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Results The full-length variant-specific surface antigen gone fragment from G. lamblia was found to be 2 142 bp, encoding a 713 amino acid polypeptide and contained a single open reading frame.

结果 中国人源蓝氏贾第鞭毛虫基因全长为2 142 bp，编码1条长为713个氨基酸残基的多肽链，含有一个简单的开放阅读框。

By the methods of molecular biology, enzymology, chemistry, physics the project was carried out. The genes encoding leucyl- and arginyl-tRNA synthetase , and tRNA and tRNA from Escherichia coli were cloned and overexpressed in E.

以大肠杆菌亮氨酰tRNA合成酶和精氨酰tRNA合成酶为对象用分子生物学、酶学、酶动力学、化学、物理学的方法进行该项研究。

Objective To solve the difficulties of identification of Sarcosaphagous flies such as Lucilia sericata and Lucilia cuprina which could not be identified by analyzing the 278 bp and 635 bp regions of the gene encoding for cytochrome oxidase subunitⅠand Ⅱ in mtDNA.

目的通过mtDNA上16SrDNA中551bp基因序列分析，解决依据COI和COII上278bp和635bp基因序列难以鉴别丝光绿蝇、铜绿蝇种类的难题，为法医学嗜尸性苍蝇种类的鉴别提供依据。

In the present report, two hypersensitive response initiation systems were employed to study the time_course expression induced by Avr / Cf interaction of the genes encoding Pti4, Pti5 and Pti6 which interact directly with Pto:(1) Seeds of tomato Lycopersicon esculentum Mill.

采用两种过敏性反应(hypersensitiveresponse ，HR)产生系统研究了Pto互作蛋白Pti4、Pti5和Pti6编码基因在Avr/Cf互作中的时序表达：(1)通过杂交方法获取同含互补基因对Avr4 /Cf_4和Avr9/Cf_9的番茄 Lycoper siconesculentumMill。

This strain is lysogenic for lambda-DE3(4), which contains the T7 bacteriophage gene1, encoding T7 RNA polymerase under the control of the lac UV5 promoter.

此类菌株为lambda-DE3(4)，溶源性，包含有T7细菌噬菌体基因1，它编码受制于UV5启动子的T7RNA聚合酶。

To localize the functional positions of biomolecules in ciliates cell, we constructed a macronuclear artificial chromosome containing a gene encoding red fluorescence protein based on the structural characteristics of ciliates chromosome.

为了能够有效地分析生物大分子在该细胞中的功能作用位点，本研究根据该生物染色体结构的特征，构建了含有红色荧光蛋白的大核人工染色体EoMAC_R，并与之前构建的含绿色荧光的大核染色体EoMAC_G一起，对蛋白质合成终止有关的3个重要因子核糖体大亚基蛋白L11、多肽链释放因子eRF1和eRF3在八肋游仆虫细胞中进行了荧光共定位分析。

RbcL gene was obtained by polymerase chain reaction and cloned into pDM19-T vector. The positive clone identified by PCR was sequenced. The nucleotide sequence was 1266 bp long, including an ORF of 1031bp, encoding a putative 343 amino acids. The deduced amino acid sequence had a high identity above 94.13% with that of tobacco, spinach, sweet potato, corn, Arabidopsis thaliana, Oryza sativa, Vitis vinifera, Marchantia polymorpha and Citrus paradise.

阳性克隆鉴定后进行测序，序列分析结果表明：该基因片断长为1266 bp，包括1031 bp的编码区序列，编码343个氨基酸；其编码区氨基酸与烟草、菠菜、玉米、甘薯、拟南芥、水稻、葡萄、地钱和葡萄柚等9个物种的同源性94.13%以上，并构建了它们间的亲缘关系树。

Similar to other flaviviruses, JEV contains a single-stranded, positive-sense RNA of approxi- mately 11 kilobases in length, which has a single open reading frame encoding a polyprotein.

WHO正在呼吁改善和研制新型乙脑疫苗，乙脑病毒的E蛋白与病毒和细胞受体结合，膜融合有关，也是引起中和抗体及宿主保护的主要免疫蛋白。

METHODS:A PCR protocol was designed to detect the Schistosoma japonicum 5D gene encoding an immunogenic miracidial antigen in cercariae、eggs and adult worms.

通过引物1、引物2单扩增，即能检测到单个虫卵、单个尾蚴或针尖大小的成虫组织。采用Nest－PCR和非同位素探针能将敏感性进一步提高。

Here we show, for eight heterosexual transmission pairs, that recipient viruses were monophyletic, encoding compact, glycan-restricted envelope glycoproteins.

作者发现在8对异性传播例子中被感染者病毒是单源的，编码紧凑的聚糖－限制型外膜糖蛋白。

Pachomius manual labour was organized as an essential part of the monastic life; and since it is a principle of the monks as distinguished from the mendicants, that the body shall be self-supporting, external work of one sort or another has been an inevitable part of the life ever since.

根据圣pachomius体力劳动组织作为一个重要组成部分寺院生活；以来，这是一个原则的和尚，作为有别于乞丐，该机构应自支撑，对外工作的这种或那种已一个必然的一部分，生活至今。

In order to study the effects of parametrical rolling on the ship movement and tilts, in allusion to ship's navigating in regular following waves, the equation of ship rolling motion is transformed into normal Mathieu equation by proper variable replacement when strong parametrical excitation in restoring moment varied according to sine or cosine wave.

为研究参数横摇对船舶运动和倾覆的影响，通过对船在规则波上随浪航行时，恢复力矩中出现的1个以正余弦规律变化的强参数激励项进行适当的变量置换，将船舶的横摇运动方程转换成标准形式的Mathieu方程。