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In the two-site sandwich CLIA for TSH,two anti-TSH monoclonal antibodies were involved,one was coated on microwells and another was labeled with acridinium ester. The assay sensitivity is 0.01mlU/L. Intrassay and interassay coefficients of variation are 4.29-6.71 and 4.36-6.14%,respectively. Average recovery is 98%.

以 DMAE·NHS为发光标记物，研究建立了两种 TSH CLIA方法，均采用一步夹心免疫分析法，在第一种方法中一株抗TSH单克隆抗体包被微孔板，另一株标记DMAE·N'HS，方法的分析灵敏度为0.olmIUA，批内变异系数为 4.29巧。71％，批间变异系数为 4.36ed.14％，平均回收率为 98％，与 TSH IRMA法的相关方程为 Y－0.20＋0.92X，相关系数为 0.993，与 Ciba Co讪ng TSH CLIA测量值的相关方程为 Y—0.16＋0.92兄相关系数为 0.986。

Chemiluminescent immunoassay is one of the advanced immunoassay ofnon-radioisotopic immunoassay because of its high sensitivity, wide dynamic range,high accuracy, stable labeled protein, full automation and extensive application field.CLIA using acridinium ester derivatives as chemiluminescent label has advantages oflow background, high signal-to-noise ratio, no need of catalyst and simpleluminescence system. Different aspects associating the DMAE·NHS-based CLIA were studied in thisthesis, including synthesis of acridinium ester, chemiluminescentcharacteristics of DMAE·NHS, labelling antibody or streptavidin with DMAE·NHS,two-site sandwich chemiluminescent immunoassay for TSH, two-sitesandwich chemiluminescent immunoassay involved biotin-streptavidinsystem for TSH, and competitive chemiluminescent immunoassay usingbiotin-streptavidin system for TT〓. The desired acridinium ester, DMAE·NHS, was synthesized according to thereference method with some modifications. The products were identified by IR, NMR,MS and elemental analysis. In our method, KOH was used in place of NaOH tosynthesize benzyl ester of 3,5-dimethyl-4-hydroxybenzoic acid,2',6'-dimethyl-4'-(n-succinimidyloxycarbonyl) phenyl-acridinium-9-carboxylate waspurified on a silica gel column with chloroform/ethylacetate(4:1, v/v) as eluent andfurther purified by triturating with hexane/acetone(2:1, v/v).

DMAE·NHS的合成是本论文的关键和难点，我们对文献方法进行改进：文献方法用氢氧化钠与3，5-二甲基-4-羟基苯甲酸反应制得3，5-二甲基-4-羟基苯甲酸钠，再用3，5-二甲基-4-羟基苯甲酸钠与苄氯作用制备3，5-二甲基-4-羟基苯甲酸苄酯，我们用氢氧化钾代替氢氧化钠，使合成取得成功；在合成2'，6'-二甲基-4'-苯基-吖啶-9-甲酸酯时，文献方法对粗产品进行两次硅胶柱层析纯化，第一次柱层析用氯仿／乙酸乙酯(4∶1，V∶V)作溶剂和淋洗剂，第二次柱层析用己烷／丙酮(2∶1，V∶V)作溶剂和淋洗剂，按照文献方法得到的不是所需要的化合物，我们只进行第一次柱层析纯化，然后用己烷／丙酮(2∶1，V∶V)进行研磨，过滤，洗涤，除去溶于己烷／丙酮(2∶1，V∶V)的部分，得到所需要的产品。

For better understanding of the formation and changes of glycoconjugates during spermatogenesis and oogenesis, the distribution of PNA binding glycoconjugates in the plasma membrane of Adda cinera germ cells have been detected by using biotin labeled PNA.

本文首次采用生物素标记的花生凝集素对中华蚱蜢配子发生过程中细胞质膜的糖蛋白含有糖基Gal-β-(1，3-GalNAC进行了标记，旨在认识配子发生过程中细胞质膜糖复合物的形成与变化规律，探讨其所具有的功能。

AIM: To study the method of tracking of allogenically grafted rat bone marrow stem cells labeled with Feridex in rat liver.

目的：研究菲立磁标记的大鼠骨髓基质干细胞在同种异体移植鼠肝脏中的追踪方法。

AIM: To study the method of tracking of allogenically grafted rat bone marrow stem cells labeled with Feridex in rat liver. METHODS:Feridex was used to label isolated BMSCs.

目的：研究菲立磁标记的大鼠骨髓基质干细胞在同种异体移植鼠肝脏中的追踪方法。

The detectable group, biotin-ε-aminocaproic acid N-hydroxysuccinimide ester, is attached to the remaining amino group. A series of multiamine compouds, i. e. ethylendiamine, 1, 6-hexandediamine, triethylenetetramine, tetraethylenepentamine, and synthetic polyarylamide are used inlabelling, it is found that linker arms and steric hindrance effect the detection sensitivity. The probes labeled by 1, 6-hexandediamine and triethylenetetramine can detect 0.1-0.5pg target DNA in dot blot hybridization, and their sensitivities are better than the others.

本文详细考察了乙二胺、己二胺、二乙烯三胺、三乙烯四胺、四乙烯五胺及合成的聚乙烯胺化合物对λDNA探针的标记和检测的影响，发现胺化物链长和空间位阻影响探针的杂交和检测，用己二胺和三乙烯四胺标记的生物素探针在杂交24小时、显色8小时后检测灵敏度达0.1-0.5pg，超过其它胺化物标记的探针。

Therefore, many problems in the anorectal area are labeled as hemorrhoids, Dr.

因此，肛肠区的许多问题都被认为是痔。

All major neuropil regions are labeled by 5-HT antiserum except the optic lobes, antennal lobes and calyces of mushroom bodies, which are almost devoid of 5-HT immunoreactivity.

除常规的免疫阳性神经元(仅细胞质被标记的神经元和整个胞体都被标记的神经元)外，我们在前脑中部及触角叶内侧还发现了一些只有细胞核对 5-HT 抗血清发生反应的特殊神经元。

The thesis is concerned with the fermentation of yeast Candida arborea AS2.566, separation and purification of L-tryptophan and synthesis of 15N labeled anthranilic acid.

本文以发酵法生产L-色氨酸为背景，重点研究了由假丝酵母AS2.566获得L-色氨酸的发酵条件、色氨酸的分离纯化工艺以及邻氨基苯甲酸的合成过程。

The optimum pH for labeling was between 7.5-8.0, and the optimum spiking rate of polyclonal antibody was 12μg/ml. The absorb pad, sample pad, glass fiber of ARIST and nitrocellulose membrane HF24004 of Millipore were chosen as GICA materials. The carbofuran-OVA conjugate 0.97mg/ml and sheep anti-rabbit IgG 0.48 mg/ml was dispensed with Nano-Plotter (NP 1.2) onto the NC membrane to serve as test line and control line, respectively. The colloidal gold-labeled anti-carbofuran antibody was dispensed on the glass fiber with 20μl/unit.

选用ARIST公司的吸水垫、样品垫、玻璃纤维纸和Millipore公司的HF24004硝酸纤维素膜作为试纸条材料，用三维喷点平台分别将0.97mg/ml的克百威-OVA结合物和0.48 mg/ml羊抗兔IgG间隔5mm平行定量喷涂固化于硝酸纤维素膜上作为测试线和质控线，将金标记抗克百威多克隆抗体以20μl/垫定量滴加于玻璃纤维纸上，干燥硝酸纤维素膜和玻璃纤维纸。

But GST= 0.156, Nm=1. 588. As a result, the foundation of Youyongchi Avicennia marina population was the result of the migration of hypocotyles and human factors.

这项工作可以为海岸防护林中新引进种类的判定以及为研究种群建立者效应方法的确定提供科学依据。

The two-dimensional CDRC-ADI-FDTD update equations for collision unmagnetized plasma are induced. The unconditional stability of the CDRC-ADI-FDTD formulation for collision unmagnetized plasma is obtained by the examples.

推导了碰撞非磁化等离子体中的二维CDRC-ADI-FDTD迭代公式，并用算例验证了碰撞非磁化等离子体CDRC-ADI-FDTD算法也是无条件稳定的。