查询词典 mitochondria

The shape of chloroplast didn't change after seedling was treated by GA〓, but the number of lamllasomes increased, and starch grain enhanced. These changes were accord to that of the rate of photosynthesis. Mitochondria varied greatly, the structure of mitochondria could see vaguely and carinace almost deliquesce after 4days. Enrolucrum emerged until the seventh day after treatment and endomembrance of mitochondria recovered gradually, cristae could be seen. On the other hand, the endomembrance of mitochondria in CK (normal non-bolting) was obvious and the quantity of cristae in the mitochondria was much more than the GA〓 treatment. This showed that respiration might decrease after GA〓 treatment while reduced the depletion of photosynthesis products and enhanced bolting.

GA〓处理后抽苔过程中叶绿体形状未发生变化，但类囊体增多，淀粉粒增多，这种变化与光合速率的变化吻合；线粒体变化较大，处理后4天线粒体结构较模糊，嵴不清晰，7天时外膜形成，内膜也逐渐恢复，能见到嵴，而对照线粒体内膜结构明显，嵴清晰数量也多，说明GA〓处理后，呼吸作用降低，减少光和产物的消耗；细胞膜无破损，较光滑完整，说明对细胞膜没有伤害。

In addition, we studied the distribution of mitochondria in metaphase Ⅱ rabbit oocytes, zygotes and early embryos by two active mitochondria dyes and revealed the change of active mitochondria during early development of rabbit embryos. We also found that the distribution of microfilaments and microtubules have similar patterns to that of active mitochondria, which is an important basis for further development of animal cloning study using the recipient oocytes derived from rabbit.

此外，我们应用两种线粒体荧光染料研究了日本大耳白兔体内成熟卵母细胞、正常交配后收集的受精卵及早期体外发育胚胎的活性线粒体的位置变化，首次揭示了兔早期胚胎中活性线粒体的变化特征及其与微丝微管变化的相关性，为进一步开展以兔卵母细胞作为核移植受体的动物克隆研究打下了重要的基础。

The invention discloses a method to analyze fish genetic information with mitochondria DNA D-loop controlling zone, which comprises the following steps:(1) extracting genom DNA and mitochondria DNA of fish;(2) designing simple primer in fish mitochondria DNA D-loop controlling zone; possessing twenty one basic groups CAC CCY TRR CTC CCA AAG CYA in upstream primer MitD1-F; possessing twenty three basic groups GGT GCG GRK ACT TGC ATG TRT AA in downstream primer MitD1-R;(3) proceeding PCR reaction under the function of primer;(4) choosing agarose jel electrophoresis;(5) checking sequence for augment mtDNA D-loop gene fragment.

本发明公开了一种利用线粒体DNA D-loop控制区进行鱼类遗传信息分析的方法。它包括如下步骤：(1)分别提取鱼类基因组DNA和线粒体DNA；(2)在鱼类线粒体DNA D-loop控制区设计简并引物：其上游引物MitD1-F有21个碱基：CAC CCY TRR CTC CCA AAG CYA；下游引物MitD1-R有23个碱基：GGT GCG GRK ACT TGC ATG TRT AA；(3)在引物的作用下进行PCR反应；(4)琼脂糖凝胶电泳；(5)对扩增的mtDNA D-loop基因片断进行测序。

The following five reviews published in this volume summarize recent discoveries in this area: DNA Replication and Transcription in Mammalian Mitochondria, Mitochondrial-Nuclear Communications, Translocation of Proteins into Mitochondria, The Machines that Divide and Fuse Mitochondria, and Why Do We Still Have a Maternally Inherited Mitochondrial DNA?

这一卷发表的以下五篇综述总结了这个领域的最新发现：哺乳动物线粒体中的DNA复制和转录，线粒体-细胞核通信，蛋白质到线粒体的迁移，分离和融合线粒体的机制及为什么我们仍然有一个母系遗传的线粒体DNA？

RESULT: 1.Ouabain act on lens sodium-pump,under the LM,lens anterior capsular membrane discontinuous,epithelium cells clustered,occluding zonule seperated,lens fiber layers fractured.Under the EM,cells totally hollowed,mitochondria swelling,myelin figure appeared.RT-PCR examine the expression condition of αsubunit of sodium pump on mRNA level,α1、α2 and α3-isoform are all decreased.2.Digoxin act on lens sodium-pump,under the LM,lens cell oedema,linkage distructed,extensive exfoliation.Under the EM,plasma appeared little half-transparant hollow region,mitochondria swelling and ridge disappeared. RT-PCR examine,α1、α2 and α3-isoform are all decreased.3.Amphotericin B act on lens sodium-pump,under the LM,lens epithelium cells linked tightly,arranged in-line,lens fiber layers arranged tightly and regularily.Under the EM,abbundant cellular organes,exuberant cells function indicated. RT-PCR examine the expression condition of αsubunit of sodium pump on mRNA level,α1 and α3-isoform are increased significantly,demonstrated isoform-specific action.4D-thyroxine act on lens sodium-pump,under the LM,lens plasmalemma integrated,cells arranged tightly and regularily.Under the EM,nucleus fission appeared,desmosome half-desmosome and tensile microfilaments linked the cells. RT-PCR examine,α2 and α3-isoform are increased, also demonstrated isoform-specific action.5.Vitamin E act on lens sodium-pump,under the LM,lens anterior capsular membrane continuous and smooth,epithelium cells tightly linked,lens fiber layers appearede hollow region occasionally.Under the EM,lateral membrane high density belt appeared,abundant nucleolus. RT-PCR examine,onlyα1-isoform are increased, demonstrated significantly isoform-specific action.6.DMSO act on lens sodium-pump,under the LM,lens anterior capsular membrane slightly thicker,cells linkage partly distructed.Under the EM,plasmalemma denaturation,mitochondria swelling.RT-PCR examine,α1、α2 and α3-isoform are all altered slightly and haven't significant meanning.

结果：1、哇巴因作用于晶状体钠泵后，光镜下晶状体前囊膜断裂、上皮细胞聚积、闭合连接分离、纤维板层裂隙，电镜下全层细胞空泡化、线粒体肿胀出现髓样结构，RT-PCR法检测晶状体钠泵α亚单位α1、α2及α3三种重整异构体在mRNA水平的表达均减弱。2、地高辛作用于晶状体钠泵后，光镜下晶状体细胞水肿、细胞连接破坏、广泛剥离，电镜下胞质见少许半透明空化区、线粒体肿胀嵴消失，RT-PCR法检测晶状体钠泵α亚单位α1、α2及α3三种重整异构体在mRNA水平的表达均减弱。3、两性霉素B作用于晶状体钠泵后，光镜下晶状体上皮细胞紧密连接、线状排列、纤维板层紧密规整，电镜下细胞器丰富、细胞功能旺盛，RT-PCR法检测α1及α3表达显著增强、具有一定的重整异构作用特异性。4、D甲状腺素作用于晶状体钠泵后，光镜下晶状体质膜完整、细胞排列紧密规整，电镜下胞核见分裂像、细胞间有桥粒、半桥粒及张力微丝，RT-PCR法检测α2及α3表达均增强、亦有一定的重整异构作用特异性。5、维生素E作用于晶状体钠泵后，光镜下晶状体前囊膜连续光滑、上皮细胞紧密连接、纤维板层偶见空化，电镜下囊侧膜内有高电子密度带、细胞核仁丰富，RT-PCR法检测仅有α1的表达显著增强、具有极强的重整异构作用特异性。6、二甲基亚砜作用于晶状体钠泵后，光镜下晶状体前囊膜轻度增厚、细胞连接部分破坏，电镜下质膜变性、线粒体肿胀，RT-PCR法检测晶状体钠泵α亚单位α1、α2及α3三种重整异构体在mRNA水平的表达无显著改变。

When the soil relative water content is 80%, the chloroplast and mitochondria cristae were regular. When the soil relative water content is 100%and 60%, the chloroplasts became roud in shape.There were bigger giqantic starch grains and fat droplets in the chloroplasts. Mitochondria cristae became swollen. When the soil relative water content is 40%and 20%, thylakoids became swollen and disorder in leaf cell, mitochondria cristae became obscured .

5在土壤相对含水量为80%时，叶绿体和线粒体能维持其结构和膜的完整性；在土壤含水量为100%和60%时，毛桃和石头扁桃叶片中的叶绿体变圆，出现大颗粒淀粉和脂类小滴，线粒体嵴膨胀；在土壤含水量为40%和20%时，叶绿体类囊体紊乱肿胀，线粒体嵴模糊或溶解。

Results showed that there were the morphological changes of mitochondria, such as vacuolization, degeneration and cristae swelling or disappearance, in HT-29 cells treated by C2-ceramide for 24 hours. Under the fluorescence microscope, mitochondria of apoptotic cells aggregated and emitted red fluorescence, however, mitochondria of normal cells emitted green fluorescence. The mitochondrial membrane potential began to decrease after 6 hours in HT-29 cells treated with C2-ceramide in time and dose dependent manner.

结果显示C2-神经酰胺作用HT-29细胞24小时，线粒体发生空泡化、变性、嵴肿胀或消失等形态学改变；此时荧光显微镜下可观察到线粒体聚集，发出红色荧光，而正常细胞线粒体发出绿色荧光。C2-神经酰胺作用细胞6小时，线粒体膜电位即开始降低，呈时间-剂量-效应关系。

In addition, spermiogenesis includes chromatin condensation, migration of the cytoplasm and mitochondria to the caudal pole of the nucleus, formation of a moderate axial nuclear fossa containing the proximal centriole and part of the distal centriole and presence of a short cytoplasmic canal separating mitochondria from the flagellum.

另外，精子发生过程中还包括染色质浓缩，细胞质和线粒体向细胞核的尾端迁移，在核的后端中轴位置上形成中等大小的核后凹，近端中心粒和远端中心粒的一部分嵌在核后凹之内，短的胞质内陷管将线粒体与鞭毛分隔开。

Changes of lung ultrastructures: The results of electron microscope showed that in group 1, there were swelling mitochondria was arranged in disorder, less matrix, and hyalomere appeared, thin double-deck membrane in 50% samples: Interalveolar septum stroma was with edema in 60% samples; Polymorphonuclear leukocyte gathered in vessel or emigrated out of vessel in 50% samples; In group 2, double-deck membranes of mitochondria were integral, densely matrix showed micro-granule shape in 90% samples; Pinocytosis in epithelial cells of type Ⅰ lung strengthened, being destroyed, and pinocytosis in endothelial cells strengthened in 10% samples; lnteralveolar septum stroma was with edema, no polymorphonuclear leukocyte gathered in vessel or emigrated out of vessel or corpuscule was empty in type Ⅱ alveolar epithelium in 20% samples.

肺组织超微结构改变观察：纯氧机械通气组50%标本线粒体肿胀、排列紊乱，基质变浅，出现透明区，双层膜变薄，60%标本肺泡隔间质水肿，50%标本多形核白细胞血管内聚集或游出血管外现象；34%氧浓度机械通气组90%线粒体双层膜较完整，基质致密呈细颗粒状；10%标本可观察到Ⅰ型肺上皮细胞有吞饮增强、破坏及内皮细胞吞饮增强；20%标本肺泡隔间质水肿；未见到多形核白细胞血管内聚集或游出血管外现象及Ⅱ型肺泡上皮板层小体排空。

Al stress badly destroyed the ultra-structure of root tip cells, the nucleus changed uneven, the numbers of vacuole and mitochondria increased but crista of mitochondria changed unclear and almost disappeared.

与对照相比，最明显的变化是细胞核结构变得不完整，同时空泡增多，线粒体数量增加，但线粒体脊比较模糊甚至消失。4。

But GST= 0.156, Nm=1. 588. As a result, the foundation of Youyongchi Avicennia marina population was the result of the migration of hypocotyles and human factors.

这项工作可以为海岸防护林中新引进种类的判定以及为研究种群建立者效应方法的确定提供科学依据。

The two-dimensional CDRC-ADI-FDTD update equations for collision unmagnetized plasma are induced. The unconditional stability of the CDRC-ADI-FDTD formulation for collision unmagnetized plasma is obtained by the examples.

推导了碰撞非磁化等离子体中的二维CDRC-ADI-FDTD迭代公式，并用算例验证了碰撞非磁化等离子体CDRC-ADI-FDTD算法也是无条件稳定的。