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The following methods were used in the study, such as viral inoculation of animal, pathological method, flow cytometry, RT-PCR, in situ hybridization, immunohistochemistry and terminal deoxy nucleotidyl transferase mediated dUTP nick end labling . Morphological changes of the infected chickling, dynamic changes of T, B cells and T subsets, changes of adhesion molecules on the lymphocyte surface, necrosis and apoptosis of lymphocyte and neurons, changes of MIP-1β and IL-8 producing cells in the brain, cell types of perivascular tuffing in cerebral tissue were systematically studied.

在研究过程中,采用了病毒接种技术、普通病理学研究方法、流式细胞仪技术、RT-PCR技术、原位杂交技术、免疫组化技术、凋亡细胞末端标记技术,系统研究了不同日龄的SPF雏鸡人工感染AEV-NH937株后的病理变化,T和B淋巴细胞、T淋巴细胞亚群的动态变化规律,淋巴细胞表面某些粘附分子的变化,淋巴细胞和神经元的坏死与凋亡,雏鸡脑组织中产生趋化因子MIP-1β和IL-8细胞的变化规律,脑组织中围官性细胞浸润的细胞类型。

The following methods were used in the study, such as viral inoculation of animal, pathological method, flow cytometry, RT-PCR, in situ hybridization, immunohistochemistry and terminal deoxy nucleotidyl transferase mediated dUTP nick end labling. Morphological changes of the infected chickling, dynamic changes of T, B cells and T subsets, changes of adhesion molecules on the lymphocyte surface, necrosis and apoptosis of lymphocyte and neurons, changes of MIP-1β and IL-8 producing cells in the brain, cell types of perivascular tuffing in cerebral tissue were systematically studied. The key research results were.(1)The average percentage of CD19+cell in blood, bursa and spleen, after 3, 5 days of inoculation, was significantly Phybridization revealed that the number of IL-8 and MIP-1βproducing cells was increased in the infected brain.

在研究过程中,采用了病毒接种技术、普通病理学研究方法、流式细胞仪技术、RT-PCR技术、原位杂交技术、免疫组化技术、凋亡细胞末端标记技术,系统研究了不同日龄的SPF雏鸡人工感染AEV-NH937株后的病理变化,T和B淋巴细胞、T淋巴细胞亚群的动态变化规律,淋巴细胞表面某些粘附分子的变化,淋巴细胞和神经元的坏死与凋亡,雏鸡脑组织中产生趋化因子MIP-1β和IL-8细胞的变化规律,脑组织中围官性细胞浸润的细胞类型。

It is postulated that IP induces the release of endogenous protective molecules that interact with cognate receptors to activate PLC through mediation of the chincough virus sensitive proteins. PLC hydrolyzes membrane phospholipid and produces diacylglyccerol,that,by working together with calcium hydronium,translocates PKC from cytoplasm to cell membrane and activates PKC.

推测缺血预处理细胞保护作用机制是:缺血预处理引起内源性保护物质释放,作用于细胞膜上的相应受体,通过百日咳病毒敏感性G蛋白的介导而激活磷脂酶,磷脂酶水解膜磷脂,产生二酰基甘油(diacylglyccerol,DAG),DAG和Ca2+协同作用于PKC,使其从胞浆转位到细胞膜并且被活化,PKC在膜上使底物蛋白磷酸化,引发一系列相关信号转导通路的激活,调控保护性基因的表达,从而提高细胞对缺血再灌注损伤的耐受性。

The destruction of ozone molecules in the ozone layer by chemical reaction with materials released from human activities, including chlorofluorocarbons and halons.

从人类活动中释放出来的物质(包括氟氯烃和卤代烷哈龙)同臭氧层中的臭氧分子发生化学变化,导致其破坏。

The valence electron structure of medium chrome cast iron matrix is set up and the effect of Si on medium-temperature phase transformation is analyzed by using empirical electron theory of solids and molecules.

此文建立了290Cr8Si2中铬铸铁基体的价电子结构,运用固体与分子经验电子理论分析了中铬铸铁中Si对铸铁中温相变的影响,分析和实验结果表明,290Cr8Si2中铬铸铁基体含C、Cr、Si的-γFe晶胞中,C原子与Si原子的结合力强于C原子与Cr原子的结合力,中铬铸铁中较高的含Si量降低了铸铁基体的含Cr量,进而降低了中铬铸铁的淬透性。

There is only one Schiff base chromophore in its molecules and it has less innermolecular strains.

由以上分析可见,化合物的分子结构对化合物的光致变色性质存在着影响。

In feed add glucanase, xylanase, cellulose-based compound enzyme enzymes, anti-nutritional factors can degrade into small molecules fragments, the release of nutrients, reduce chyme viscosity, so that nutrition the material can be fully digested and absorbed to reduce the ileum bacteria substrate, inhibiting growth and reproduction of harmful micro-organisms to prevent diarrhea.

在饲料中添加以-葡聚糖酶、木聚糖酶、纤维素酶等为主的复合酶制剂,能够将抗营养因子降解成小分子片段,释放营养物质,降低食糜粘度,使营养物质能被充分消化吸收,减少回肠中细菌的底物,抑制有害微生物生长繁殖,防止腹泻。

MSODa can inhibit the expression of the adhesion molecules、 CINC/IL-8、NO and free radicals, consequently skeletal muscle ischemia/reperfusion injury is prevented.

MSODa减少氧自由基的生成,通过降低CINC/IL-8、NO,抑制粘附分子的表达,而减轻骨骼肌I/R损伤,且较SOD作用更好而持久。

The proper in-situ polymerization systems of acidic and basic compounds using amino acid derivatives and cinchona alkaloids as template molecules respectively were adopted for preparation of molecularly imprinted monolithic columns by systematically investigating the effect of polymerization conditions on pore structure and selectivity of resulting polymers. The enantiomers of amino acid derivatives and the diastereomers of cinchona alkaloids were successfully resolved on these columns.

本文分别以氨基酸衍生物和辛可纳生物碱为模板分子,系统研究了制备条件对聚合物孔结构和选择性的影响,建立了合适的酸性化合物和碱性化合物的原位印迹体系。

Vibrational circular dichroism is a useful technology to measure the circular dichroism of chiral molecules in the IR region, which remarkably expands the application of circular dichroism to the determination of AC.

振动圆二色谱是在红外波长区域测定分子圆二色性的一种新方法,极大地扩展了圆二色谱的应用范围。

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