查询词典 knockdown

Knockdown of the gene blocked neural differentiation and the effect was rescued by the wild-type human gene, and not by a catalytically inactive mutant.

研究还发现，KDM7A表达抑制所引起的干细胞神经分化受阻现象可被FGF4的过表达所逆转，表明FGF4作为被KDM7A调控的基因介导了KDM7A在胚胎干细胞神经分化中的功能。

Methods RNAi combinant adenovirus vector which targeted 1566 site of NF-κB p65 mRNA was constructed and the effect of p65 gene knockdown in HUVEC was detected by Western blot analysis.

构建针对NF-κBp65 mRNA序列1566位点的重组RNAi腺病毒表达载体，并利用RNAi腺病毒抑制HUVEC p65表达。

This knockdown knife which has been processed through 306 times demandingly, advancedly and specially, has 17 parts and over 32 functions; its weight is just 216 kilos and it has been appointed to be the necessary equipment taken with the astronauts doing space missions by NASA.

这款由17种零件组成，具有多达32项功能的组合式刀具，经过306道严格先进的特定工序加工制成；刀组仅重216克，被美国国家航空航天局指定为航天员升空执行任务时的随身配具。

Combining virtual design and simulation, the conflict had been found on the design process, the paper made the conceptual design by Beitz method, function principle and design process model of key parts were built. By the emanative innovative design, the key parts' innovation and the knockdown conceptual prototype that was satisfied to function principle were obtained.

结合虚拟设计及仿真手段对甘蔗收获机的关键部件进行概念设计，在设计进程中找到冲突，通过采用构思—设计法构建关键部件的功能原理解和设计过程模型，并由此进行定向发散性设计，得到能解决冲突的关键部件的创新设计以及构建组合式的满足功能要求的整机概念模型。

After 44 h IVM, MOS level in the immatured ooeytes was higher than matured oocytes, and there was less MOS in nucleus than in the cytoplasm at 6 h after matured oocytes were activated; RNAi was performed before IVM, and all the three siRNA applied could successfully knockdown the level of c-mos mRNA, which were 0.08 ± 0.03, 0.11 ± 0.06 and 0.20± 0.06 times of the control group respectively, and the quantity of MOS was obvious decreased compared with the control group at the same stage.

本实验通过RT- PCR、免疫荧光激光共聚焦检测方法检测了猪卵母细胞在体外成熟培养过程中c-mos基因在转录水平、翻译水平上的表达以及蛋白的分布，并应用注射小干扰RNA方法对其进行了RNA干扰研究。

There has been a research indicating that geminin overexpression or cdt1 knockdown decreased the number of mitotically active cells and this effect depended on direct interaction between Geminin and Cdt1 .This balanced interaction of Geminin and Cdt1 is critical for the cell-cycle regulation.

在斑马鱼中研究发现，过量表达Geminin或者降低Gdt1的表达能减少有丝分裂的细胞数量，并且这种对细胞周期的效应直接取决于Geminin-Cdt1的相互作用，总之，Geminin和Cdt1的相互作用对细胞周期的调节非常重要。

Inhibition of PKCdelta by rottlerin or knockdown of TG2 protein by a TG2-specific siRNA resulted in a marked increase in autophagy shown by presence of autophagic vacuoles in the cytoplasm, formation of the acidic vesicular organelles, membrane association of microtubule-associated protein 1 light chain 3 (LC3) with autophagosomes, and a marked induction of LC3-II protein, important hallmarks of autophagy, and by electron microscopy.

抑制PKCdelta的rottlerin或击倒的TG2蛋白质的TG2特定的siRNA导致显着增加，表明自噬存在自泡在细胞质，形成酸性囊泡细胞，膜协会微管相关蛋白1轻链3（ LC3 ）与autophagosomes ，并显着诱导LC3 -Ⅱ蛋白的重要标志吞噬，并通过电子显微镜。

When their fermentation broths were diluted 5-fold, the strains DA07041 and DA07118 had higher knockdown efficiency, respectively 72.1%,and 83.7%.

发酵液稀释5倍后对线虫的击倒效果最好的是：DA07041和DA07118，其校正击倒率分别为72.1%和83.7%。

These potential problems were rapidly addressed by others who found that chemical modifications of the siRNA sugar backbone could block most off-target effects without jeopardizing gene knockdown.

这些问题很快就有人研究清楚：对siRNA的糖骨架进行化学修饰可阻止大部分的脱靶效应，而不影响基因&敲低&的效果。

I have therefore generated several p130cas RNA interference transgenic lines to determine the possible function of p130cas during neurogenesis. Knockdown of p130cas expression in developing neurons resulted in partial fusion in the commissures axon fascicles of CNS, photoreceptor axon targeting and guidance defects, and bulged synapse boutons at the neuromuscular junction. Moreover, reduced expression of p130cas in sensory organ precursor cells and proneural clusters resulted in bristle development defects.

为了了解p130cas的功能以及更进一步的分析p130cas在神经发育过程所扮演的角色，我建立一系列可以执行RNA干扰技术的转殖果蝇，结果显示在果蝇的神经中降低p130cas的表现会造成腹部神经索中原来排列整齐的commissure axons神经束偶尔会出现不正常的融合；若将p130cas专一地在果蝇眼睛中降低则会造成果蝇视神经突触目标辨识与突触导向的缺陷；而如果局部的运动神经系统中降低p130cas的表现会造成在神经肌肉接点的神经突触有澎大的现象；此外在果蝇的感觉器官前细胞以及原神经细胞丛，降低p130cas的表现则会导致刚毛发育的缺陷。

He and Nina moved to California and lived at 2005 Ivar Street, Apt.

他和Nina搬到加州，并在2005年伊瓦尔街，公寓生活。

Droperidol （ potently inhibits transfected HERGchannels and this is the probable mechanism for QT prolongation.

氟哌利多有效地抑制了转染的 HERG 钾通道，可能是 QT 间期延长的机制。