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Subsequently, BMSCs were incubated in serum-free L-DMEM, and placed in a plastic culture flask at 1.0×108/L.

按1.0×108 L-1的密度接种到一次性塑料培养瓶中进行培养。

Results obtained included:(1) MSCs began to attach to culture flask after 24h cell inoculation by centrifugation method.

5观察经Ⅰ型胶原包被的多孔块型PLGA材料体内成骨效果，尤其是材料中心部位的成骨情况。

In current thesis, various substratess, which influenced the biosythesis of GOD in resting cell culture system and shake flask has been studied.

本文以葡萄糖氧化酶为研究对象，通过建立静息细胞培养系统及膜透析发酵系统，考察了影响GOD生物合成的因素，并对GOD产生菌的发酵动力学进行较详细的研究。

The story goes (De Kruif, 1953) that Pasteur's research was interrupted by a holiday and a culture was left in a flask in the laboratory.

事情是这样的（ De Kruif， 1953），巴斯德因一个假期而中断了他的试验，并将一个细菌培养物留在了实验室的细颈烧瓶中。

108 Units HuIFNγ tas been produced from l liter of bacteria culture in flask.

SDS-聚丙烯酰胺凝胶电泳后的扫描表明，γ干扰素的表达量约占细菌可溶性蛋白总量的30％。

The fermentation culture medium and shaking flask fermentation conditions of glutathione have been researched by using orthogonal experiments with Saccharomyces cerevisiae.

通过正交试验，研究了啤酒酵母摇瓶发酵生产谷胱甘肽的培养基的组成以及发酵培养条件。

The effect of nitrogen source, initial pH, quantity of incubated in flask, culture temperature and time on producing chitinase of Pseudoalteromonas H7 were studied systematically in this paper.

系统地研究了氮源、起始pH、培养基装量、培养温度和时间等因素对交替假单胞菌H7产甲壳素酶的影响。

After repeated short time digestion of pancreatic tissues with collagenase Ⅴ, cultured cells were transferred to a new culture flask.

用胶原酶反复短时间消化胰腺组织，在细胞接种后及时转板的方法可获得生长良好的高纯度胰岛原代单层细胞，适合用于实验研究。

Therefore, the biomass concentration during flask culture can be predicted by this neural network model if initial glucose concentration and initial biomass concentration were provided.

所以，由培养液的初始葡萄浓度和初始生物量浓度通过网络软测量模型就可对摇瓶中微生物的生物量浓度进行估算。

High level of Kal was obtained in BMMY medium (pH 7.0) after 96 hours induction of 29?C and 2% methanol, with the highest yield of 14 mg/L in shake flask culture.

阳性表达菌株在BMMY培养基(pH 7.0)中29℃培养，经2％甲醇诱导表达96 h，摇瓶表达量可达14 mg/L。

Yet, Edith says, there was always money in the bank.

但是Edith说，她的银行户口里面总是有钱。

We have created a series of files that resolve this problem--one for each day of the 40 days of purpose.

我们制作一系列的档案去解决这问题---一个为标竿40天中的每一天。