查询词典 flic

The secondary structure and membrane-spanning regions of flic protein was predicted by biosoftware.

利用PCR方法扩增flic基因片段，将其克隆至pMD-18T载体后测序，利用生物信息学软件对其编码蛋白的二级结构及跨膜区进行预测。

A multiplex PCR assay for detection of Escherichia coli O157:H7 was developed by using 3 sets of primers that specifically amplify segments of the rfbE、fliC and eaeA genes. The target genes fragment of the PCR assay were 291 bp, 625 bp and 368 bp, respectively. Analysis of 30 strains demonstrated that this PCR system was specific.

以编码大肠杆菌O157抗原的rfbE基因、编码H7抗原的fliC基因以及编码毒力因子的eaeA基因为靶基因，选择3对引物，建立并优化了检测大肠杆菌O157：H7的多重PCR体系，扩增产物分别为291 bp、625 bp、368 bp，采用30株细菌验证了该多重PCR具有特异性。

In the state large-and-middle scale coal enterprise, because the technology mining under flic building is not ripe enough, the coal pressed under the village restricts the coal enterprises development seriously.

在国有大中型煤炭企业中，由于建筑物下开采技术不够成熟，村庄下压煤严重制约着煤炭企业的发展。

2The scale had good content validity. Its construct was consistent with the concept designed. The factor loading spectrum and ANOVA are 0.63-0.79 and 7.44, 0.67-0.76 and 5.42, 0.69-0.88 and 4.77, 0.66-0.91 and 4.77, 0.63-0.80 and 4.38 respectively. The criterion validity with HAD and FLIC were 0.78 and 0.69 respectively.

2问卷具有较好的内容效度，结构与设计的理论构思相符，5个因子负荷范围和方差分析分别为0.63~0.79和7.44，0.67~0.76和5.42，0.69~0.88和4.77，0.66~0.91和4.77，0.63~0.80和4.38，参照于HAD和FLIC两个问卷的效标效度分别为0.78和0.69。

I had a lovely dog named PiPi, he was one of my best friends.

我曾经有过一只小狗，它的名字叫皮皮，它很善良，它是我最忠实的朋友。

Newly ovulated mouse oocytes were cultured in the CZB medium supplemented with different concentrations of pyruvate before treated for activation or assayed for MPF activity.

我们猜测卵母细胞在不含丙酮酸的条件下老化的更快，并且卵丘细胞可能通过消耗不含葡萄糖的CZB中的丙酮酸从而促进卵母细胞老化。