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The experimental study is to explore the effect about rhEPO on neuromotor function, secondary pathology change and expression of NTF and the apoptosis of neural cell, which administered at different time after traumatic spinal cord injury. The experimental study is devided into three parts: The first part The effect of rhEPO on NT-3 and NGF expression after traumatic spinalcord injury Objective Nerve growth factor and neurotrophic factor–3(NT-3) are chief members in neurotrophic factors family, which can promote nerve cell survival、growth and differentiation.

本研究拟在脊髓损伤后不同时间应用促红细胞生成素干预，探讨其对神经运动功能和继发性病理改变的保护作用及对神经营养因子表达和神经细胞凋亡的影响，共分为以下三个部分：第一部分脊髓损伤后促红细胞生成素对NGF、NT-3表达的影响目的：神经生长因子和神经营养因子-3(NT-3)是神经营养因子家族的主要成员，是机体产生的能够促进神经细胞存活、生长、分化的一类多肽或蛋白质因子。

We examined 2 representative growth factors: brain-derived neurotrophic factor and vascular endothelial growth factor.

检测两种典型的生长因子：脑源性神经营养因子和血管内皮生长因子。

Vascular endothelial growth factor (VEGF or VEGF-A) is a hypoxia induced angiogenic growth factor that is potent in neurotrophy, neuroprotection, anti-apoptosis and cell proliferation.

VEGF是一种由缺氧诱导的血管生长相关肽，在脑内有比较丰富的表达。VEGF具有神经营养、神经保护、抗凋亡、细胞增殖、提高毛细血管渗透压等广泛的生物学作用。

Results: Fujian Tablet could increase the expression of neurotropic factor and decrease that of inhibitory factor. It promoted proliferation, migration, differentiation of neural stem cells, axonal growth and synapse formation remarkably.

结果：复健片可增加神经生长营养因子表达，减少抑制因子表达，对神经元增殖、迁移、分化，轴突延伸、突触形成等有明显促进作用。

Nerve grows factor and brain-derived neurotropic factor, as one of the important active molecules, belong to neurotrophic family, which can affect the development of neuron and prevent neuron from damage.

神经生长因子（Nerve grows factor，NGF）和脑源性神经生长因子(Brain-derived neurotropic factor，BDNF)属于神经营养素家族，是神经系统重要的活性分子之一，是中枢神经系统胆碱能神经元、周围神经系统交感神经元及感觉神经元发育、分化、功能活动必不可少的营养分子。

Methods: Based on the theory of "kidney produces sui", we set up MCAO rat model. Five indexes including Nestin, PSA-NCAM, MAP-2, GAP-43 and SYN38 were measured in situ hybridization and immunohistochemical methods to evaluate the effects of Fujian Tablet on the proliferation, migration, differentiation of neural stem cells, axonal growth and synapse formation. Other two indexes including trk-B, NOGO-A were used to assess the effects on the neurotropic factor and inhibitory factor.

以&肾生髓&的理论为依据，以MCAO大鼠为实验对象，利用原位杂交技术和免疫组织化学方法，分别以nestin、PSA-NCAM、MAP-2、GAP-43、突触素p38为指标，观察复健片对神经干细胞增殖、迁移、分化、轴突生长、突触形成的影响；分别以trk-B、NOGO-A为指标，观察复健片对神经生长营养因子、抑制因子的影响。

Part 4: Brain-derived neurotropic factor genetically modified olfactory ensheathing cells of the neonatal rat Objective To culture the olfactory ensheathing cells from the neonatal rat and modify them genetically by human Brain-derived neurotropic factor to increase the formation of neurophic factors. Methods The OECs were dissociated from the neonatal rat olfactory bulbs and purified with time-speed difference stick.

第四部分：脑源性神经营养因子基因转染新生大鼠嗅鞘细胞目的为提高嗅鞘细胞(Olfactory ensheathing cells，OECs)分泌脑源性神经营养因子(BDNF，Brain-derived neurotropic factor)的能力，探讨将外源性BDNF基因转染入OECs，为今后采用BDNF基因修饰的OECs移植治疗SCI奠定基础。

The current methods of studing the mechanism of asthma aboard is take the nonasthmatic ASMCs as object and study the effect of every single related factor on the on proliferation of nonasthmatic ASMCs in vitro respectively. However, as the phenotypes and functional status of asthmatic and nonasthmatic ASMCs are different, it's deficient to infer the proliferative feature and the signal transduction pathway of asthmatic ASMCs in vivo from that of nonasthmatic ASMCs induced by every single factor in vitro.

目前国内外通常是分别研究与哮喘有关的各种影响因子对非哮喘ASMC增殖的影响，但由于非哮喘ASMC和哮喘ASMC的表型和功能状态存在差异，这种在体外分别以单一因子诱导非哮喘ASMC增殖来推断哮喘ASMC体内的增殖特性及信号转导途径的方法尚存在一定局限性。

In this thesis, several problems of time-dependent systems in various branches of physics are investigated by making use of the generalized invariant theory and the invariant-related unitary transformation formulation. The problems are given as follows:(1) Spin model and geometric phase factor in the fiber We investigate the fiber experiment performed by Chiao-Tomita and the Chiao-Wu theory concerning the geometric phase factor in the optical fiber, and then show that the investigation of the propagation of photons inside the noncoplanar optical fiber can be regarded as that of a second-quantization spin model.

本论文用推广的不变量理论和与不变量有关的幺正变换方法研究了一些物理分支中的几个含时系统问题：(1)自旋模型与光纤中光子几何相因子问题重新考察了Chiao-Tomita光纤实验和Chiao-Wu关于光纤中光子几何相因子的理论，证明了在非共面弯曲光纤中传播的光子运动问题实际上可以化为二次量子化含时自旋模型研究。

The third chapter talked about two different treatments of quantum decoherence in V〓 system. For one the decoherence factor is been expressed in the form of a random external field. And the mean density matrix is calculated to signal the decoherence by showing its nondiagonal elements to approach to zero. In another numerical work done by us, random interaction between system and environment is imposed to mimic the decoherence factor.

第三章讨论了V〓体系中量子相干性研究的两个工作，一个用随机外场来代表消相干因素，采用对系统的密度矩阵进行随机平均的解析方法得到一些结果，一个是我们用数值计算方法的处理，其中消相干因素考虑成系统和环境的相互作用。

Don not attempt to do something which you can not to do.

不要企图做那些办不到的事情。

The expression of CTGF and TNF-αweredetected by immunochemistry and the number of Clara Cells was calculated.

光镜下观察肺组织的病理变化，采用免疫组化染色观察肺组织中结缔组织生长因子和肿瘤坏死因子-α的表达和Clara细胞的数量。