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Anti-histidine-tag antibody is usually used for identification of expression of the recombinant protein expressed using pRSET-A. A pair of primers that contained flanking hydropathic amino acid codons were synthesized, to amply the sequence coding for 10~34 aa in the precursor sequence.

质粒pRSET-A是一个常用的高效原核表达载体，编码一N端含组氨酸标签（6×His）的34aa前导肽序列，以方便利用抗组氨酸标签抗体鉴定或纯化所表达的重组蛋白。

A pair of primers that contained flanking hydropathic amino acid codons were synthesized, to amply the sequence coding for 10~34 aa in the precursor sequence. The amplified fragment was inserted into pRSET-A to generate the first double repeat of the precursor gene. By utilizing a pair of isocaudamer BamH I and Bgl II sites, and another downstream Hind III site of plasmid pRSET-A, following a series of simple double digestions and ligation of the resulted products, a series of repeat (3, 4 and 6) precursor peptide fragment genes were derived.

本实验设计一对两侧含编码疏水性氨基酸密码子的引物，经过扩增前导序列10~34aa基因序列，并重新克隆入质粒pRSET-A构建串联二聚体后，再利用质粒pRSET-A的BamH I / Bgl II同尾酶克隆位点，经一系列简单的酶切和连接，快速构建这一前导肽中不含组氨酸标签序列的串联多聚体基因，并成功表达其六聚体重组蛋白。

This thesis has made a deep study on lacustrine sequence stratigraphy and its application to oil and gas exploration and development, based upon the latest theory of sequence stratigraphy, limnology and petroleum geology incorporated with sedimentology, geophysics, tectonics, geochemistry, paleontology, paleoecology, ichnology, paleogeomagnatics and mislaneous analytical means.

"济阳坳陷下第三系沙河街组油气层序地层学研究"以层序地层学、湖泊学和石油地质学的最新理论为指导，充分利用丰富的地震、测井、岩心、录井和油藏工程等资料，综合沉积学、地球物理学、构造学、地球化学、古生物学、古生态学、遗迹学、古地磁学及多种分析测试手段对湖泊层序地层学及其在油气勘探开发中的应用进行了深入探讨。

The method applied in this paper uses the identic sequence instead of chirp sequence.

与以往采用chirp序列作为训练信号的方法不同，该文采用全序列作为训练信号。

Its website provides the introduction of this center, information of achivements, research areas, resources of genetics, related news, databases (includes GenBank Nucleotide Sequence db, DNA Data Bank of Japan, Genome Sequence db, Homologous Vertebrate Genes db, Immunogenetics db and so on).

网站提供该中心介绍，成果信息，研究领域，遗传学资源，相关新闻，数据库（包括基因银行核苷序列数据库，日本DNA资料库银行，染色体序列数据库，同源脊椎动物基因数据库，免疫遗传学数据库等等）。

Now, we can make the sequence diagram for each implemental method, if required, to describe its algorithmic sequence of signals.

现在，我们可以为每个而推行方法的序列图，如果需要的话来描述其算法的信号序列。

Base on the analysis, an improved training symbol with symmetric and repetitive structure is proposed, and the estimate range of the frequency offset is enlarged by increasing the number of repetitive sequence and shortening the interval of repetitive sequence.

在此基础上，提出了一种改进的训练符号结构，即在SC算法重复结构的训练符号中引入共轭对称结构，同时通过增加重复序列的数目，缩小重复序列之间的间隔，进一步扩大基于单个训练符号的频偏估计范围。

The increasing sequence in compliance with modular hierarchy number was speed optimizing sequence of minimum delay time, fixed resource expense and power dissipation in all cascade sequences of hybrid modules of TC2CLA.

并得出和证明了按模块层数递增级联序列是混合模块TC2CLA各序列中延迟时间最短、资源占用与功耗不变的速度优化序列。

In the full-scale aero-elastic model test, it is observed that there exist more than one interval of poor aerodynamic performance, and the non-symmetric erection sequence, while increasing the aerodynamic stability at the very places where aerodynamic performance of symmetric erection sequence is poor, performs worse in some other intervals with a vertical flutter phenomenon.

在全桥气弹模型的风洞试验中发现，采用对称架梁施工方法，在施工过程中会存在颤振稳定性最差的区间，并且这样的区间可能不止一个，而采用非对称架梁施工方法，固然在那些采用对称架梁施工时颤振稳定性较差的区间处提高了颤振稳定性能，但却在另一些施工区间中表现出比对称架梁施工更差的颤振稳定性能。

In essence, stream cipher is such a pseudorandom generator that its chaos sequence of output and random sequence are computational indistinguishability; and the aim in designing block cipher materially is to design a pseudorandom permutation which is computationally indistinguishable from random permutations.

实际上，序列密码本质上就是一个伪随机生成器G，其输出的乱数序列和随机序列是计算不可分辨的；而分组密码的设计目标实质上就是设计一个伪随机置换，它与随机置换是计算不可分辨的。

Bob Eckert has been chairman and chief executive of Mattel for eight years.

美泰董事长兼首席执行官鲍勃·埃克尔特在任已8年。

The outlook for honors and disgraces carries on and surpasses the traditional outlook for honors and disgraces theoretically, and carries on and develops the theory of construction of socialist mora...

社会主义荣辱观的提出在理论上是对传统荣辱观的一次继承和超越，是对社会主义道德建设理论的继承和发展，在实践上有利于形成良好的社会风尚。