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When the volumetric ratio of medium to flask was 0.20 and the corn steep liqud concentration was 8g/L, there have high yield of pyruvate and lactate produced at growth phase, 4.1g/L and 1.0g/L, respectively, which are ...

在装液比为 0 2和玉米浆浓度为 8g L时，丙酮酸和乳酸在细胞生长期可分别积累达 4 1g L和 1 0g L ，比正常发酵时增加 2倍以上，丙三醇产率也低；然而，装液比为 0 0 8和玉米浆浓度为 4g L时，丙酮酸和乳酸产生较低，丙三醇产率较高，但乙酸积累比供氧不足时高，可达 2 6g L。

The volumetric flask is then filled with a stock solution consisting of 0.010 M HNO3 and 0.001% disodium EDTA dihydrate provided by the TA.

然后用助教提供的，含有0.010M HNO3和0.001%二水合乙二胺四乙酸二钠的储备液，注满容量瓶。

Sodium chloride standard solution Dissolve 0.165 g of sodium chloride in water in a 1000 ml volumetric flask, and dilute to the volume, mix well.

标准氯化钠溶液的制备：称取氯化钠0.165g，置1000ml量瓶中，加水适量使溶解并稀释至刻度，摇匀，作为贮备液。

MERCURY STOCK SOLUTION : Transfer 135.4 mg of mercuric chloride to a 100-mL volumetric flask, and dilute with 1 N sulfuric acid to volume.

MERCURY储备溶液：升汞调动135.4毫克到100ml容量瓶， and稀释与1 N硫酸对容量。

Cool the solution, cautiously dilute with water, transfer to a 200-mL volumetric flask, dilute with water to volume, mix, and filter.

冷却解答，慎重地稀释用水，调动到200ml volumetric烧瓶，稀释用水对容量、混合和过滤器。

Through shake flask fermentation, the effects of microwave radiation, the exogenous gentamicin concentration, concentration of NaCl and some other factors on the secretion of gentamicin were studied.

研究了外加抗生素、胞外庆大霉素浓度、微波处理、培养基中氯化钠浓度等因素对于细菌庆大霉素分泌的影响。

Through shake flask experiments,it was acquired that the best culture conditions:pH6.5,37℃,240rpm,40mL/300mL,and 2.5%of bacterium culture for 12h.

通过摇瓶发酵试验，确定其最佳培养条件为：摇床37℃，240rpm，发酵培养基的起始pH为6.5，接种量为2.5%，装液量为40mL/300mL，培养32h。

One L-isoleucine high-producing strain SW0370 was screened through protoplast fusion, shake flask compound screening, genetic marker test and single clone isolation and continuous passage cultivation.

3确定了亲株ISW330和AS1.495原生质体形成和再生及进行原生质体融合的最佳条件，采用原生质体融合技术最终筛选出一株带有目的遗传标记的L-异亮氨酸高产菌SW0370〓。

2The protoplast formation and regeneration and fusion conditions of strain ISW330 and AS 1.495 were studied in this thesis. One L-isoleucine high-producing strain SW0370 was screened through protoplast fusion, shake flask screening, genetic marker test and single clone isolation and continuous cultivation for generations.

2确定了亲株ISW330和AS1.495原生质体形成和再生及进行原生质体融合的最佳条件，采用原生质体融合技术最终筛选出一株带有目的遗传标记的L-异亮氨酸高产菌SW0370(Leu~-+Met~-+AEC~r+á-AB~r)。

One L-isoleucine high-producing strain ISW330 with genetic marker was screened through shake tube primary screening, shake flask compound screening, genetic marker test and single clone isolation and continuous passage cultivation This strain could produce L-isoleucine 9.18g/L after fermentation under the fermentation condition without being optimized.

以黄色短杆菌HL41为出发菌株，通过硫酸二乙酯和紫外线诱变处理，经摇管初筛、摇瓶复筛、遗传标记验证、单菌落分离和连续传代，筛选出一株L-异亮氨酸生产菌株ISW330(Met~-+AEC~r+α-AB~r)。

Must have Coordination and negotiation skill, flexible ideas, and be punctilious person.

必须具备较好的协调与谈判能力，头脑灵活，工作认真仔细

Result: 18 patients are all with simple eye.The pigment mole lies in nasal and temple sides of the ball conjunctiva,in which 2 lie in lacrimal caruncle and semilunar plica.

结果：18例患者均为单眼，色素痣大多数位于内外侧睑裂部球结膜面，2例位于泪阜及半月皱襞，经激光治疗后，10例色素痣消失，有5例行再次激光后消失，2例行第三次激光后消失，1例第四次激光后消失。