查询词典 flic

The 528 bp fragment of flic gene was successfully cloned into vector, the pGEX-flic recombinant plasmid was expressed and induced by IPTG and Western-blot method showed good antigenicity of the recombinant protein, and there were flexible regions such as coil region and turn region in flic protein.

获得的目的基因片段长度为528 bp，测序表明其序列与GenBank公布的一致；软件预测结果显示，该蛋白具有多个明显的二级结构成分及跨膜区；SDS-PAGE检测表明，在约50 ku处有一特异性条带；Western-blot检测表明，表达蛋白具有良好的抗原活性。

The study cloned the flic gene of App and performed sequencing and analysis of biological information. Polymerase chain reaction PCR was used to amplify the flic gene from.

构建猪传染性胸膜肺炎放线杆菌(Actionobacillus pleuropneumoniae， App)鞭毛蛋白编码基因的重组表达质粒，对其编码蛋白进行生物信息学分析，并将其在原核细胞中进行表达。

The flic gene of App was obtained by PCR based on highly homology with Escherichia coli, Salmonella, Shigellosis. Then it was legated to pMD-18T vector. The recombinant plasmid was identified by enzyme and sequence analysis. The 528 bp fragment of flic gene was successfully cloned into pGEX-KG vector, then the recombinant plasmid was transformed into E.

以猪传染性胸膜肺炎放线杆菌血清Ⅰ型菌株基因组为模板，根据其鞭毛区与其它细菌高度同源性设计引物，利用PCR方法扩增鞭毛蛋白基因flic片段，将其亚克隆到pMD-18T载体中并进行PCR和酶切鉴定，再将其亚克隆与载体pGEx-kG分别双酶切和连接，构建重组表达载体pGEX-flic，并将其转化大肠杆菌工程菌BL21，进行原核表达。

These results provided reference for research on antigenicity and other function of flic of bacteria.

为进一步研究细菌鞭毛的抗原性及其他功能提供参考。

You can also output the video to FLIC animation file.

您也可以输出视频flic动画文件。

You will want to repeat the components of this trick to call it a proper Flic Flac.

你需要重复这个部分，才能使这个技巧成为一个合格的Flic Flac。

Objective To study the possibility of measuring quality of life in patients with liver cancer by FLIC scale.

目的 探讨FLIC量表用于测定肝癌患者生命质量的效果。

Tip: form this fade position, you can now go into flic flacs and more….

提示：从这个飘姿，你就可以接FlicFlac和更多

Results The QOL-LC is of better validity, reliability and responsiveness; the FLIC is of good validity, reliability and better responsiveness.

结果 QOL-LC的效度、信度和反应度均较好；FLIC的效度、信度尚可，反应度较好。

A confirmed flic gene has been obtained and analyzed, providing a good foundation for research on motility and ontogenesis of App furtherly.

成功获得了App的flic基因，其编码的蛋白质具有较多的α螺旋区和无规则卷曲区域，表达的鞭毛蛋白具有生物学活性。

"Second Life is remarkably easy to work with, and is very popular,"

"第二次生命是显着容易的工作，并且很受欢迎，"

For example, we usually assume that materials are homogeneous and isotropic and free of internal defects or flaws.

为了得到适合有限元分析的模型，我们必须经过如图2所示的简化步骤。