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After induction and massive expression of the 3 fusion proteins, the soluble fraction was purified with 50% Ni-NTA resin affinity chromatography, which was proven by SDS-PAGE of the eluate to be a method that can achieve high purity of fusion proteins.

大量诱导表达三种融合蛋白后，取含FA、FB融合蛋白的可溶性组分用50％Ni-NTA树脂过柱纯化，将洗涤部分进行SDS-PAGE检测，表明用此纯化方法能纯化到高纯度的融合蛋白。

The crude extracts were isolated by column chromatography of alkaline alumina, and a bioactive fraction was obtained from the 80%ethanol eluate.

此液经碱性氧化铝柱层析分离，其80%乙醇洗脱的 C 组分显示生物活性。

he fraction of pneumonia that tends to result in empyema is still low, so there is a huge benefit from immunization," Dr. Tancredi said in a press release.

Tancredi医师在一篇新闻稿中表示，倾向造成肺脓疡的肺炎比例仍然偏低，因此接种疫苗所带来的好处仍然是非常大的。

Different fraction has different concentration REE, and the REE original value of eolian may be affected by wind choice.

且黄土一古土壤的REE含量可能受风力分选的影响。

EPACT requires that a fraction of new purchases of light-duty vehicles 41 for qualified fleets be alternative-fuel vehicles.

能源政策法规定，一个新的采购光部分的合格船队轻型车辆41替代燃料汽车

In a global context, moreover, it is but a small fraction of the larger failure of the United States to deal equitably with the human fallout from its national security policies.

在全球范围内，此外，也只不过是一小部分规模较大的失败，美国处理公平与人权的微尘，其国家安全政策。

About 2.6%~27.1% high graders appeared the situations that suffer denominator or numerator control, while handling the problem of equivalence fraction of continuous quantity or discrete quantity.

在等值分数概念表现上，约有2.6﹪~27.1﹪的学童在处理连续量或离散量等值分数问题时，均出现受分母或分子控制的情形，用以判断分数值所表示的具体量。

Results There were significant differences between MDS and CAA in Hb, red cell distribution width-coefficient variation, immature reticulocyte fraction, BPC, the ratio of G1 (the sum percentage of myeloblast and premyelocyte) to G2 (the sum percentage of neutrophilic myelocyte and metamy-elocyte), the ratio of E1 (the sum percentage of proerythroblast and early erythroblast) to E2 (the sum percentage of intermediate erythroblast and late erythroblast), megakaryocyte count,erythroblast PAS, neutrophil alkaline phosphatase, and serous levels of indirect bilirubin,lactose dehydrogenase, folic acid, VitBl2 and ferritin.

结果 MDS患者血红蛋白，红细胞体积分布宽度。变异系数、未成熟网织细胞比率、血小板计数、骨髓原始细胞及早幼粒细胞之和与中性中幼粒细胞及中性晚幼粒细胞之和的比值、原始红细胞及早幼红细胞之和与中幼红细胞及晚幼红细胞之和的比值、巨核细胞计数、有核红细胞糖原染色阳性率和阳性指数、中性粒细胞碱性磷酸酶染色阳性率和阳性指数、血清间接胆红素、乳酸脱氢酶、尿酸、叶酸、维生素B12(VitB12)、铁蛋白水平等常规实验室指标与CAA患者比较差异有统计学意义。

A new technology, Supercritical Fluid CO_2 Extraction, was also supplied to study the plant. The technological parameter was optimal. Total extract of SFE-CO_2 were 4.86 times of the essential oil extracted by SDE, main active extracted constituents were 1.36% high than SDE. The chemical constituents of SFE-CO_2 were also analyzed by GC-MS. 57 constituents were identified, 29 constituents of them were first discovered. 24 constituents were discovered in two extract. Fingerprint is a good technology to elevate the quality of herb medicine. The main active fraction can be extracted by SDE and SFE, Fingerprint was studied respective, chromatographic parameter was acquired. 22 peaks were assigned common peaks in the spectrum of essential oil, 17 peaks were assigned to common peaks in the spectrum of SFE extract.

采用现代共性关键技术超临界CO_2萃取技术，通过正交试验，优选了超临界CO_2提取牛至挥发油的工艺，并与传统的水蒸气蒸馏法进行了比较研究，结果显示：前者提取有效部位的收率为后者的4.87倍，主要有效成分的转移率是后者的1.36倍；采用GC-MS分析方法和NIST质谱库检索，对SFE和SDE所提化学成分进行比较，SFE分离鉴定了57种化合物，占提取物总含量的86.96％，其中29种化合物为首次从该植物中鉴定；后者分离鉴定了69个化合物，占提取物总量的98.28％，其中27种化合物为首次从该植物中鉴定；两种方法的提取物中共有成分25种，包括主要有效成分麝香草酚、香荆芥酚、对聚伞花素等。

The estimative reliability model involving the residual life of the high temperature boiler pipe of steam-generator has been established by using of reliability theory and fraction criterion of life loss.

利用可靠性理论和寿命损耗分数准则，建立了蒸汽发生器高温炉管的剩余寿命可靠性预测模型，并应用实例对不同模型进行了对比分析，确定了蒸汽发生器高温炉管剩余寿命可靠性预测的准确方法，可为蒸汽发生器高温炉管的管理和使用提供参考依据。

For every one of my New Year resolutions, I have been a backslider for years.

说到我的新年决心，我每年都做

With empathy and focus on a feeling level, we can all relate to the common ground of mutuality of emotions.

有了同情心和感觉上一级的重点，我们都可以涉及到相互的情感共同点。