudally.
按随机双盲法将患儿分成3组,每组15例:Ⅰ组,MOR 0.025mg/kg;Ⅱ组N_(0.015M组,NEO 0.015mg/kg+MOR 0.025mg/kg;Ⅲ组N_(0.02M组,NEO 0.02mg/kg+MOR 0.025mg/kg。
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The diffusivity of ethanethiol in flexible MOR is higher than that in flexible MFI, because the 12 member ring in MOR is larger than the 10 member ring in MFI.
对比这两种沸石,MOR中的扩散系数要比MFI沸石中大,这是因为乙硫醇分子在MOR12元环中扩散更易进行所致。
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The topology models of MFIand MOR were built and the method of Configurational-bias Monte Carlosimulation was used for investigating the adsorption and distributions of ethanethiolas adsorbate in MFI and MOR type zeolites. The diffusion of ethanethiol in MFI andMOR type zeolites was studied by molecular dynamics simulation.The results show, the concentration of Zn2+ is the most important factor of theloading quantity of zeolite 13X and alumina, and the temperature secondly. The pHvalue which range from 2 to 3 effects on the loading quantity faintly.
构建了MFI 和 MOR 两种拓扑结构沸石的数学模型,以乙硫醇分子作为被吸附的目标物,采用偏倚蒙特卡罗方法模拟乙硫醇在MFI和MOR沸石上的吸附行为及分子分布密度,并利用分子动力学模拟乙硫醇在这两种拓扑结构的扩散行为,为实现面向硫化物进行吸附剂的微观结构设计作初步探索。
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The relation to the incentive effect of incentive price controlling strategy with the area by the extending of surplus function and abscissa axis surrounding is discussed, the criteria of incentive effect are given, at the same time the definition of the incentive strategy and the judging theorem of one is mor...
讨论了激励价控策略的激励效应与以用户盈余函数的扩展函数为曲边的曲边梯形面积的关系,给出了激励价控策略对用户激励效应的性能指标,以及一个激励策略比另一个激励策略更有效的定义及其判断定理。
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Mechanical properties including the density MOR, MOE, shearing strength of birch and basswood LVL beams made by the bi-frequency hot press were tested by universal strength testing machine. The primary failure modes were discussed when LVL beams suffered the flexural and level shearing load.
在万能力学试验机上测试由高频热压方法制造的桦木和椴木单板层积梁的密度、静曲强度、弹性模量和水平剪切强度等力学性质;讨论LVL梁受弯曲、水平剪切载荷时的主要破坏形式并得出相关结论。
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Compared with the warlords battle royal and the change of regime in the period of north-ocean government, mor
文章以前五章为基本史实描述,支持最后一章的历史评价,构成一个大因果关系。但在前五章的事实描述,也有一定的结果评价,分别?
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Further analysis of the results showed those who were brainiest as children were mor e likely to have become vegetarian as adults.
研究人员通过进一步分析发现,智商较高的儿童成人后成为素食者的可能性较大。
- 相关中文对照歌词
- Metamorphosis
- Es Amor
- Rugadh Mi 'teis Meadhan Na Mara (Me Zo Ganet É Kreiz Er Mor/I Was Born In The Midst Of The Sea)
- 1984
- Sí Do Mhaimeó Í
- Mae Hiraeth Yn Y Mor - Moderato
- Aniron (Theme For Aragorn And Arwen)
- S' Fagaim Mo Bhaile
- Òran Nan Raiders
- All Through The Night (Welsh Version)
- 推荐网络例句
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Consumer preference ; fuzzy partial ordering relation ; condorect function
消费者偏好;模糊偏序关系; condorect函数
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And she doesn't have a cat, she has a Mexican hairless.
而且她不养猫,她养了一只墨西哥无毛犬。
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This is the time to push rather than to slacken our efforts.
今后更应向前推进一步,决不能松懈我们的努力。