查询词典 insert

I pull the wicker pots up slantingly, the dark green lobsters aredesperate with their claws as I take them out, I insert

我那一伙成人或半成人的青年们，他们和任何别人在一起都不如和我在

Methods Digest recombinant plasmid pU57-SOD with SnaB Ⅰ and Not Ⅰ and insert the obtained SOD gene into expression vector pPIC9k.

方法根据酵母密码子偏好性，合成人Cu，Zn-SOD基因，克隆至真核表达载体pPIC9k中，转化毕赤酵母GS115。

Methods Amplify IL-6 (23)-PE40 gene from the previously constructed recombinant plasmid pKK-IL-6(23)-PE40 by PCR, and insert to the SnaB Ⅰand NotⅠ sites of vector pPIC9. Transform the constructed recombinant plasmid pPIC0-SN to P. pastoris GS115 by electroporation, screen Mut-recombinants for expression under induction of methanol.

采用PCR技术，将目的基因IL-6(23)-PE40插入到pPIC9载体中SnaBⅠ与NotⅠ位点，电转化至巴斯德毕赤酵母GS115中，筛选Mut-型重组酵母；甲醇诱导表达，体外细胞试验检测其细胞毒性。

Be sure to insert the correct name of your friend when you tell them "snaps."

当你叫你的朋友弹指的时候，确保你投入的是你朋友的名字没错。

The high price of wild C. sinensis has led unscrupulous harvesters to insert twigs or even lead wires into the stromata, thereby increasing the weight and price paid. Cultivated C.

野生虫草的高价导致不道德的采集者在基质中灌入细枝或者甚至是铅线，因此增加了重量和价格支付。

Results: This study established a web-based automatic syndromic surveillance system which could utilize the Internet to upload the data files, implement statistical analysis regularly, insert new data into database automatically , offer various types of statistical graphs and GIS (geographical information system) maps, and present the latest epidemic situation materials on the webpage.

研究结果：本研究建置之症候群监测系统，利用网际网路上传资料档案，定时排程执行统计分析，即时更新资料库内容，透过网页呈现最新的疫情资料统计图表，透过多重的查询介面，防疫工作人员能藉此对异常警示作更进一步的分析与确认，降低疫情误判的发生。

Removing the PGE2 vaginal insert will usually help reverse the effects of the hyperstimulation and tachysystole.

PGE2阴道制剂的取出通常有助于逆转子宫过度刺激和收缩过速。

Another pair of primers including the start codern and the terminer coden were designed to amplify the PAL gene. Two restriction enzyme XbaI and XmaI were added to the two primers respectively, which enable to insert this fragment to the prokaryotic expression vetor pCAMBIA2301-WCS.

在PAL 基因的编码框以启始密码子ATG 和终止密码子TAG 为两端设计一对特异引物，分别在引物5' 端引人XbaI 和XmaI 切位点，用来扩增PAL 基因并与pCAMBIA2301-WCS 构建表达载体pCAMBIA2301-PAL。

Another pair of primers including the start codern and the terminer coden were designed to amplify the PAL gene. Two restriction enzyme XbaI and XmaI were added to the two primers respectively, which enable to insert this fragment to the prokaryotic expression vetor pCAMBIA2301-WCS.

在PAL 基因的编码框以启始密码子ATG 和终止密码子TAG 为两端设计一对特异引物，分别在引物5' 端引人XbaI 和XmaI 酶切位点，用来扩增PAL 基因并与pCAMBIA2301-WCS 构建表达载体pCAMBIA2301-PAL。

The efficiency of selection was monitored by comparing the number of phage recovered from the acid elution and cell lysate in each round,and by testing EGFR binding specificity of polyclonal phagescFv on CHOEGFRGFP1 and CHOK1 cell with cell ELISA. Bacterial PCR was used to select clones containing a 1 kb insert. Cell ELISA was used to determine EGFR binding specificity of monoclonal pscFv on EGFR positive and negative cell. The number of individual EGFRbinding clones was determined with nucleotide sequencing. Results 500fold enrichments were observed by tittering phages in the cell lysate after five rounds of selection.

以稳定转染的CHOEGFRGFP1细胞和未转染的CHOK1细胞分别作为EGFR阳性和阴性细胞，采用负筛选的方法进行筛选；通过比较每轮投入及洗脱出噬菌体的效价比以及细胞ELISA检测多克隆pscFv与阳性、阴性细胞结合情况对筛选过程进行监测；采用菌落PCR挑选含有全长scFv片断的菌落，进一步用细胞ELISA检测单克隆pscFv与EGFR阳性及阴性细胞结合特异性；挑选EGFR特异性单克隆pscFv采用DNA测序法确定克隆多样性。

Chimborazo and Cotopaxi, took me by the hand.

越过琴博腊索山和科托帕克西山。

This car is in a good condition.

这辆车的状况很好。