查询词典 frap

Mobility of membrane phospholipid and receptors: membrane phospholipid was labeled with the NBD-C〓-HPC and the mobility was measured with FRAP technique, the results showed that the membrane phospholipid mobility gradually deceased with the time of ligand stimulation.

1膜磷脂和受体流动性：利用NBD-C〓-HPC荧光标记细胞膜磷脂和荧光漂白后恢复技术测量膜磷脂流动性，结果显示膜磷脂流动性随配体刺激时间增加而逐渐下降。

FRAP method measured antioxidant capacity of vitamin C (as FeSO4 equivalents μg /μg) of 4.14, reflux extraction method extracted to the untreated mung bean shells, rich green bean shell GABA treatment, selenium-rich bean shell three kinds of material dealing with anti-oxidant capacities of 0.73,0.69,0.80, three peeled mung bean products are not detected because of the content extraction.

FRAP法所测维生素C抗氧化能力(以FeSO4当量计μg/μg)为4.14，回流提取法所提取到的未处理过绿豆壳、富GABA处理绿豆壳、富硒处理绿豆壳三种物质抗氧化能力分别为0.73、0.69、0.80，三种去壳绿豆产品均因所提取含量不足以检测。

The aim of this study was to examine the possible antioxidant and antiproliferative activities of the ethanol and water extracts of four Hydrocotyle species from Taiwan. ABTS radical monocation scavenging, FRAP method, DPPH radical scavenging, reducing power method, total polyphenol content, total The results showed that the water extracts of all the samples had higher antioxidant and antiproliferative activities than the ethanol extracts.

本实验针对台湾产四种天胡荽属植物之水萃取物及乙醇萃取物进行抗氧化及抗增生活性研究，分析方法包括ABTS自由基的清除、FRAP方法、DPPH自由基的清除、还原力、总多酚类含量、总类黄酮类含量、总黄酮醇类含量以及癌细胞增生之抑制，结果显示所有样品的水萃取物之抗氧化及抗增生活性皆大於乙醇萃取物，但在抗氧化活性方面，所有受测样品活性皆小於正对照组。

After the cell growth curves was recorded, RPE cells of the 3-5th passages were utilized. 2、Three different siRNA (siRNAl,siRNA2,siRNA3) targeting against human cx43 gene and one negative control siRNA were designed and transfected into cultured human RPE cells via liposome reagent. The most effective siRNA can be determined by semi-quantitative reverse transcription PCRRT-PCR. 3、To the most effective siRNA, after transfected into human RPEs with different concentration, the cellular proliferate activities were messured by MTT colorimetry ; the percentages of RPE in different cell circle phase was assayed by FCM; the changes of phenotypical properities were observed with SCM; the protein expression of cx43 was studied through immunocytochemistry stain and Weston blot; the communication intercellular was calculated with FRAP; and the ability of recovery was assessed by using an in vitro wound healing model.4、The total proteins of siRNA1 and RPE were seperated by two-dimensional gel electrophoresis and visualized by silver staining. Proteins with significant expression alterations were selected and their peptide mass fingerprints (PMFs were obtained by matrix-assisted laser desorption/ionization time of flying mass spectrometry (MALDI-TOF-MS).The PMFs were used to search NCBInr database by Auto MS-Fit software.

实验方法：1、培养原代的人RPE细胞，经过细胞角蛋白、S-100和神经胶质原纤维酸性蛋白免疫细胞化学鉴定后，通过AO/PI染色技术确定培养细胞的存活率，描记其生长曲线，第3-5代用于以下细胞实验2、生物合成针对人cx43基因的三条小干扰RNA和一条阴性RNA通过脂质体转染RPE细胞后，通过RT-PCR的方法确定抑制效率最高的干扰片断3、将该片段以不同浓度通过阳离子脂质体转染培养的人RPE细胞后，采用MTT法观察其对细胞的增殖力的作用；通过流式细胞仪观察其对细胞周期的影响；通过扫描电镜观察其对细胞形态的影响；通过免疫细胞化学和Weston blot观察其对cx43蛋白表达的作用；采用激光共聚焦和荧光淬灭恢复技术观察荧光恢复速率平均百分率，评价其对细胞间通讯功能的影响；通过制作RPE细胞损伤模型，观察其对损伤修复能力的作用4、分离纯化转染siRNA的RPE组和正常对照组RPE细胞的全部蛋白质，应用等电聚焦电泳和SDS-PAGE双向电泳技术，银染显示分离出的蛋白质斑点，经凝胶图像分析软件对两个样本进行胶图分析，寻找差异蛋白点。

For anti-oxidant capability, it was found that the 140℃ superheated steam-heated sweet potato had the highest contents of total phenol and flavonoids, and the best scavenging ability of DPPH (1, 1-diphenyl-2-picrylhydrazyl) and FRAP (Ferric reducing ability of plasma).

在抗氧化力方面，发现不论总酚、类黄酮、清除DPPH (1， 1-diphenyl-2-picrylhydrazyl)或FRAP (Ferric reducing ability of plasma)值皆以140℃过热蒸汽加热30 min者最高。

The father that if work,mad father, love annoys, disposition is too indrawn the be short ofing that the father of Mu Ne, resign oneself to adversity causes father easily to love is broken; Communicate time effectively between father and son, Mu Zizhi to be less than a hour on average to wait a moment every week, it is the cause that causes the child to become addiction.

如工作狂的父亲、爱愤怒的父亲、性格过于内向木讷、逆来顺受的父亲都易造成父爱的缺失；父子、母子之间的有效沟通时间每周平均小于一小时等等，都是造成孩子成瘾的原因。

These are vandal proof and they come with a locking mechanism.

这是野蛮的证据，他们来到了锁机制。