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To isolate spermotogonia using two combinatorial enzyme three step wises digest the testicular tissues from 19 d-old-embryo after hatching,the cryopreserve effect of dimethylsulphoxide,glycerol and ethylene glycol cryoprotectants,each at(5%,10%,and 15%) concentration on the 19 d chicken embryonic SSCs was compared with fast freeze method.

采用二酶3步法获取孵化第19 d的鸡胚精原干细胞，比较在快速冷冻条件下3种冷冻保护剂(DMSO、乙二醇、甘油)在3个浓度5%、10%、15%条件下对鸡胚精原干细胞的冷冻保存效果进行研究。

Anguillarum isolates, while 94.3% and 91.9% nucleotide identical to L. pelagius and Photobacterium damselae respectively. MP analysis showed that ayu-H080701 shared 97.6%-98.8 % amino acid sequence identical to L. anguillarum isolates, while lower than 75.6 % to other bacteria. Phylogenetic analysis showed that ayu-H080701 grouped constantly with L. anguillarum isolates. The biochemical, physiological tests and sequence analysis all strongly supported the identification of the pathogen causing ayu vibriosis in Ninghai country, China, as an isolate of L.

PCR扩增检测表明，细菌16S rRNA 基因通用引物和鳗利斯顿氏菌MP基因特异引物均能扩增到预期大小的特异性条带。ayu-H080701与鳗利斯顿氏菌16S rRNA基因核苷酸序列同源性最高，为99.4%～99.5%，与同属的海弧菌和美人鱼发光杆菌分别为94.3%和91.9%；ayu-H080701与鳗利斯顿氏菌MP氨基酸序列同源性高达97.6%～98.8 %，与其它弧菌科成员则低于75.6 %，系统进化树分析也揭示ayu-H080701与鳗利斯顿氏菌进化相关性最高。

Among these four varactors, two of them are fabricated on n-well and use the p-n junction between n-well and substrate to isolate substrate noise. The other two are fabricated on p-well and surrounded by deep n-well and n-well in order to isolated substrate noise.

这四种变容器中，其中两种生成於n型井上且利用介於n型井与基体的p-n接面隔绝基体杂讯；另外两种生成於p型井上且被深层n型井与n型井所包围以隔绝基体杂讯。

Phylogenic analysis based on entire S1 gene sequences showed that majority isolates clustered together with the close phylogenetic relationships. Therefore, we researched pathogenicity of the selected isolate IBV CK/CH/LDL/05II in this group, Pathogenic study showed that this virus caused 100% morbidity and 54.5% mortality to 15-day-old specific-pathogen-free.The kidneys from the dead chickens showed the predominant gross lesions which were pale, mottled, and swollen. The renal tubules and ureters were distended with excess urates.

经S1基因系统进化树分析发现，本实验所分离的大多数IBV毒株(8/12)分布于同一基因进化群内，并且亲缘关系较近，因此，我们对此群中IBV毒株CK/CH/LDL/05II的致病性进行研究，以10~(5.5)×EID_(50)/0.1ml的病毒接种15日龄的SPF雏鸡，鸡群发病率为22/22(100%)，死亡率为12/22(54.5%)，死亡鸡以肾脏肿大、苍白、外观呈现典型的&花斑肾&，肾小管和输尿管扩张并且有尿酸盐沉积为主要病理变化。

From factorial experiments, it was observed that in conventional extrusion treatment, defatted soy flour, aqueous acidic leached soy protein concentrate and soy protein isolate was able to be texturized, whereas SPC was not.

通过挤压实验可知，在常规的挤压条件下，低温粕、酸法SPC和大豆分离蛋白均可实现组织化，而醇法SPC无法实现有效的组织化。

Method］ Solid and liquid medium of the nitrogen-free BAP, S, JA, ISP4, CB-CcI3, DPM, FTW were used to isolate and purify root nodules of Elaeagnus and Hippophae collected from Tongliao and Chifeng in Inner Mongolia. According to determination results of 16S rDNA sequence, a phylogenetic analysis was carried out.

方法］以通常用于分离弗兰克氏菌属固氮放线菌的无氮BAP、S、JA、ISP4、CB-CcI3、DPM和FTW等为固体和液体培养基，对采集自内蒙古通辽和赤峰的沙棘和沙枣植物根瘤进行放线菌的分离纯化，并依据16S rDNA序列测定结果对分离物进行系统发育分析。

By using these computer-enhanced EEGs, scientists can identify and isolate the brain's low-amplitude "emotion signature clusters", synthesise them and store them on another computer.

通过用这些计算机增强脑电波，科学家能辨别和孤立大脑&向下振幅&的情绪信号串，综合它们并存储他们在另一台电脑。

By using these computer-enhanced EEGs, scientists can identify and isolate the brain's low-amplitude "emotion signature clusters", synthesise them and store them on another computer.

通过用这些计算机增强脑电波仪，科学家能辨别和独立出大脑&低幅&的情绪信号串，综合它们并将他们存储在另一台电脑中。

With Advanced Segments, you can quickly isolate and analyze subsets of your traffic.

用先进部分，您可以快速地分离和分析亚群的流量。

A viral isolate was obtained from naturally infected sheldrakes liver using free of maternal antibodies.

应用无母源抗体的鸭胚从疑似病毒性鸭肝炎的病麻鸭肝脏中分离到1株病毒。

Chinese traditional virtue is humility and wariness,the compliment and praise to the others should be declination

中国人的传统美德是谦虚谨慎，对别人的恭维和夸奖应是推辞。如

We bought this house on the never-never.

我们以分期付款的方式买下这座房子。