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Calcium ionophore-induced tryptase release was inhibited 60% and 69% by 10 and 50 microM of DL, respectively, and anti-IgE-induced tryptase release was inhibited 33%, 47% and 66% for 1, 10 and 50 microM of DL.

研究发现浓度分别为1、10和50mM的DL分别使钙离子载体诱导的LTC4释放下降29%、35%和39%，分别使IgE诱导的类胰蛋白酶释放下降33%、47%和66%。

Effects of Simvastatin on the Transdifferentiation of Renal Tubular Epithelial Cells Induced with High Glucose UANG Xiao-xia,KE Chang-bin,ZHANG Qing-hong,ZHANG Jian-e*（Department of Nephrology;Department of Anesthesia,Taihe Hospital,Yunyang Medical College,Shiyan,Hubei 442000,China）Abstract:Objective To investigate the effects of simvastatin in renal tubular epithelial cells transdifferentiation induced with high glucose.

作者：黄晓霞，柯昌斌，张庆红，张建鄂*作者单位：郧阳医学院附属太和医院肾病内科；麻醉科，湖北十堰 442000 目的：观察辛伐他汀对高糖诱导的肾小管上皮细胞转分化的作用。

Two special TL phenomena have been found in Yanzhuang meteorite through the determination of the natural TL, annealed TL and induced TL by β radiation: l.when the sample of Yanzhuang meteorite is annealed at temperature up to 500℃, the TL peak induced by β rays moves to lower temperature with the increasing of irradiation dose; 2.when the annealed temperature is greater than 600℃, the TL peak temperature of the annealed sample is higher than that of the unannealed sample.

991年10月31日21点45分在广东省翁源县岩庄乡境内发生了陨石降落。从降落现场收集到3.skg的陨石样品，经初步鉴定为H6型、具有强冲击变质特性的珍稀普通球粒陨石。根据陨石命名规则定名为岩庄陨石。普通球粒陨石是目前研究太阳系早期所发生的化学与物理变化过程的最好的信息来源。

The HSS action showed a dose-effect manner and this action was proved uneffective with the larger dose of CCl〓.(2) Hepatic histological findings indicated that CCl〓-induced hepatic lesions could been alliviated by HSS.(3) CCl〓-induced reduction of hepatic mitochondrial succinic dehydrogenase activity was restored by the treatment of HSS.

相反，如果CCl〓所用剂量过大，HSS将无抗损伤作用；(2)HSS可使CCl〓损伤肝组织的程度减轻，用肝组织切片可充分证明这一现象；(3)HSS可使CCl〓损害肝细胞线粒体琥珀酸脱氢酶的活性恢复，用组织化学法可得到证实。

In this study, We used longer DD primers in combination with a two-step PCR protocol to overcome these two problems in term of modified methods of Martin and Pardee.K562 erythroleukemia cell differentiation induced by HEMIN or PMA for 36 hours was used as erythriod or megakaryocytic differentiation model in vitro.Total RNAs were extracted from induced and uninduced K562 cells and were applied to mRNA differential display analysis.

为解决此问题，我们参照Martin和Pardee的改进方案［3］，建立了一种使用长引物，进行二步PCR的改进DDRT-PCR方法，以氯高铁血红素和肉豆蔻佛波酯诱导K562细胞36小时作为体外红细胞和巨核细胞分化模型，取诱导前后的细胞总RNA进行mRNA差异显示分析。

To get more information of the function of human Era on cell cycle, the complete control inducible mammalian expression system was used to express human Era mutant and analyzed cell cycle of uninduced (add 1% ethanol) and induced (10μM Pon A) cell lines by flowcytometer, the primary results demonstrated human Era mutant could inhibit apoptosis induced by ethanol, and Era S36N may stimulate the expression of Bcl-2 as detected by Western Blot.

在构建稳定高表达蜕皮激素受体基因的细胞株3V3的基础上，转染突变型人Era的表达载体进行压力筛选，对所得到的单克隆细胞株进行诱导与未诱导条件下细胞周期的分析，结果表明未诱导加酒精的对照细胞可以引起细胞凋亡，而Era诱导表达后可以显著抑制凋亡细胞的数量，Western表明Era S36N可以诱导细胞Bcl-2表达，提示人Era可能与细胞凋亡相关。

A 1: Uninduced supernant of cell lysate by sonication; 2: Induced supernant of cell lysate by sonication; 3: Uninduced precipitate of cell lysate by sonication; 4: Induced inclusion body of cell lysate by sonication; 5: Purified ULBP4 protein; M: Low molecular marker proteins.

2.3 体外NKG2D结合实验和细胞因子分泌实验间接ELISA检测结果显示：原核表达复性后的ULBP4能与人NKG2D结合，而带His标签的无关蛋白则不能与之结合，说明复性后的ULBP4仍能维持正确的天然构像。

ANP and CX43 began to express at 2nd week after induction and increased gradually,about 60% of the resulting myogenic cells were positive at 4th week after induction ,they were negative for uninduced cells.hMSCs'surface antigen profiles obtained by Flow Cytometry were negative for CD31\CD34\CD45 before and after induction,but CD90 expressed higher after induction while was weak positive before induction(P.05). Apotosis index was correlated with the cultural time after induction,The apoptosis rate of induced hMSCs was remarkably higher than control group(P.01),and the variation between groups was notable(P.05),the cell cycle analysis showed that the percentages of G_0/G_1phases were reduced significantly after induction. The expresstion levels ofβ-MHC and CTNT mRNA were undetectable before induction,began to increase at 1st、4th week after induction,reached the peak at 6th week and decreased after that,the expression of Bcl-2 and Bax mRNA varied regularly after treated with 5-azacytidine. hMSCs'resting membrane potential、range and rate of depolarization were heightened gradually after being induced.

结果：hMSCs诱导前为纺锤形，诱导后第2天部分细胞即开始发生形变，呈球形或短棒状，1周后胞浆中颗粒增多，约20～30%细胞边缘呈毛刷样变化；hMSCs表面抗原CD31、CD34、CD45在诱导前后差异无统计学意义，CD90未诱导时表达呈弱阳性，诱导后明显增高(P.05)；ANP和CX43在诱导前无表达，诱导后第2周开始表达且表达随时间逐渐增强，但CX43在诱导后第5周表达量开始降低。hMSCs诱导后凋亡指数随诱导后培养时间增加，低浓度诱导组低于标准浓度诱导组，组间差异有统计学意义(P.05)，G_0/G_1期细胞比例诱导后较对照组显著减少(P.05)；β-MHC和CTNT基因分别在诱导后第1周和第4周时表达开始增强，在第6周时均达到高峰，第8周时表达开始衰减，Bcl-2、Bax基因表达呈时间依赖性变化，hMSCs经诱导后随心肌样细胞特征的表达膜静息电位、去极化幅值和去极化速率逐渐增高。

Chicken IL-15(ChIL-15), Which was discovered in 2001, plays a main role in activating NK cells and CD8+ memory T cells, and may therefore have potential as a vaccine adjuvant.According to published ChIL-15 gene sequence, a pair of primers were designed and synthesized to clone ChIL-15 cDNA from ConA-activated chicken splenocytes by RT-PCR. Recombinant plasmid pUC19ChIL-15 carrying the ChIL-15 gene was constructed. The gene encoding mature ChIL-15 (mChIL-15) was amplified from pUC19ChIL-15 by PCR and cloned into pMD 18-T vector. After digested with Kpn I /Pst I , the mChIL-15 gene was subcloned into prokaryotic expression vector pPRoEX橦Ta and transformed into E.coli DH5a .The transformant was induced by IPTG, and the recombinant ChIL-15(rChIL-15) was expressed as a fusion protein with a histidine hexamer tag at the N-terminal end of the protein. Following expression, the protein was purified by ProBond?Nickel-Chelating Resin. The uninduced and induced protein lysates and the purified protein were separated by SDS-PAGE and transferred onto nitrocellulose membrane to identify rChIL-15 by Western blot. The rChIL-15 was used to immune the guinea pig to obtain rChIL-15 polyclonal antibody.

参考已发表的ChIL-15基因序列，设计合成引物，应用RT-PCR技术从刀豆球蛋白A活化的白来航鸡脾淋巴细胞中克隆ChIL-15 cDNA，将其与SmalⅠ酶切处理的pUC19载体连接，构建重组质粒pUC19ChIL-15；用PCR技术从pUC19ChIL-15中扩增出去信号肽的成熟ChIL-15(mChIL-15)基因，与pMD 18-T载体相连构建重组质粒pMDChIL-15；然后用KpnⅠ／PstⅠ双酶切下mChIL-15基因片段，定向亚克隆到经同样酶切处理的带有6个组氨酸标签的表达载体pP_RoEX~HTa中，构建重组质粒pP_RoChIL-15，对其测序确认读框正确后，转入大肠杆菌DH5α中诱导表达并进行纯化，用重组ChIL-15(rChIL-15)免疫豚鼠，制备多克隆抗体；再用HindⅢ／PstⅠ从质粒pP_RoChIL-15上切下mChIL-15基因，定向亚克隆到经同样酶切处理的杆状病毒转移载体pMelBacB中，经酶切、PCR和序列测定鉴定后，与杆状病毒线形化DNABac-N-Blue~(TM DNA共转染Sf9昆虫细胞，经三轮蚀斑纯化，构建重组杆状病毒rBac-ChIL-15，用该重组病毒感染处于对数生长期的Sf9细胞，按不同的时间收取样品，离心后对其上清和沉淀进行SDS-PAGE和Western blot分析。

Instant electric field-induced domain switching, crack initiation and propagation in a poled BaTiO3 single crystal, as well as sustained field -induced indentation crack propagation and 90°domain switching in an unpoled BaTiO3 single crystal have been investigated in situ using optical microscopy.

金相显微镜原位观察了面内极化的BaTiO3单晶在瞬时电场作用下的畴变和裂纹形核、扩展的关系以及未极化BaTiO3单晶在恒电场下Vickers压痕裂纹扩展和电致畴变的关系。

There are different types of personal loans available.

有许多不同类型的个人贷款优惠。

The above mentioned teaching model of spiral rising can be used to avoid rigidness in teaching.

上述螺旋式上升的教学模式，可以避免教学形成模式後的僵化现象。