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This article reviews the functions of VLCFAs and cuticular wax, and the recent progress in cloning and characterization of genes encoding enzymes involved in catalyzing VLCFAs and cuticular wax biosynthesis. The problems existing in researches of wax genes are also discussed.

文章就VLCFAs及角质层蜡质合成代谢途径中相关酶基因研究进展方面做了综述，并对植物蜡质基因研究中存在的问题提出一些看法。

Sequencing of this 4.3kb DNA fragment revealed that there is a β-glucosidase gene urnbgl3A with an ORF of 1956bp, encoding product shared 63% identity and 79% similarity with a beta-glucosidase (GenBank NO:AAX16378.1) of uncultured murine large bowel bacterium BAC 31B, and shared 50% identity and 67% similarity with a beta-glucosidase-related glycosidases (GenBank NO:ZP-00308419.1) of Cytophaga hutchinsonii.

测序结果表明在4.3kb的片段上有一个全长为1956bp的ORF，编码一个可能的β-葡萄糖苷酶基因umbgl3A，其编码产物与一个来源於小鼠大肠未培养细菌的β-葡萄糖苷酶(GenBank Acession NO：AAX16378.1)一致性为63%、相似性为79%；与哈氏噬纤维菌的β-葡萄糖苷酶(GenBank Acession NO：ZP-00308419)的一致性为50%、相似性为67%。

Total mRNA was extracted from a venom gland of snake Daboia russellii siamensis following the manufacturers protocol of the PolyATtract System 1000 kit purchased from Promega Biotech. cDNAs encoding C-type lectins were amplified by RT-PCR and subcloned into a pMD18-T vector.

按照Promega公司的mRNA提取试剂盒操作手册，从圆斑蝰蛇的毒腺中提取mRNA；利用RT-PCR的方法进行体外扩增，获得C-型凝集素蛋白的基因，克隆到pMD18-T载体中。

The structure of the gene encoding the DAP was also analyzed by two PCR.

运用PCR技术，我们又研究了这个DAP的基因结构。

Results The whole genome of CVB4 jlu06 strain consisted of 7 395 nucleotides encoding 2183 amino acids, which showed high homologies to other CVB4 strains, with the mutations of 10 amino acids. Phylogenetic analysis proved that CVB4 jlu06 strain showed close relationship to HumanCB4 and POLG_CXB4 strains while showed remote relationship to CXB2 strain. Unlike other non-diabetogenic strains, CVB4 jlu06 strain showed common amino acids with diabetogenic strains at 4 sites in 3A region of non-structural protein.

结果 CVB4 jlu06株基因组全长7395个核苷酸，编码2183个氨基酸，与其他CVB4株的同源性较高，且发生了10个氨基酸的变异，与HumanCB4和POLG_CXB4亲缘关系较近，与CXB2亲缘关系较远；CVB4 jlu06株与具有潜在致糖尿病性病毒株，在非结构蛋白3A区4个位点有共同的氨基酸，与非致病毒株不同。

We found that the right dorsolateral prefrontal cortex was crucial for the retrieval of the encoded pictorial information, whereas the left DLPFC was involved in encoding operations.

我们发现右背外侧的额前皮质对编码的图片信息检索至关重要，而右背外侧的额前皮质与编码操作相关。

The phase-key in the doudle random-phase encoding system is recorded as digital holograms for this purpose.

提出把双随机相位编码中的相位密钥记录为数字全息图，以实现密钥的复制。

In this research work, the technology of microbial conversion was studied in order to establish an environment friendly method for the production of doxorubicin. A doxA gene encoding Daunorubicin C-14 hydroxylase was cloned from a daunorubicin-producing strain Streptomyces coeruleorubidus SIPI 1482. Some plasmids for the expression of doxA gene were constructed and transformed into S.

本研究对微生物转化法替代现有工艺生产阿霉素进行了探索，从柔红霉素产生菌天蓝淡红链霉菌SIPI 1482中克隆了柔红霉素C-14羟化酶基因，构建了doxA基因的链霉菌表达质粒，导入变铅青链霉菌TK24中获得了柔红霉素转化基因工程菌，并研究了doxA基因在工程菌中的表达，最后还对工程菌转化柔红霉素生成阿霉素的发酵工艺进行了初步研究。

Mutations in DKC1 gene encoding dyskerin are responsible for the X-linked dyskeratosis congenita.

DKC存在X染色体隐性、常染色体显性和隐性三种遗传方式。

Germ-line mutations in the genes hTERT and hTR, encoding telomerase reverse transcriptase and telomerase RNA, respectively, cause autosomal dominant dyskeratosis congenita, a rare hereditary disorder associated with premature death from aplastic anemia and pulmonary fibrosis.

分别编码端粒末端转移酶反转录酶和端粒酶RNA的hTERT和hTR基因的种系突变导致常染色体显性先天性角化不良，它是一种罕见的因为再生障碍性贫血和肺纤维化而早亡的遗传性疾病。

Pachomius manual labour was organized as an essential part of the monastic life; and since it is a principle of the monks as distinguished from the mendicants, that the body shall be self-supporting, external work of one sort or another has been an inevitable part of the life ever since.

根据圣pachomius体力劳动组织作为一个重要组成部分寺院生活；以来，这是一个原则的和尚，作为有别于乞丐，该机构应自支撑，对外工作的这种或那种已一个必然的一部分，生活至今。

In order to study the effects of parametrical rolling on the ship movement and tilts, in allusion to ship's navigating in regular following waves, the equation of ship rolling motion is transformed into normal Mathieu equation by proper variable replacement when strong parametrical excitation in restoring moment varied according to sine or cosine wave.

为研究参数横摇对船舶运动和倾覆的影响，通过对船在规则波上随浪航行时，恢复力矩中出现的1个以正余弦规律变化的强参数激励项进行适当的变量置换，将船舶的横摇运动方程转换成标准形式的Mathieu方程。