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METHODS摘要: Molecular cloning techniques were used to construct the recombinant plasmid pLMP1p53mt containing mutant p53 gene and EB virus LMP1 gene. Linear recombinant plasmid pLMP1p53mt was transfected into mouse androgenesis of germ cells by microinjection and then survival germ cells treated were planted into fallopian tubes of artificial pregnant female mice.

方法摘要：通过分子克隆技术构建含突变型p53基因和EB病毒LMP1基因的真核表达载体pLMP1p53mt；采用显微注射法将线性化的表达载体注射至小鼠受精卵的雄性原核中，然后将注射存活的受精卵植入假孕母鼠的输卵管；取其产3 wk龄子代鼠进行PCR初选，再通过Southern杂交进一步确证；利用HE染色法观察4 mo龄转基因小鼠不同组织病理变化。

The main work and the originality points of this dissertation could be concluded as the following parts:(1) To analyze systematically the mathematical model of LES of air flow in great space, under the case of isothermal and non-isothermal, as well as the combined action of buoyancy and strain. Some basic problems are studied about the LES application into airflow simulation of great space, such as boundary conditions, spatial difference scheme and time advancing scheme, time step etc., which creates the basis for the LES application to indoor airflow simulation.(2) Both the instantaneous and time averaged flow field of great space with multiple jets under isothermal and non-isothermal cases are explored with large eddy simulation method, and also discussed the dynamic characteristics and the law of fluidflow in the great space.(3) Based on the results of LES of the great space and the Fanger thermal comfort indexes, it is brought forward the concept of the series of dynamic thermal comfort evaluating indexes, which could be divided into thermal comfort index with time averaged properties PD, PPD, PMV, thermal comfort index with instantaneous properties IPD, IPPD, IPMV, and time averaged thermal comfort indexes TAPD, TAPPD, TAPMV, and transient situations time averaged thermal comfort indexes TTAPD, TTAPPD, TTAPMV. The differences among them and the calculation methods are discussed, and the four kinds of indexes are calculated with the thermal comfort index PD as an example.(4) Based on the LES results it is discussed the hot air stratification phenomena in air-conditioned buildings in the case with air supply and return registers on the ceiling and the case on the sidewall. The fundamentals of the hot air stratification are studied and the relations of estimating hot air stratification are brought forward.(5) With the advanced apparatus such as hot wire film anemometer IFA300 and laser particle field anemoscope, corresponding model test and site measurements have been done, which are compared with the simulation results and LES is proved a very promising method in air flow simulation indoor.

本文主要工作既创新点体现在以下几个方面：(1)系统分析了等温、非等温和考虑剪切力与浮升力综合作用的高大空间大涡模拟数学模型，并研究了高大空间大涡模拟在室内气流仿真应用中的一些基本问题，如边界条件、空间离散格式和时间推进格式、时间步长选择等问题，为大涡模拟在室内气流计算中的广泛应用打下了基础；(2)首次用大涡模拟方法研究了高雷诺数下高大空间多射流在等温、非等温情况下的瞬时流场和时均流场分布，并探讨了多射流流场的动态特性和流动规律；(3)基于大涡模拟的动态仿真结果和Fanger的热舒适指标，首次系统地提出了动态热舒适评价指标体系的概念：即基于时间平均参数的热舒适指标PD、PPD、PMV；基于瞬时参数的瞬时热指标IPD、IPPD、IPMV；基于时间平均热舒适指标TAPD、TAPPD、TAPMV；以及沿行动迹线的时间平均热舒适指标TTAPD、TTAPPD、TTAPMV，并分析了四类热舒适指标的差异性和计算方法，还以PD值为例对四类指标分别进行了计算；(4)基于数十种工况下空调房间大涡模拟的结果，研究了空调建筑上送上回和侧送侧回两种情况下热分层现象，并探讨了热分层的基本规律，首次提出了避免热分层现象的判断公式；(5)在暖通空调领域，首次使用IFA300热线风速仪、激光粒子速度场仪等先进设备，完成了与大涡模拟相对应的模型试验，并把实测结果与仿真结果进行了对比，说明LES在室内气流仿真方面是一种很有前景的方法。

The act of balancing their fields through the Language of ONE and free energy systems brought up all the areas in the biology that were still in extremes; and Oa ascended temporarily into conjunctive heart failure; and Mila into aneurism and stroke patterning.

通过全一之语和自由能量系统来平衡能量场的行为培育着生物体内所有仍然处于相对内的部位； Oa 提升到临时患了心结膜症；而 Mila 患了动脉瘤和中风模式。

Feature of principal lines spatial distribution: detect the principal lines through edge detecting technology, and then divide the image of palmprint into blocks with same dimension, compute the mean and variance as feature; Feature of palmprint direction: divide the image into sub-images; compute the max angle of projection and coordinate of each sub-image, which stand for the direction and location of the line on the palm; Feature of moment invariants: compute the moment invariants of each sub-image and the whole image as feature.

掌纹主线空间分布特征：利用边缘检测技术检测出掌纹的主线，将图像分割成相同大小互不重叠的子图，提取每一个子图的均值和方差作为特征；掌纹线方向特征：将图像分成不重叠的子图，利用Radon变换求出每一个子图的最大投影角度和坐标，也就是子图内掌纹线的方向和位置，构造特征向量；掌纹不变矩特征：提取图像整体和分割后的子图的不变矩作为特征。

Aiming at the signal processing problems that exist in online fault diagnosis for train running gear, based on discussing the principle of characteristic spectrum analysis, a method of full period and uniform angle sampling is applied, which transfers the vibration signal from time domain into angular domain. The angular domain signal is transferred into corresponding characteristic spectrum using FFT. Through spectrum estimation and analysis, fault characteristic spectrum values of running gear components are acquired to distinguish their faults.

在讨论特征分析方法原理的基础上，针对机车走行部故障在线监测过程中存在的信号分析与处理问题，运用整周期等角度采样方法将时域振动信号转换为角域信号，采用FFT变换将角域信号变换为对应的特征频谱，通过谱估计、谱图分析得到机车走行部各零部件的故障特征谱值，再根据该特征谱值识别机车走行部各零部件的故障。

As I said, it is easier to gain insight into the shadow than into the anima or animus.

正如我说的，获得阴影的洞察力要比获得阿尼玛或阿尼姆斯的洞察力更容易。

You have a sexy animal magnetism that can subtly seduces the object of your desires. There is a mesmerizing way about you; when you look into the eyes of your love it is as though you are looking deep into their soul.

你有性感的动物磁性，它可以巧妙的seduces的目的，您的愿望是有mesmerizing的方式约你，当你看到的眼中，你们的爱，这是因为，虽然您在寻找深部纳入其灵魂。

After superimposition of magmatic mineralization, chert-bearing water-rich carbonates, such as dolomite, ankerite and/or calcite were turned into serpentitite-talcite; greite and pyrite into pyrrhotite.

经过岩浆叠加改造，底部的纹层状含燧石富水高镁碳酸盐岩变为蛇纹石岩；胶黄铁矿、黄铁矿大部分变为磁黄铁矿。

Paragenetic association I are composed of dissolved feldspar, quartz overgrowth and authigenetic kaolinite, This paragenetic association came into being acidic environment; Paragenetic association II include illite、albite、dissolved authigenetic quarze and kaolinite、dolomite、ankerite and ferrocalcite, the paragenetic association formed alkali environment; secondly, outcrop sandstones include paragenetic association III making up of dissolved feldspar、kaolinite、dickite and ironstone too, this paragenetic association came into being ground surface acidic environment.

成岩共生组合I由长石颗粒溶解、次生加大石英和自生高岭石组成，该组合形成于酸性成岩环境；成岩共生组合II由自生伊利石、自生钠长石、自生石英与高岭石溶解、白云石、铁白云石、铁方解石组成，该组合形成于碱性成岩环境；其次，露头砂岩还包括由长石颗粒溶解、高岭石、地开石、褐铁矿组成的第III种成岩共生组合，该组合形成于地表酸性成岩环境。

It seemed overstrung to cross the ankle-deep stream with hip-reached boots, therefore we just went into the water and waded into the middle of the stream.

我们穿着长及臀部的水靴，对于齐踝深的小溪来说似乎有点虚张声势，于是我们走到水里，淌进了小溪中央。

What was it that made you want to change sider ?

是什么使你临阵倒戈？

I don't know what he wants, but let's play along with him.

我不知道他要干什么，可我