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100ML culture flask, nitinol wire, cardiac pacemaker and connecting lead were used to make electric field.

使用一次性100mL培养瓶、镍钛合金金属丝、心脏起搏器和连接导线设计制作电场。

Under the optimum concentration of 1.5% glycerol in fermentation medium and feeding of 1% glycerol as precursor after 60 h of incubation, the titer of 2,668 μg/ml was obtained in shake flask culture, an increase of 7.4 times compared with the original strain.

以XC-5-48菌株为试验菌株，当培养基中甘油初始浓度为1.5%，并且在发酵60h时补加1.0%的甘油，摇瓶发酵的效价可达2668μg/ml，较出发菌株(318μg/ml)提高了7.4倍。

Results The open cycling air pressure control culture system worked effectively, the pressure inside the culture flask can be controlled from 0 to 100 mm Hg.

结果成功组装开放式压力控制培养系统，通过调节进出气流速度，可将培养瓶内压力控制在0～100 mm Hg（1 mm Hg=0.133kPa）之间。

The hyaluronic acid production by batch and fed batch culture s in flask and fermentor was studied, and the primary mechanism was discussed.

在耗糖量相同的情况下，分批发酵比多次加料或流加发酵具有更高的HA产量和转化率；分批发酵初糖7％，发酵24h 左右，产HA3。

Once the microsphere was fractured and in contact with the surface of culture flask, cells spread and attached onto the plastic substratum and proliferated.

当球体破裂而接触培养皿表面时，细胞会在塑胶基质上有吸附与伸展的现象并且开始增生。

Gentamicin sulfate in the production process in the narrative We know that gentamicin sulfate using fermentation technology training, divided into training and seed bottle fermentation flask culture.

在对硫酸庆大霉素生产工艺流程的叙述中，我们知道硫酸庆大霉素采用摇瓶发酵培养工艺，分为种子瓶培养和发酵瓶培养。

After further purification by a SuperdexTM 75 HR 10/30 column, finally 6-7 mg of Cd2+-hMT-3 was obtained from 3 L of flask culture with the recovery of about 1.8%.

从摇瓶培养菌液中纯化获得了结合有Cd2+的完整的人金属硫蛋白-3，得率为1.8%。

Third passage osteoblasts were plated at the density of 5×104/ml in 75ml culture flask, twenty four hours later treatment factors were added. Total RAN was extracted from osteoblasts using guanidine, and mRNA expression of OPG and RANKL were examined using RT-PCR.

OPG作为RANKL的诱骗受体阻断其与表达于破骨细胞前体细胞和成熟的破骨细胞表面的功能性受体RANK的作用，从而抑制破骨细胞的分化和功能。

The third passage osteoblasts were plated at the density of 5×104/ml in 75ml culture flask, twenty four hours later treatment factors were added. Total RAN was extracted from osteoblasts using guanidine, and mRNA expression of OPG and RANKL were examined using RT-PCR.

OPG作为RANKL的诱骗受体阻断其与表达于破骨细胞前体细胞和成熟的破骨细胞表面的功能性受体RANK的作用，从而抑制破骨细胞的分化和功能。

The third passage osteoblasts were plated at the density of 5×104/ml in 75ml culture flask, twenty four hours later treatment factors were added.

许多激素及细胞因子通过调节破骨细胞和成骨细胞这两类细胞的数量和活性，使骨量维持动态平衡。

The changes of the HAF after liver injury may be related to the volume of blood loss, the intraabdominal pressure and the pneumoperitoneum duration.

肝撞击伤伴失血后肝动脉血流量的变化与失血量、气腹压力以及气腹持续时间等有密切联系。

Reneging on our commitments to the world's poor cannot be an austerity measure, she said.

放弃我们给世界上的穷国的承诺不能成为一种紧缩措施。