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Fragments from the 239th to the 944th nucleotide of the coding regions of all actin mRNAs were amplified with two actin specific primers. Fragments from the 239th nucleotide of the coding region to the end of actin mRNAs were also amplified The electrophoresis results were compared between the macropterous and brachypterous brown planthoppers.

利用两条肌动蛋白特异性引物，扩增出了肌动蛋白基因mRNA的编码区片段，另外，将肌动蛋白特异性引物作为上游引物，将与3'锚定反转录引物部分序列相同的引物作为下游引物，扩增出了从肌动蛋白基因mRNA的编码区第239号碱基到Poly尾的这段cDNA，并在翅型之间作了比较。

Sequencing analysis and database searches indicated that there were 19 known genes and 31 unknown cDNA fragments in the sequenced 72 dot blot positive clones specific for gastrula embryos,and 52 known genes and 37 unknown cDNA fragments in the sequenced 98 dot blot positive clones specific for tail bud embryos.

测序和基因数据库比对结果表明，72个原肠期斑点杂交阳性克隆中，包括19个已知基因的cDNA片段和31个没有同源性的cDNA片段；98个尾芽期斑点杂交阳性克隆中，包括52个已知基因的cDNA片段和37个没有同源性的cDNA片段。

Methods:Twelve patients with fresh transverse fracture of patella were reviewed in the study.The hemarthrosis,blood clot,fracture fragments and soft tissues between them were cleared with arthroscopy.With the help of C-arm X-ray,the fracture was reduced with manipulation method.After the articular facet reached anatomical reduction,two Kirschners were inserted percutaneously from proximal end to distal end of fracture to fix the fragments,Two hollow screw were put along the approach of Kirschners,and tension band fixation was performed with the shape of "8".Results:All the patients were followed up averagely 12.4 months.

12例新鲜髌骨横骨折患者通过关节镜清理关节腔内的血凝块、碎骨块和骨折块之间嵌顿的软组织，直视下观察骨折复位情况以指导骨折复位固定，在C形臂X线透视下帮助骨折复位，关节镜观察关节面解剖复位后，经皮从近折端向远折端平行打入2枚克氏针固定骨折块，顺克氏针拧入2枚中空螺钉，拔除克氏针，顺螺钉空心导入钢丝，行张力带&8&字固定。

The sequence of amino acid of clone CR271 was highly homologic (61% identify and 76% similarity) to blight resistance gene Xal of rice to Xanthomonas oryzae pv. Oryzae. All other fragments showed significantly amino acidhomology to the NBS-LRR type resistance gene that have been cloned. The length of JR2 and JR3 fragments were 1321bp and 896bp respectively, which did not showed significantly homology on the level of amino acid and nucleotide acid to the sequences that have been known.

其中CR271与水稻白叶枯病抗病基因Xa1(AB002266)氨基酸同源性有61％相同，76％相似，其它片段与已克隆的NBS-LRR类抗病基因同源片段都具有较高的同源性；所获得的右翼序列JR2DNA片段长为1321bp，JR3DNA片段长为896bp，此两片段在核苷酸水平及氨基酸水平上均未找到有显著同源的序列。

Assisted by DNA2.0 and Gene2Oliga software, we optimized the codon usage and secondary structure of RNA and induced enzyme sites Cla I (237 site) and Pst I (475 site) into the gene. In the first step, fragments F1 (237 bp), F2 (238 bp) and F3 (422 bp) were separately synthesized by assembly PCR. In the second step, fragments F1, F2 and F3 were separately digested by Cla I and Pst I, and then ligated into a full length lipA gene.

首先在DNA2.0和Gene2Oliga软件辅助下对lipA基因密码子及RNA二级结构进行优化并引入Cla I(237位)和Pst I(475位)酶切位点；通过组装PCR分别合成lipA基因的各片段F1(237 bp)、F2(238 bp)和F3(422 bp)；通过该基因内的Cla I和Pst I限制性酶切位点连接成完整的全长lipA基因。

Eleven subcloned DNA fragments from the 5′- upstream region of lipA, lipC and lipF of Phanerochaete chrysosporium were assayed by using the gel mobility shift assay. The total proteins extracted from P.chrysosporium mycelia grown in Kirk medium and natural fir wood chip were used to identify the segments in these 11 DNA fragments which are controlled by some regulatory proteins.

用DNA-蛋白质体外结合实验和凝胶迁移率变动分析技术筛选黄孢原毛平革菌木质素过氧化物酶基因lipA、lipC、lipF的5′-端调控区内能与该菌在木质素降解条件下形成的蛋白特异结合的顺式作用元件。

In this study, the exon2 fragments of CHS genes in ferns, gymnosperms and angiosperms, a total of 22 fragments from 12 systematic families, were amplified successfully by PCR, and then sequenced (PCR amplification of the exon 2 from musci is in process).

本实验用PCR方法从蕨类，裸子植物和被子植物，共12个科中均成功地扩增了22个CHS基因外显子2片段（从藓类中扩增CHS基因外显子2的工作正在进行）。

We found a clear trnK5'-matK-trnK3' structure in Ophioglossum petiolatum, Lycopodiella cernua and Selaginella doederleinii. PCR with degenerate primers failed to amplify any trnK or matK fragments from other ferns or allies. However, dot blot hybridization showed distinct signals in these plant failed to amplify matK fragments.

本论文鉴定原始陆生植物其他未被检视的重要类群中叶绿体matK基因的存在，结果发现瓶尔小草、过山龙、生根卷柏都有trnK5'-matK-trnK3'的结构，以RT-PCR侦测matK表现时，瓶尔小草和过山龙都有侦测到讯号，但生根卷柏却没有，显示matK的表现在原始陆生植物似乎不是那麼一致。

The papyrus fragments were discovered in historic dumps outside the Graeco-Egyptian town of Oxyrhynchus ("city of the sharp-nosed fish") in central Egypt at the end of the 19th century. Running to 400,000 fragments, stored in 800 boxes at Oxford's Sackler Library, it is the biggest hoard of classical manuscripts in the world.

19世纪末，人们在埃及中部的希腊化的埃及小镇奥斯莱卡（Oxyrhynchus，含意是尖鼻子鱼城）外的历史废墟中发现了这些浦草纸古卷残片，最后残片的总数高达400，000片，保存在牛津大学萨克勒图书馆，这是世界上最大数量的古典文献手稿。

Numerous fragments of his thirty-seven volume treatise On Nature have been found among the charred papyrus fragments at the Villa of the Papyri at Herculaneum.

他的三十七卷著述《论自然》的大量片断在赫库兰尼姆古城的帕比里庄园的烧焦草纸残片中找到了。

Since historical times,England ,where the early inhabitants were Celts, has been conquered three times .

从有历史以来，英国，在此地早期居住的是凯尔特人，已经被征服了三次。

Bluetooth OBEX File Transfer Enables the sending and receiving of files on your phone via Bluetooth.

蓝牙OBEX文件移动允许经过蓝牙传送和接受文件。。。。