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Comparison from the Germany isolate, phocine distemper virus type Ⅱ(PDV-2) and vaccine strain, the major difference is the long signal peptide domain while the mature protein exhibit high identity, and all of the 13 serine residues, four potential asparagine glycosylation sites and two hydrophobic regions supposed to affect the fusion function are completely identical. These data supports the view that F protein is more conservative than H protein in CDV.

三、犬瘟热病毒中国分离株囊膜糖蛋白基因免疫小鼠诱发的抗体应答经一系列步骤将CDV-YZ0101株的两囊膜糖蛋白基因定向导入真核表达载体pcDNA3.1中，DNA测序和酶切分析筛选阳性重组质粒克隆pcDNA-H和pcDNA-F，以重组质粒DNA和脂质体共转染COS-7细胞，用间接免疫荧光试验证实转染的COS-7细胞的胞浆中分别表达了CDV的H和F蛋白。

The first layer is phosphosilicate glass and the second layer is silicon nitride. Now the passivation layer can isolate the device circuits from external contamination damage.

在护层的薄膜中，经常使用双层薄膜(Multi-Layer)的结构，第一层薄膜沈积磷矽玻璃，第二层薄膜则沈积氮化矽，即可将元件线路结构与外界污染物隔离。

Nucleotide sequence analysis identified 2 strains with 93.4% identity, phylogenetically most closely related to the Greece1 isolate.

核酸序列分析证实2株有93.4%的同源性，种遗传性上与希腊种最为相近。

The purpose of this study is to screen microorganisms with growth inhibition against plant fungal pathogens. The plate-dilution method was used to isolate the bacteria from salt marshes at Dafeng, Jiangsu. The antifungal activity of the bacterial broth filtrates against 5 phytopathogens was determined using mycelial disk method. The antifungal compositions of four active bacteria were analyzed primarily with chromatography techniques. Two hundred and thirty-five aerobic (92) and anaerobic (143) bacterial strains have been isolated from the coast salt marsh and sixty-five strains, of which 39 are aerobic and 26 are anaerobic showed greater than 30% of inhibition rate against the growth of at least one of the phytopathogenic fungi on petri dish.

为了开发对植物病原真菌生长有抑制作用的药源微生物，本实验采用平板稀释法从江苏大丰盐沼湿地土壤中分离微生物，并通过菌丝块法检测这些微生物代谢产物对5种植物病原真菌生长的抑制活性，进而利用柱层析分析其活性成分；结果共分离得到235株细菌，其中好氧细菌92株，厌氧细菌143株；体外活性测试结果显示有65株至少对1种测试植物病原真菌的抑制率在30%以上，其中好氧菌39株，厌氧菌26株。

Guanidine Thiocyanate method was used to isolate total RNA from the inflorescence tissue and leaves of Platanus aceriflia successfully with regard to high quantity of phenolic compounds in its tissue.

从植物组织中提取纯度高、完整性好的RNA是进行Northern杂交、RT-PCR、cDNA文库构建和差别显示分析等分子生物学研究的关键。

When attacked by this isolate, the color of young Porphyra gametophytes turned green.

当遭到此菌侵害时，幼苗期的紫菜配子体的颜色变绿。

We intend to isolate and screen a strain of Propionibacterium acnes from health cow rumen fluid, and study its rumen fermentation characteristics.

本研究旨在从健康奶牛瘤胃液中分离、筛选出以产丙酸为主的痤疮丙酸杆菌，研究其瘤胃发酵特性。

Microscopically, globular or ovoid cells, a number of round, ovoid shaped endospores could be observed. No hypha, ascus or blastic conidia was found upon cultivation on SDA. Based on the morphological characteristics, this isolate could be identified as Prototheca species.

结果 该菌在25℃、37℃均可生长，沙堡弱培养基、马铃薯培养基见光滑湿润的白色酵母样菌落，镜下圆形、椭圆形的孢子及裂殖成圆形或不规则形的内孢子，无菌丝、子囊、芽孢，根据形态学特征鉴定为无绿藻。

Yeast two hybrid and TAP will be utilized to isolate the Pygopus binding proteins so that we elucidate the mechanisms of Pygopus in Wnt- dependent and independent pathways.

我们将利用酵母双杂交和TAP技术分离pygopus的结合蛋白，以彻底阐明其作用机理。

C An attempt is initiated to separate a mixture of different forms of the same molecule and isolate a desired form of the molecule for pharmacological or other reasons (e.g., separation of racemic mixtures).

尝试分离相同分子的不同形态的混合物，并隔离出药用分子的想要获得的形态。或者其它原因（如，消悬体混合物的分离）。

Objective:To compare the response control and attention of Schizophrenic patients with that of the healthy controls by Integrated Visual and Auditorycontinuous performance test. To quantitate the impairment of cognitive function in patients, and to explore the relationship between cognitive function and the severity of the disorder.

目的：探讨精神分裂症病人在IVA持续操作测试中的反应控制能力、注意力等，并与健康人进行比较，从而量化精神分裂症病人的认知功能损害，并进一步探讨其认知功能与疾病严重程度的关系。

Main effective factors including subcooling degree, mechanical vibration, gas hydrate reformation, environment temperature, noncondensing gas and surfactant are analyzed.

指出过冷度、机械振动、重复生成水合物、环境温度、不凝性气体、添加剂是影响气体水合物生成的主要因素，还对R152a水合物的放冷进行了实验研究。