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The Charon library DNA was then digested with restriction enzymes BamH Ⅰ and Hind Ⅲ, and DNA fragments of length of about 0.5-1. 5kb was subcloned into plasmid pUC 18 which was also digested by BamH Ⅰ and Hind Ⅲ and dephosphorylated by alkaline phosphatese before being ligated with Charon fragments.

抽提λCharon文库总DNA，用BamHⅠ和HindⅢ完全酶切电泳，用低熔点琼脂糖回收长约0.5-1.5kb的DNA片段；连接到pUC18上，pUC DNA先用BamHⅠ和HindⅢ酶切，后用碱性磷酸酯酶脱磷。

In this study, the cDNA fragments encoding α subunit of nicotinic acetylcholine receptor was cloned from the diamond back moth, Plutella xylostella with RT-PCR techniques. The amplified fragments are 425 nucleotides in length, from which 141 amino acids were deduced.

测序结果显示，扩增的目的基因片段包含了nAChRα亚基的三种亚型，分别命名为Pxal、Pxa2、Pxa3，都具有典型的α亚基的结构：两个相邻的半胱氨酸；形成胞外环的15个氨基酸；与烟碱和α银环蛇毒素结合有关的氨基酸残基。

Mitochondrial 16S rRNA gene and COⅠ gene fragments were amplified and sequenced from 4 wild populations of Portunus trituberculatus. Respectively, 524bp and 658bp long partial gene fragments of the 16S rRNA gene and the COⅠ gene were obtained. A high percentage of A+T base composition in the two sequences was observed, which was similar to the results of studies on drosophila, shrimp, crab and other invertebrates.

对分别采自辽东湾、莱州湾、海州湾和舟山的三疣梭子蟹4个野生群体的线粒体16S rRNA和COⅠ基因片段进行了扩增和测序，分别得到长度为524bp和658bp的片段。2段序列的碱基组成均显示较高的A+T比例（16S rRNA基因70.8%，COⅠ基因63%），这与果蝇、虾类、蟹类等无脊椎动物的16S rRNA和COⅠ基因片段研究结果相似。

Methods A retrospective analysis of refixation of fractured skull bone fragments was made. From January 2001 to November 2001, we operated on 52 patients with fractured bone fragments.

2 0 0 1年 1～ 11月行开颅手术的头部外伤患者中有 5 2例骨瓣碎裂呈多块，其中 2 4例应用颅骨钛铆钉回复固定颅骨骨折片，分析其术中操作、术后临床观察及CT检查等情况。

And different kinds of curves also reflected different composes of sedimentary fragments and conditions of sediment: the curve in the shape of normality acute kurtosis reflects the sedimentary condition of sand which were blown by wind; the curve in the shape of skewness broad kurtosis means fragments formed by river; and the curve in the shape of saddlelike kurtosis shows a relationship with the condition of deep water.

不同类型的曲线反映了不同的碎屑物源组成和不同的沉积环境：在该剖面正态尖峰代表风沙物源，偏态宽峰代表河水物源，正态尖峰和偏态宽峰的叠加代表风沙物源与河水物源的混合，鞍状宽峰可能与深水环境有关。

Results:Fractures were demonstrated definitely with transaxial imaging,SSD and MPR in all patients.The length,shape,direction and displacement of fractures,the appearance,size and space location of detached bone fragments,and dislocation of joint were displayed stereoscopically by SSD.Joint disruption and detached bone fragments within joint could be best viewed by MPR.

结果：所有病例CT横断面、SSD和MPR像均明确地显示了骨折，SSD像立体地显示了骨折线的长短、形态、走向，碎骨片的形态、大小、空间位置以及骨折的移位和关节对位状况，跨关节MPR像显示关节受累和关节腔内游离碎骨片更佳。

To obtain the tervalent fusion toxin gene, three toxin gene fragments from three species of Vibrio parahaemolyticus, Vibrio vulnificus and Vibrio mimicus were connected with the flexible linker using overlap extension PCR. The three toxin gene fragments respectively encode the mature proteins of the thermostable direct hemolysin of V. parahaemolyticus, the cytotoxin of V. vulnificus and the heat-labile hemolysin of V.

采用柔性Linker和重叠延伸PCR方法将三个毒素基因，即副溶血性弧菌的耐热型直接溶血素基因tdh、创伤弧菌的溶细胞毒素基因vvhA和拟态弧菌的热不稳定型溶血素基因vmhA的成熟蛋白基因片段进行串联，得到三联融合毒素基因tdh-vvhA-vmhA。

Chapter 3, 4 and 5 report studies on several partially folded fragments, two denatured fragments and a folded fragment, respectively

第三、四、五章分别报告了对核酸酶的部分折叠片段，变性态和一个折叠的小片段的研究。摘要如下

He saw the moon regathering itself insidiously, saw the heart of the rose interwining vigorously and blindly, calling back the scattered fragments, winning home the fragments, in a pulse and in effort of return.

他看到月亮偷偷的重新聚集起来，看到它一如玫瑰的花心一般，在摸索中有力地交织着，召唤着那些四散的碎片，脉动着努力说服它们回到家的怀抱。

Sequences were aligned by BLAST method. Results showed that 309bp fragments shared high similarity with orfl38 in Ogu CMS radish and Brassica cybrids of Ogu CMS radish, which 172 nucleotide sequences and 58 amino acids were same among them. And 689bp fragments shared high similarity (100%) with Pol orf224 in B.napus, which 677 nucleotide sequences and 225 amino acids were same among them.

同源性分析结果表明，利用orf138引物所获得的309bP大白菜mtDNA特异片段均与萝卜Ogu CMS、甘蓝型油菜Ogu CMS萝卜体细胞杂种所具有的Ogu orf138高度同源，二者有172个核苷酸完全相同，有58个氨基酸完全相同；orf224引物所获得的689bP大白菜mtDNA特异片段与甘蓝型油菜的Pol orf224高度同源，二者有677个核苷酸完全相同，有225个氨基酸完全相同，同源性均达到100%。

Since historical times,England ,where the early inhabitants were Celts, has been conquered three times .

从有历史以来，英国，在此地早期居住的是凯尔特人，已经被征服了三次。

Bluetooth OBEX File Transfer Enables the sending and receiving of files on your phone via Bluetooth.

蓝牙OBEX文件移动允许经过蓝牙传送和接受文件。。。。