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Results 500fold enrichments were observed by tittering phages in the cell lysate after five rounds of selection. A signal significantly greater than background binding was observed from the third to the fifth round of selection on CHOEGFRGFP1 and CHOK1 cell, but a part of polyclonal scFv bound EGFR specially. About 45% of the selected clones contained a fullsized insert of 1 kb. One unique human antiEGFR scFv (F4scFv) was isolated by analyzing with cell ELISA and DNA sequencing.

结果 经过5轮筛选，细胞裂解液中洗脱出噬菌体效价有500倍以上增长；细胞ELISA结果显示多克隆pscFv与CHOEGFRGFP1细胞和CHOK1细胞均有明显结合，但有部分特异性结合EGFR；菌落PCR显示约45％克隆中含有完整的1kb scFv片断；经细胞ELISA、 DNA测序检测共获得1株EGFR特异性单链抗体，命名为F4scFv。

This allows the crab to insert a large toothlike appendage on its right-hand claw into the opening, thus cracking the snail's shell.

这样就可以把它右爪那边象牙齿样的东西刺进蜗壳口从而弄碎蜗壳。

Methods In 19 patients with nonmalignant tracheoesophageal fistulas in intensive care unit, 9 patients accepted PEG tubes inserted by push technique. The remaining 10 patients accepted the insert of self-expandable metallic stents and the stents were extracted 2 months later with the complete mucosal healing confirmed by endoscopy and typical swallow-cough sign.

19例良性气管食管瘘患者，9例行PEG留置造瘘管、10例行内镜下食管覆膜自膨式金属支架植入术，2个月后经内镜将金属支架取出，以吞咽—咳嗽征检测气管食管瘘是否完全愈合。

The shuttle-shape and uplifted composite tissues in the middle of residual ear were cut ,and then insert into the vertical incision in the place which the tragus will be made .

根据残耳组织的形状，切取一定大小的梭形，中央略隆起的残耳复合组织，在拟再造的耳屏部位作纵向切口，将残耳复合组织插入切口内，缝合即可。

The shuttle-shape and uplifted composite tissues in the middle of residual ear were cut ,and then insert into the vertical incision in the place which the tragus will be made. ResultsAll the tragus reconstructed existence perfectly, none of them suffered from necrosis.

根据残耳组织的形状，切取一定大小的梭形、中央略隆起的残耳复合组织，在拟再造的耳屏部位作纵向切口，将残耳复合组织插入切口内，缝合即可。

The shuttle-shape and uplifted composite tissues in the middle of residual ear were cut ,and then insert into the vertical incision in the place which the tragus will be made. Results All the tragus reconstructed existence perfectly, none of them suffered from necrosis.

根据残耳组织的形状，切取一定大小的梭形、中央略隆起的残耳复合组织，在拟再造的耳屏部位作纵向切口，将残耳复合组织插入切口内，缝合即可。

The 682-bp fragment of human IFN-〓 cDNA was inserted into theXhoI site of the BMGNeo vector containing a neomycin resistancegene.The plasmid containing the translationally oriented IFN-〓cDNA insert (BMGNeo-IFN-〓) was selected by restriction enzymeanalysis.

将682bp的全序列人〓 cDNA插入到携有〓基因的表达载体BMGNeo的Xho I位点上，对此重组表达载体进行了限制性酶切鉴定。

The lower two-thirds of it insert, in common with fibers of the external oblique and the underlying transversus abdominus, into the

它的下三分之二和腹外斜肌以及内面的腹横肌纤维附着于

Either undock or insert an appropriate adapter.

取消对接或插入相应的适配器。

Either undock or insert an appropriate adapter.

您的电源适配器功率不足以运行连接的对接站。

Don not attempt to do something which you can not to do.

不要企图做那些办不到的事情。

The expression of CTGF and TNF-αweredetected by immunochemistry and the number of Clara Cells was calculated.

光镜下观察肺组织的病理变化，采用免疫组化染色观察肺组织中结缔组织生长因子和肿瘤坏死因子-α的表达和Clara细胞的数量。