查询词典 medium of circulation

The aim of thesis is take analysis of medium morphosis, factors of medium morphosis and arguments of the influence of medium morphosis to advertisement morphosis, tell the performance of new and old medium advertisement influence of variety trend at new medium technique application descend, then announce to the diversification development trend of advertisement morphosis, and provide suggestions for advertisement medium choice.

本论文旨在通过对媒介形态变化的梳理、对媒介形态变化影响因素的分析及对媒介形态变化如何影响广告形态变化的论证，推演出在新媒体技术应用下新老媒体广告影响力的变化趋势，进而揭示广告形态的多元化发展趋势，并为广告行业的媒介选择策略提供建议。

First is enhances the boiler the heat energy transfer rate,(1) changes the vertical chimney to the horizontal-type chimney, reduces the speed of flow, Increase the time which the actuating medium and the boiler hot flame doing heat change;(2) changes the high temperature system sole medium sole heat transfer area to many kinds of medium the multi-channel heat transfer area(2-6 medium, 2-6 heat transfer area, this article takes four medium, 4 heat transfer areas confer elaboration), the increase heat transfer flow path through many times heat absorption,Lets the pot furnace coal flame heat energy as far as possible much transmit gives the actuating medium, Enables the quantity transfer rate from the present 60%--75% enhances to is bigger than 95

第一是提高锅炉的热能转移率，（1）将立式烟囱改为卧式烟囱，降低流速，增加工作介质与锅炉热焰气的热交换时间；（2）将高温系统单一介质的单一换热区改为多种介质的多道换热区（2—6种介质， 2—6道换热区，本文取四种介质，4道换热区加予论述），增加换热流程，通过多次吸热，让工作介质尽可能多地把锅炉煤焰气的热能传递给工作介质，使能量转移率从现在的60%--75%提高到大于95

A human promyelocytic leukemia cell mutant (HL-60-AR) with deficiency in HGPRT and resistance to 8-AG was established by treat〓nt of the HL-60 cells with MNNG and prescreened in 0.3% agar RPMI-1640 semisolid medium containing 8-AG. The mutant cells show the following characteristics:(1) resistant to cytotoxic action of 8-AG and growing vigorously in medium containing 20ug/ml 8-AG;(2) capable of forming 〓〓 colonies in soft agar medium in the presenee of 8-AG:(3) highly sensitive to HAT selective medium and fail to growing in HAT medium of form colonies in soft agar medium containing HAT;(4) completely deficinet in HGPRT activity; and (5) no radioactive silver grains over the mutant cells due to the absence of -hypoxanthine incorporation showed by radioautography.

一、用MNNG诱变和8-AG进行抗性筛选，成功地建立了一株抗高浓度8-AG和完全缺失HGPRT活性的人早幼粒白血病细胞突变株(HL-60-AR)，该细胞能抵抗8-AG的毒性作用，对HAT选择培养基十分敏感，检测不到HGHRT活性，不能参入〓H一次黄嘌呤。

The BMSCs were divided into six groups after repeatedly passaged: A,the BMSCs were cultured with conventional culture fluid(DMEM culture fluid+20%fetal bovine serum+2 mmol/L aminoglutaric acid amine) all the time;B,the BMSCs were cultured with conventional culture fluid+HGF(25ng/ml)+dexamethasone10~(-7M;C(HGF and Zuoguiwan induced group), the BMSCs were cultured with conventional culture fluid+ HGF(25ng/ml)+ dexamethasone10~(-7M+ 10%Zuoguiwan drug serum;D(conditioned medium and contrast serum induced group), the BMSCs were cultured with conventional culture fluid+50 % conditioned medium+10 % normal rat serum;E(conditioned medium and Bazhentang drug serum induced group), the BMSCs were cultured with conventional culture fluid+50 % conditioned medium+10 % Bazhentang drug serum;F(conditioned medium and Zuoguiwan drug serum induced group), the BMSCs were cultured with conventional culture fluid+50 % conditioned medium+10% Zuoguiwan drug serum.

常规培养组始终使用常规培养液(DMEM培养液+体积分数20％胎牛血清+2mmol／L谷氨酸胺)进行培养；HGF诱导组以常规培养液+促肝细胞生长因子(HGF，25ng／ml)和地塞米松10~(-7M进行培养；HGF加左归丸组以常规培养液+促肝细胞生长因子(HGF，25ng／ml)和地塞米松10~(-7／M+10％的左归丸含药血清进行培养；条件培养液加对照血清组以常规培养液+50％的条件培养液+10％正常大鼠血清进行培养；条件培养液加八珍汤组以常规培养液+50％的条件培养液+10％八珍汤含药血清进行培养；条件培养液加左归丸组以常规培养液+50％的条件培养液+10％左归丸含药血清进行培养。

The hFCECs were cultured by sticking tissues piece method that the cornea were divided into the limbus and central tissue piece and digestive method,respectively.For digestive method,the digestive juice of 5mg/ml DispaseⅡ+0.25%trypsin was chosed.D/F12 with 10%fetal bovine serum and 100 U/ml penicillin and streptomycin were used in this study,the culture condition was 37℃and 5%CO2,and culture medium changed every three days2、Passage of hFCECsAfter more than 80%confluenced,cells from corneal limbus hFCECs were digested for 5-15min with different concentrations of trypsin/EDTA at 37℃,and then passaged at a ratio of 1:2.The cells were subcultured on empty plates,mouse 3T3 fibroblast feeder layer,fetal corneal stromal cell feeder l ayer or HTK feeder layer respectively.And D/F12, mouse 3T3 fibroblast conditioned medium,fetal corneal stromal cells conditioned medium or HTK conditioned medium with 10%FBS and 100 U/ml penicillin and streptomycin were used respectively as the culture medium.And the culture medium were changed every three days as well.

方法一、人胎儿角膜上皮原代和传代培养1、原代培养严格无菌操作获取人胎儿角膜片，采用组织块贴壁法、酶消化法原代培养人胎儿角膜上皮细胞。2、传代培养角膜缘部的细胞生长融合达80%以上，不同稀释浓度的胰酶/EDTA消化液消化传代分别接种于空板、小鼠3T3成纤维细胞饲养层、胎儿角膜基质细胞饲养层及HTK饲养层，培养液分别采用D/F12、小鼠3T3成纤维细胞条件培养液、胎儿角膜基质细胞条件培养液、HTK条件培养液。

We shall change functions, regard the support to the development of small and medium circulation enterprises as functions of competent commercial departments, and focus on such support, fortify the sense in serving small and medium circulation enterprises, study specific measures to solve difficulties and problems encountered by these enterprises on their way to development, reinforce media and publicity work, fully utilize the news media to create a sound environment of public opinions for the development of such enterprises.

要转变职能，结合地方机构改革，将扶持中小流通企业发展作为商务主管部门的职责和重点工作。要牢固树立为中小流通企业服务的意识，针对他们在发展中遇到的困难和问题，研究具体措施予以解决。要加强舆论宣传，充分利用新闻媒体，营造中小企业发展的良好舆论氛围。

We shall actively advocate the application of modern management techniques of science and technology nature, raise capabilities of small and medium circulation enterprises of applying modern scientific and technological achievements, support these enterprises to transform technologies, change their images, play an active part in adopting modern means including the computer management, promote the use of point sales system in the operations of retail enterprises and their stores, gradually establish and improve the Management Information System, energetically create conditions and enhance the information level of small and medium circulation enterprises.

积极倡导采用现代化科技管理技术，提高中小流通企业运用现代科技成果的能力。支持中小流通企业开展技术改造，改变店容店貌，积极应用计算机管理等现代化手段，在零售企业和门店经营中推广使用时点销售系统，逐步建立和完善信息管理系统，积极创造条件，提高中小流通企业的信息化水平。

We shall drive small and medium circulation enterprises to implement brand strategies, vigorously develop characteristic, specialized and branded operations, foster a batch of famous brands, carry forward and develop China's time-honored brands with great efforts; shall offer guidance to the said enterprises to carry out disturbed operation and change the status of old and identical business modes, lack of service brand and inferior methods of competition focusing on "price war"; shall actively promote cooperation, franchise operation, voluntary chains and other methods to set up a number of small and medium circulation enterprises with famous service brands and advantages of subjects of diversified investment, with support from competitive enterprises.

推动中小流通企业实施品牌战略。大力发展特色化、专业化、品牌化经营，培育一批中小企业的知名品牌，弘扬和发展中华老字号。引导企业实行错位经营，转变经营方式上陈旧雷同、缺乏服务品牌和以"价格战"等低层次竞争手段为主的状况。积极倡导通过联合、合作、特许经营和自由连锁等方式，依托有竞争力的企业，形成一批拥有著名服务品牌、多元投资主体的优势中小流通企业。

"When the galvanizing line is to be put in operation, the circulation pump is started to feed medium from circulation tank into rinsing tank and then the medium is sprayed on the strip ."

镀锌生产时，循环泵把介质从循环槽泵入喷淋槽，介质就喷在钢带上。

The results indicated:0.1 mg/L 2,4-D or 0.1 mg/L 2,4-D+0.2 mg/L KT was optimal to induct the callus of carrot hypocotyls.The majority of the callus induced on the medium containing 0.1 mg/L 2,4-D regenerated embryoid,whereas,the callus induced on the medium containing 0.1 mg/L (2,4-D)+0.2 mg/L KT produced adventive buds.During the growth of regenerated plant,MS medium is available for rooting of seedling,and seedling regenerated on B5 medium must be placed on the B5 medium containing 0.1% IBA to develop root.

试验结果表明：0 1mg/L2，4 D或0 1mg/L2，4 D+0 2mg/LKT的激素组合有利于胡萝卜下胚轴的愈伤组织诱导；在0 1mg/L2，4 D的培养基上形成的愈伤组织主要以胚状体的形式再生，而在含0 1mg/L2，4 D+0 2mg/LKT激素组合的培养基上形成的愈伤组织主要以发生不定芽的方式再生；再生过程中，B5基本培养基上的苗子不易生根，需要附加0 1mg/L的IBA，而在MS培养基上苗子可直接形成根。

Based on the construction of international financial centre, we have pushed forward the explorations in the integration of cultural industries with financial services.

2010年及整个"十二五"期间，上海文化产业面临更有利的发展时机、更坚实的发展基础和更完善的发展环境。

The koala is an animal with a large bag in its body.

树袋熊用它的身体的一个大的袋子是一只动物。