查询词典 explicit identification

Pneumophila serogroup 5 antibody to develop two immuo-colloidal gold tests respectively. Main works listed below as:1、Preparation and identification of polysaccharide antigen of L. pneumophila Bacteria of LP1 ~ 7,9 and 10 were cultured on yeast agar buffer activated carbon for three to five days at 37℃, 5%CO2. Protein-free polysaccharide antigens were obtained after harvest in cells, extraction, deproteinization, dialysis and other steps. Their immunogenicities were verified by ultraviolet spectrophotometer full-wavelength scanning and Western blotting.2、Preparation and identification of rabbit anti-LP1 antibodies and rabbit anti-LP5 antibody Rabbit anti-LP1 and anti-LP5 antibodies were purified after rabbits were immuned with antigens isolated as described above. The purities of both antibodies were above 80% and the titer of blood serum 1:32 tested by double antibody sandwich assay.3、Development of colloidal gold immunochromatographic assay kit The size of colloidal gold particles in the kit was 25nm. The optimal concentrations for antibodies were 30μg/ml and the sensitized concentrations of NC membrane were 5 mg/ml.

主要研究工作从以下几个方面进行：1、LP1~7、9和10型多糖抗原的制备与鉴定将LP1~7、9和10型菌株分别接种在缓冲活性炭酵母琼脂培养基上，37℃、5%CO2的条件下培养，3~5天后洗下菌苔，经抽提、除蛋白、透析等步骤后得到基本无蛋白的LP多糖抗原，经紫外分光光度仪全波长扫描及Western blotting验证其抗原良好。2、兔抗LP1抗体和兔抗LP5抗体的制备与鉴定分别用LP1、LP5型多糖抗原免疫家兔，采用琼脂糖双向扩散试验检测，两种抗体血清效价均为1：32；饱和硫酸铵法提取抗体，SDS-PAGE检定其抗体纯度均达到80%以上。3、胶体金免疫层析检测试剂的初步研制采用柠檬酸三钠还原法制备约25nm大小的胶体金颗粒；分别制备兔抗LP1抗体、兔抗LP5抗体的金标探针，两种抗体的最适标记量均为30μg/ml；选择适当孔径的微孔滤膜为载体包被两种抗体，NC膜包被浓度均为5mg/ml。

Key words: Radio Frequency Identification technology smart labels because a large quantity of data storage, read and write speed, data safe, easy to use, such as reading and writing distance notable features are being rapidly recognized and widely used for identification, and special management, materials tracking, security, traffic, animal management, and other fields.

射频识别技术的智能标签因其数据存储量大、读写速度快、数据安全性高、使用方便、读写距离远等显著的特点，正得到快速的认可，并广泛应用于身份识别、物流管理、物品追踪、防伪、交通、动物管理等领域。

Flow regime and void fraction are important parameter in gas-liquid two-phase flow, the relation of differential pressure signal coming from Venturi tube and the flow regime of gas-liquid two-phase flow was studied in this paper, A kind of flow regime identification method of gas-liquid two-phase flow based on Hilbert-Huang Transform and fuzzy inference was put forward, the familiar flow regime identification of gas-oil and gas-water two-phase flow was achieved.

两相流动是一个十分复杂的过程，参数较多，检测相当困难。流型、空隙率是气液两相流中非常重要的参数，而且测量比较困难，还影响两相流其它参数的测量。

Corn hybrid variety identification hasalways been a difficult point of seed testing in china,it has important practicalsignificance to study and spread fast corn variety identification for country andenterprises.

而玉米杂交种纯度鉴定是我国种子检验工作的一个难点，研究和推广快速玉米种子纯度检测技术，对国家和企业具有极其重要的现实意义。

At the same time, the study found an easy and simple resistant identification method in seedling stage and carried on resistant identification of Verticillium dahiea wilt to tomato germplasm resources that are kept by our lab.

生物学特性研究表明，适于番茄黄萎病菌生长的培养基为PSA培养基，低量蔗糖酵母培养基，马铃薯胡萝卜培养基及蔡氏培养基；在马铃薯胡萝卜培养基上产孢效果最理想。

We made all-round serological tests to the two RhD-- individuals,including identification of blood group、Coombs test、antibody screening and identification、antibody titer and HDN test,together with investigation of genealogical blood group to all family members by classic hemagglutination methods.

我们采用经典的血细胞凝集法，对2个体进行了血型血清学检测，包括血型鉴定、抗人球蛋白试验、抗体筛选与鉴定、抗体效价检测与新生儿溶血病检测，并对两个家系所有家系成员进行家系血型调查。

After pathogenic fungi identified,we found 26 of them can cause cotton leaf spot. The 26 pathogenetic strains were carried out morphological and molecular identification of the firm. The initial identification of the pathogens is through the morphological observation such as the determination of growth, colony morphology, conidia and conidial stalk.

通过对生长量的测定，菌落形态，分生孢子和分生孢子梗等形态学观察进行形态学鉴定；通过PCR扩增真菌核糖体基因转录间隔区序列并测序，并将序列在GenBank上进行同源性比对和构建系统系统发育树等方法进行分子鉴定，鉴定出这26株真菌分属链格孢属、镰孢属、黑孢属、刺盘孢属、匍柄霉属等5个属。

Employ single-member expert docking, a storekeeper for chemical products and medicines such as knowledge of special product identification, characteristics such as the training of basic knowledge to improve the staff's identification of goods and special recognition.

聘请专家对接单员、仓库保管员进行化工产品知识和药品等特殊产品标识、特性等基本知识的培训，提高员工对特殊货物的辨别和识别能力。

The identification results of four typical flow regimes of air-water two-phase flow in horizontal pipe show that the approach of neural network identification based on EMD extracting energy parameter is superior to that based on wavelet packet, and can identify flow regime accurately and effectively.

对水平管内空气-水两相流4种典型流型的识别结果表明，EMD能量比小波包能量特征具有更高的流型识别率，可以准确、有效地识别流型。

A Predictive Control algorithm is presented by incorporation of online identification and offline identification. Then aiming for the heat-exchanger outlet temperature plant of city hot-water networks and melting salt temperature plant of fixed bed reactor, the computer simulation study is made.

提出了在线辨识和离线辨识相结合的预测控制算法，并且针对城市热水网加热器出口水温控制对象和固定床反应器温度控制对象进行了仿真研究。

Objective:To compare the response control and attention of Schizophrenic patients with that of the healthy controls by Integrated Visual and Auditorycontinuous performance test. To quantitate the impairment of cognitive function in patients, and to explore the relationship between cognitive function and the severity of the disorder.

目的：探讨精神分裂症病人在IVA持续操作测试中的反应控制能力、注意力等，并与健康人进行比较，从而量化精神分裂症病人的认知功能损害，并进一步探讨其认知功能与疾病严重程度的关系。

Main effective factors including subcooling degree, mechanical vibration, gas hydrate reformation, environment temperature, noncondensing gas and surfactant are analyzed.

指出过冷度、机械振动、重复生成水合物、环境温度、不凝性气体、添加剂是影响气体水合物生成的主要因素，还对R152a水合物的放冷进行了实验研究。