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We have employed a two-colored fluorescent reporter system to isolate FHF and SHF progenitors from developing mouse embryos and embryonic stem cells.

迄今之前，科研人员无法做到的一件事就是如何诱使细胞形成假设能在患病的心脏中行使功能的健康心肌。

The Chinese refused, as Japanese control of the bridge and Wanping town would completely isolate Beijing.

中国坚决反对，因为日本如果控制了宛平镇和卢沟桥，就等于把北京与外界完全隔绝了。

The result of study indicated: the isolate that was obtained in the test was pathogen causing tomato Verticillium wilt, and it was Verticillium dahiea kleb .

根据供试菌株在鉴别寄主上的反应，可以确定本试验分离并利用的番茄黄萎病菌为生理小种1(Vercitillium dahiea race 1)。

Mothods: Cock embryo was used to isolate influenza viruses, and the virus titer was determined through hemagglutination test. The gene was amplified by RT-PCR technique from the isolated strain and sequenced, and the gene phylogenetic tree was analyzed.

采用鸡胚进行病毒分离，采用血凝试验初步确定病毒效价，用逆转录聚合酶链反应法扩增分离株的部分片段后进行克隆和测序，然后进行进化树分析。

The characterestics of these strains identified from the artifically infected soft-shelled turtle are identified the primary isolate.

从感染鳖的肝脏中分离到与原分离株形态及生理生化特性相同的菌株。

I used the homemade ECDL to measure the 2S-3D two photon transition spectrum of Helium , the advantages are that the expense of homemade ECDL is cheap and have good output power. At the same time, I used the electronic part can be got in the market to manufacture a pulse generator in the experiment, the pulse generator can control the output frequency, the pulse width, the relative phase and also can simultaneously control four sets of systems or instruments need the TTL pulse control. The advantage is that the cost of the pulse generator is cheap and the pulse generator is stable. To isolate the magnetic field I used the magnetic foil to helium cell. I also built a gas discharge system, the switch sequence of this system can be controlled by the pulse generator. Finally I built a detecting system can measure the helium energy state. It is cheap and can be combined with the comb laser system in my laboratory.

本实验是利用一台自制的外腔雷射系统来量测2S-3D的氦原子双光子跃迁，其优点在於自制的外腔雷射之费用非常便宜，也有不错的输出功率，同时在实验中我利用市售的电子零件制作了一台脉冲产生器，可控制输出的频率、脉冲宽度以及相对的相位，且可同时控制四套需要TTL脉冲控制的系统或仪器，优点除了便宜之外，也非常稳定；为了隔绝磁场，利用了磁场隔绝金属箔，我也在实验室中建立了一组气体放电系统，其系统可以用脉冲产生器来控制开关时序，最后，我建造了一个氦原子能阶的量测系统，而且费用低廉，可与本实验室的另外一门研究光梳雷射系统做结合。

I used the homemade ECDL to measure the 2S-3D two photon transition spectrum of Helium , the advantages are that the expense of homemade ECDL is cheap and have good output power. At the same time, I used the electronic part can be got in the market to manufacture a pulse generator in the experiment, the pulse generator can control the output frequency, the pulse width, the relative phase and also can simultaneously control four sets of systems or instruments need the TTL pulse control. The advantage is that the cost of the pulse generator is cheap and the pulse generator is stable. To isolate the magnetic field I used the magnetic foil to helium cell. I also built a gas discharge system, the switch sequence of this system can be controlled by the pulse generator. Finally I built a detecting system can measure the helium energy state.

本实验是利用一台自制的外腔雷射系统来量测2S-3D的氦原子双光子跃迁，其优点在於自制的外腔雷射之费用非常便宜，也有不错的输出功率，同时在实验中我利用市售的电子零件制作了一台脉冲产生器，可控制输出的频率、脉冲宽度以及相对的相位，且可同时控制四套需要TTL脉冲控制的系统或仪器，优点除了便宜之外，也非常稳定；为了隔绝磁场，利用了磁场隔绝金属箔，我也在实验室中建立了一组气体放电系统，其系统可以用脉冲产生器来控制开关时序，最后，我建造了一个氦原子能阶的量测系统，而且费用低廉，可与本实验室的另外一门研究光梳雷射系统做结合。

The isolate Paecilomyces farinosus sw03032 was cultured in a same liquid medium at 25℃ and the impact of rotate speed of rocking bed,initial inoculum and initial pH value on mycelial bioass were studied.

在25℃下同一种培养液中，研究了摇床转速、接种量和初始pH值对粉拟青霉菌丝生长量的影响。

The most pathogenic isolate, VIB 645, contained two closely related hemolysin genes, which were cloned and sequenced. In this study, vhhA was cloned into an expression plasmid pET-24d.

本研究将哈维氏弧菌的溶血素基因vhhA克隆到大肠杆菌的表达载体pET-24d，VHH溶血素作为一种带六个组氨酸的融合蛋白在大肠杆菌表达菌株BL21(DE3)中得到了过量表达，重组大肠杆菌及其培养上清液在鱼血平板上有很强的溶血活性。

Long telephotos 300mm and longer need not be left at home, they too have their place in landscape photography. With their depth compression and narrow angle of view they can isolate and showcase the subject of your choice.

长焦300mm和更长的不必闲放在家里，他们在风光摄影中太有位置了，用他们的压缩景深和窄角，他们可以分离或显示你选择的物体。

Chinese traditional virtue is humility and wariness,the compliment and praise to the others should be declination

中国人的传统美德是谦虚谨慎，对别人的恭维和夸奖应是推辞。如

We bought this house on the never-never.

我们以分期付款的方式买下这座房子。