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The expressed product was purified by metal chelate chromatography, identified by Western blot and determined for activity.

表达产物经金属螯合层析柱纯化，并进行Western blot 分析及活性检测。

Each child window has to have a child ID to be identified by COXLayoutManager.

每个子窗口必须有一个孩子的ID被COXLayoutManager确定。

Crying, in our culture, is identified with childishness

在我们的文化中，哭被看成幼稚

Crying, in our culture, is identified with childishness, with weakness and dependence.

在我们的文化中，哭等同于幼稚、软弱和不能自立。

In the least 一点 Crying, in our culture, is identified with childishness, with weakness and dependence.

译：在我们的思想文化观念当中，哭哭啼啼总是跟孩子气、懦弱、不能自立联系在一起的。

Baumrind identified three patterns of childrearing:(1) authoritative,(2) authoritarian, and (3) permissive.

Baumrind 发现有3种育儿类型：1）权威型，2）独裁型，3）放任型。

Chinaberry witches'broom, Ziziphus jujube witches'broom, Mulberry dwarf disease, JWB and Paulownia witches' broom phytoplasmas were identified by comparative studies of PCR amplify of phytoplasmal 16SrDNA、23rDNA and ribosomal protein gene, heteroduplex mobility assay, RFLP of PCR-amplified products (16S rDNA), cloning and sequence analysis of 16SrDNA and rp gene. An efficient procedure for rapid identification and differentiation of phytoplasmas was established, which could identify and differentiate phytoplasma collected from field.

通过对桑萎缩病、枣疯病、酸枣丛枝病、泡桐丛枝病和苦楝丛枝病植原体16SrDNA、23SrDNA和核糖体蛋白基因的PCR扩增、异源双链迁移率分析、16SrDNA PCR产物的限制性片段多态性分析以及植原体16SrDNA和rp基因的克隆和序列分析等比较研究，建立了一种快速确定未知植原体种类和分类地位的分子鉴别与鉴定优化程序；此程序可对田间采集的各种植物植原体样品直接进行快速鉴定和鉴别。

The phytoplasmas diseases were investigated in 30 counties and cities of 10 areas in Yunnan Province. There are 12 phytoplasma diseases, including paulownia witches-broom,chinaberry witches-broom, cactus witches-broom, christmas cactus witches-broom, orange little leaf, petunia flat stem and so on were indentified using PCR with universal primers, which amplified phytoplasma 16SrRNA gene and/or ribosomal protein genes. So the kind of phytoplasma diseases in Yunnnan Province were understood. Identified the characteristic of 16SrRNA and rp gene of phytoplasma and the kind, classification and genitic relativity of these strains using RFLP, clonging and sequence analysis (nearly 20 full-length sequences of phytoplasma 16SrRNA and/or rp gene were submitted in GenBank ); The phytoplasma strains isolated from herbage plants were conserved in storeroom. 9 articles were published in the key journals of China and 1 technique invention patent was applied. The net-page about phytoplasma disease was established. 4 graduate student were bringed up.

对云南省10个地区的30余个县市地区进行了植原体病害的调查采集工作；采用植原体16SrRNA基因通用引物（R16mF2/R16mR1及R16F2/R16R2）和/或植原体核糖体蛋白基因引物（rpF1/R1）对所采集到的病害标样总DNA进行PCR扩增，根据植原体特异扩增条带的出现，共鉴定出泡桐丛枝、苦楝丛枝、仙人掌丛枝、蟹爪兰丛枝、柑桔小叶、矮牵牛扁茎等12种植原体病害，初步明确了云南省植原体病害的种类；通过RFLP技术、克隆及测序技术获得其16SrRNA及部分核糖体蛋白基因的近全长序列（已在GenBank中共登录近20余个相关序列），通过序列比较分析，明确了上述植原体株系中这两个基因的特征并确定了云南省植原体株系的种类、分类地位及遗传相关性；对一些发生在草本植物上的植原体进行了株系保存；在国内核心期刊及一般期刊发表研究论文 9 篇，申请技术发明专利1项；初步建立了有关植原体病害的网页；培养研究生4名。

Methods: red sage root,astragalus root,white peony root,corydalis tuber,fevervine herb,dahongpao,chinese gall,cinnamon bark in this preparation were identified by tlc;the content of paeoniflorin in this preparation were determined by hplc, agilent zorbax sb 4.6×150 mm,rp-18e（ 5μm） was used. the mobile phase consisted of acetonitril-water ( 15:85 ), the velocity of flow was 1.00 ml.min-1.the detection wavelength was 230 nm.

采用tlc法鉴别处方中丹参、黄芪、白芍、延胡索、鸡屎藤、大红袍、五倍子、肉桂等药材；采用hplc法测定处方芍药苷含量，agilent zorbax sb 4.6×150 mm，rp-18e（ 5 μm ）色谱柱，流动相为乙腈-水（ 15∶85 ），流速1.00 ml.min-1，检测波长230 nm。

Chirata, and they were identified as β-sitosterol and swerchirin by spectral method. These two compounds were got firstly from the petrolelum ether fraction of S. chirata.

结论］2种化合物β-谷甾醇与1，8-二羟基-3，5二甲氧基山酮均为首次从印度獐芽菜乙醇提取物的石油醚部位分离得到。

There are two types of gold deposits in this area, namely altered rock type and quartz vein type.

本区金矿床包括两种类型：柴胡栏子金矿床为蚀变岩型金矿；莲花山和红花沟金矿床属石英脉型。

You were home and it was two weeks ago.

你回到了家里。当时是两周前。